skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: RNA-guided transcriptional regulation

Abstract

Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

Inventors:
; ;
Issue Date:
Research Org.:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1650849
Patent Number(s):
10640789
Application Number:
15/049,451
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Resource Relation:
Patent File Date: 02/22/2016
Country of Publication:
United States
Language:
English

Citation Formats

Church, George M., Mali, Prashant G., and Esvelt, Kevin Michael. RNA-guided transcriptional regulation. United States: N. p., 2020. Web.
Church, George M., Mali, Prashant G., & Esvelt, Kevin Michael. RNA-guided transcriptional regulation. United States.
Church, George M., Mali, Prashant G., and Esvelt, Kevin Michael. Tue . "RNA-guided transcriptional regulation". United States. https://www.osti.gov/servlets/purl/1650849.
@article{osti_1650849,
title = {RNA-guided transcriptional regulation},
author = {Church, George M. and Mali, Prashant G. and Esvelt, Kevin Michael},
abstractNote = {Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {5}
}

Patent:

Save / Share:

Works referenced in this record: