Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid
Abstract
Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.
- Inventors:
- Issue Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1637827
- Patent Number(s):
- 10590477
- Application Number:
- 15/717,524
- Assignee:
- National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- NA0003525
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 09/27/2017
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Koh, Chung-Yan, Light, Yooli Kim, Piccini, Matthew Ernest, and Singh, Anup K. Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid. United States: N. p., 2020.
Web.
Koh, Chung-Yan, Light, Yooli Kim, Piccini, Matthew Ernest, & Singh, Anup K. Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid. United States.
Koh, Chung-Yan, Light, Yooli Kim, Piccini, Matthew Ernest, and Singh, Anup K. Tue .
"Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid". United States. https://www.osti.gov/servlets/purl/1637827.
@article{osti_1637827,
title = {Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid},
author = {Koh, Chung-Yan and Light, Yooli Kim and Piccini, Matthew Ernest and Singh, Anup K.},
abstractNote = {Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {3}
}
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