skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid

Abstract

Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.

Inventors:
; ; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1637827
Patent Number(s):
10590477
Application Number:
15/717,524
Assignee:
National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
NA0003525
Resource Type:
Patent
Resource Relation:
Patent File Date: 09/27/2017
Country of Publication:
United States
Language:
English

Citation Formats

Koh, Chung-Yan, Light, Yooli Kim, Piccini, Matthew Ernest, and Singh, Anup K. Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid. United States: N. p., 2020. Web.
Koh, Chung-Yan, Light, Yooli Kim, Piccini, Matthew Ernest, & Singh, Anup K. Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid. United States.
Koh, Chung-Yan, Light, Yooli Kim, Piccini, Matthew Ernest, and Singh, Anup K. Tue . "Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid". United States. https://www.osti.gov/servlets/purl/1637827.
@article{osti_1637827,
title = {Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid},
author = {Koh, Chung-Yan and Light, Yooli Kim and Piccini, Matthew Ernest and Singh, Anup K.},
abstractNote = {Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {3}
}

Patent:

Save / Share:

Works referenced in this record:

Diagnostic tests and kits for Clostridium difficile
patent, January 2003


Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto
patent, January 2007


Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
patent, February 2015


Centripetally-motivated microfluidics system for performing in vitro hybridization and amplification of nucleic acids
patent, February 2008


Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system
patent, November 2001


Separation of solid and liquid components of mixtures
patent, November 1978


Devices, systems, and methods for conducting sandwich assays using sedimentation
patent, February 2015


Microfluidic devices, systems, and methods for quantifying particles using centrifugal force
patent, November 2015


Apparatus for precise transfer and manipulation of fluids by centrifugal and or capillary forces
patent, December 2012


Protecting agent
patent-application, August 2005


Systems, devices, and methods for agglutination assays using sedimentation
patent, January 2016


Assay of blood or other biologic samples for target analytes
patent, June 1997


Toxin activity assays, devices, methods and systems therefor
patent, April 2016


Centrifugal hematology disposable
patent, November 2000


Microfluidic particle-analysis systems
patent, December 2007


Heterogeneous binding assay
patent, April 1987


Monolithic microfluidic concentrators and mixers
patent, May 2005


Assay system and method for conducting assays
patent, March 1999


Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
patent, April 2016