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Title: Method for construction of normalized cDNA libraries

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form. The method comprises: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.
Inventors:
;
Issue Date:
OSTI Identifier:
170463
Assignee:
Columbia Univ., New York, NY (United States) PTO; SCA: 550400; 550200; PA: EDB-96:030490; SN: 96001517580
Patent Number(s):
US 5,482,845/A/
Application Number:
PAN: 8-126,594
Contract Number:
FG02-91ER61233
Resource Relation:
Other Information: PBD: 9 Jan 1996
Research Org:
Columbia University
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA; GENETIC MAPPING; MOLECULAR STRUCTURE; BIOCHEMISTRY; MOLECULAR BIOLOGY

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