Method for construction of normalized cDNA libraries

Soares, M B; Efstratiadis, A

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Title: Method for construction of normalized cDNA libraries

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Abstract

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form. The method comprises: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.

Inventors:: Soares, M B; Efstratiadis, A

Issue Date:: Tue Jan 09 00:00:00 EST 1996

Research Org.:: Columbia Univ., New York, NY (United States)

OSTI Identifier:: 170463

Patent Number(s):: 5482845

Application Number:: PAN: 8-126,594

Assignee:: Columbia Univ., New York, NY (United States)

DOE Contract Number:: FG02-91ER61233

Resource Type:: Patent

Resource Relation:: Other Information: PBD: 9 Jan 1996

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA; GENETIC MAPPING; MOLECULAR STRUCTURE; BIOCHEMISTRY; MOLECULAR BIOLOGY

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                    Soares, M B, and Efstratiadis, A. Method for construction of normalized cDNA libraries.  United States: N. p., 1996. 
        Web.

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                    Soares, M B, & Efstratiadis, A. Method for construction of normalized cDNA libraries.  United States.

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                    Soares, M B, and Efstratiadis, A. Tue .  
        "Method for construction of normalized cDNA libraries".  United States.

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@article{osti_170463,

  title        = {Method for construction of normalized cDNA libraries},

  author       = {Soares, M B and Efstratiadis, A},

  abstractNote = {This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form. The method comprises: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Jan 09 00:00:00 EST 1996},

  month        = {Tue Jan 09 00:00:00 EST 1996}

}

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Full Text Available
Method for construction of normalized cDNA libraries

Patent Soares, Marcelo B [New York, NY]; Efstratiadis, Argiris [Englewood, NJ]

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.
Full Text Available
Method for construction of normalized cDNA libraries

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This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.
Full Text Available

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