Multiple sort flow cytometer

Engh, G van den; Esposito, R J

Title: Multiple sort flow cytometer

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Abstract

A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream. Once identified, verified and timed in the sheath flow, each event is independently tagged upon separation from the flow by an electrical charge of +60, +120, or +180 volts and passed through oppositely charged deflection plates with ground planes to yield a focused six way deflection of at least six events in a narrow plane. 8 figs.

Inventors:: Engh, G van den; Esposito, R J

Issue Date:: 1996-01-09

Research Org.:: Univ. of California (United States)

OSTI Identifier:: 170461

Patent Number(s):: 5483469

Application Number:: PAN: 8-100,762

Assignee:: Univ. of California, Oakland, CA (United States)

DOE Contract Number:: W-7405-ENG-48

Resource Type:: Patent

Resource Relation:: Other Information: PBD: 9 Jan 1996

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; CELL FLOW SYSTEMS; DESIGN; ANIMAL CELLS; CYTOLOGICAL TECHNIQUES; PLANT CELLS

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                    Engh, G van den, and Esposito, R J. Multiple sort flow cytometer.  United States: N. p., 1996. 
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                    Engh, G van den, & Esposito, R J. Multiple sort flow cytometer.  United States.

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                    Engh, G van den, and Esposito, R J. Tue .  
        "Multiple sort flow cytometer".  United States.

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@article{osti_170461,

  title        = {Multiple sort flow cytometer},

  author       = {Engh, G van den and Esposito, R J},

  abstractNote = {A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream. Once identified, verified and timed in the sheath flow, each event is independently tagged upon separation from the flow by an electrical charge of +60, +120, or +180 volts and passed through oppositely charged deflection plates with ground planes to yield a focused six way deflection of at least six events in a narrow plane. 8 figs.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1996},

  month        = {1}

}

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A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream. Once identified, verified and timed in the sheath flow, each event is independently tagged upon separation from the flow by an electrical charge of +60, +120, or +180 volts and passed through oppositely charged deflection plates with ground planes to yield a focused six way deflection of at least six events in a narrow plane.
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