Increased ethanol production by thermophilic microorganisms with deletion of individual hfs hydrogenase subunits
Abstract
Disclosed are methods for engineering bacteria, for example, Thermoanaerobacterium saccharolyticum, that convert biomass to ethanol at high yield by deleting a single gene. Deletion of subunit A or subunit B of the hfs hydrogenase, but not deletion of subunit C or subunit D, results in an increase in ethanol yield.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1650762
- Patent Number(s):
- 10619172
- Application Number:
- 15/656,665
- Assignee:
- Trustees of Dartmouth College (Hanover, NH)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC05-00OR22725
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 07/21/2017
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS
Citation Formats
Eminoglu, Aysenur, Olson, Daniel G., and Lynd, Lee R. Increased ethanol production by thermophilic microorganisms with deletion of individual hfs hydrogenase subunits. United States: N. p., 2020.
Web.
Eminoglu, Aysenur, Olson, Daniel G., & Lynd, Lee R. Increased ethanol production by thermophilic microorganisms with deletion of individual hfs hydrogenase subunits. United States.
Eminoglu, Aysenur, Olson, Daniel G., and Lynd, Lee R. Tue .
"Increased ethanol production by thermophilic microorganisms with deletion of individual hfs hydrogenase subunits". United States. https://www.osti.gov/servlets/purl/1650762.
@article{osti_1650762,
title = {Increased ethanol production by thermophilic microorganisms with deletion of individual hfs hydrogenase subunits},
author = {Eminoglu, Aysenur and Olson, Daniel G. and Lynd, Lee R.},
abstractNote = {Disclosed are methods for engineering bacteria, for example, Thermoanaerobacterium saccharolyticum, that convert biomass to ethanol at high yield by deleting a single gene. Deletion of subunit A or subunit B of the hfs hydrogenase, but not deletion of subunit C or subunit D, results in an increase in ethanol yield.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {4}
}
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