Enzyme scaffolds and methods of use
Abstract
Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays.
- Inventors:
- Issue Date:
- Research Org.:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1632628
- Patent Number(s):
- 10577633
- Application Number:
- 15/467,340
- Assignee:
- Alliance for Sustainable Energy, LLC (Golden, CO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC36-08GO28308
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 03/23/2017
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Bomble, Yannick J., Himmel, Michael E., Linger, Jeffrey, Brunecky, Roman, and Aikens, John. Enzyme scaffolds and methods of use. United States: N. p., 2020.
Web.
Bomble, Yannick J., Himmel, Michael E., Linger, Jeffrey, Brunecky, Roman, & Aikens, John. Enzyme scaffolds and methods of use. United States.
Bomble, Yannick J., Himmel, Michael E., Linger, Jeffrey, Brunecky, Roman, and Aikens, John. Tue .
"Enzyme scaffolds and methods of use". United States. https://www.osti.gov/servlets/purl/1632628.
@article{osti_1632628,
title = {Enzyme scaffolds and methods of use},
author = {Bomble, Yannick J. and Himmel, Michael E. and Linger, Jeffrey and Brunecky, Roman and Aikens, John},
abstractNote = {Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {3}
}
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