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Title: Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid

Abstract

Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.

Inventors:
; ; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1637827
Patent Number(s):
10590477
Application Number:
15/717,524
Assignee:
National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
NA0003525
Resource Type:
Patent
Resource Relation:
Patent File Date: 09/27/2017
Country of Publication:
United States
Language:
English

Citation Formats

Koh, Chung-Yan, Light, Yooli Kim, Piccini, Matthew Ernest, and Singh, Anup K. Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid. United States: N. p., 2020. Web.
Koh, Chung-Yan, Light, Yooli Kim, Piccini, Matthew Ernest, & Singh, Anup K. Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid. United States.
Koh, Chung-Yan, Light, Yooli Kim, Piccini, Matthew Ernest, and Singh, Anup K. Tue . "Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid". United States. https://www.osti.gov/servlets/purl/1637827.
@article{osti_1637827,
title = {Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid},
author = {Koh, Chung-Yan and Light, Yooli Kim and Piccini, Matthew Ernest and Singh, Anup K.},
abstractNote = {Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {3}
}

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