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Title: Enzyme scaffolds and methods of use

Abstract

Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1632628
Patent Number(s):
10577633
Application Number:
15/467,340
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Patent
Resource Relation:
Patent File Date: 03/23/2017
Country of Publication:
United States
Language:
English

Citation Formats

Bomble, Yannick J., Himmel, Michael E., Linger, Jeffrey, Brunecky, Roman, and Aikens, John. Enzyme scaffolds and methods of use. United States: N. p., 2020. Web.
Bomble, Yannick J., Himmel, Michael E., Linger, Jeffrey, Brunecky, Roman, & Aikens, John. Enzyme scaffolds and methods of use. United States.
Bomble, Yannick J., Himmel, Michael E., Linger, Jeffrey, Brunecky, Roman, and Aikens, John. Tue . "Enzyme scaffolds and methods of use". United States. https://www.osti.gov/servlets/purl/1632628.
@article{osti_1632628,
title = {Enzyme scaffolds and methods of use},
author = {Bomble, Yannick J. and Himmel, Michael E. and Linger, Jeffrey and Brunecky, Roman and Aikens, John},
abstractNote = {Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {3}
}

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