Enzyme scaffolds and methods of use

Title: Enzyme scaffolds and methods of use

Abstract

Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays.

Inventors:: Bomble, Yannick J.; Himmel, Michael E.; Linger, Jeffrey; Brunecky, Roman; Aikens, John

Issue Date:: 2020-03-03

Research Org.:: National Renewable Energy Lab. (NREL), Golden, CO (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1632628

Patent Number(s):: 10577633

Application Number:: 15/467,340

Assignee:: Alliance for Sustainable Energy, LLC (Golden, CO)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y101/01006 - Glycerol dehydrogenase
C12Y106/03001 - NAD
C12Y101/01086 - Ketol-acid reductoisomerase

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P7/42 - Hydroxy-carboxylic acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y106/03 - with oxygen as acceptor

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N11/18 - Multi-enzyme systems
C12N9/1022 - {transferring aldehyde or ketonic groups
C12N9/0006 - {acting on CH-OH groups as donors

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y202/01006 - Acetolactate synthase

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/0036 - {acting on NADH or NADPH
C12N15/62 - DNA sequences coding for fusion proteins

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K14/195 - from bacteria

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P7/18 - polyhydric

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/0008 - {acting on the aldehyde or oxo group of donors

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y102/01003 - Aldehyde dehydrogenase
C12Y101/01076 -
C12Y102/01 - with NAD+ or NADP+ as acceptor

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N11/06 - attached to the carrier via a bridging agent
C12N11/00 - Carrier-bound or immobilised enzymes

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DOE Contract Number:: AC36-08GO28308

Resource Type:: Patent

Resource Relation:: Patent File Date: 03/23/2017

Country of Publication:: United States

Language:: English

Citation Formats


                    Bomble, Yannick J., Himmel, Michael E., Linger, Jeffrey, Brunecky, Roman, and Aikens, John. Enzyme scaffolds and methods of use.  United States: N. p., 2020. 
        Web.

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                    Bomble, Yannick J., Himmel, Michael E., Linger, Jeffrey, Brunecky, Roman, & Aikens, John. Enzyme scaffolds and methods of use.  United States.

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                    Bomble, Yannick J., Himmel, Michael E., Linger, Jeffrey, Brunecky, Roman, and Aikens, John. Tue .  
        "Enzyme scaffolds and methods of use".  United States.  https://www.osti.gov/servlets/purl/1632628.

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@article{osti_1632628,

  title        = {Enzyme scaffolds and methods of use},

  author       = {Bomble, Yannick J. and Himmel, Michael E. and Linger, Jeffrey and Brunecky, Roman and Aikens, John},

  abstractNote = {Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2020},

  month        = {3}

}

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Works referenced in this record:

Method for analyzing and synthesizing functional polypeptide sequences for assembly into enhanced or multi-functional proteins
patent, August 1990

Jasin, Maria; Schimmel, Paul R.
US Patent Document 4,952,501
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4952501

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Title: Enzyme scaffolds and methods of use

Abstract

Citation Formats

Method for analyzing and synthesizing functional polypeptide sequences for assembly into enhanced or multi-functional proteins patent, August 1990

Method for analyzing and synthesizing functional polypeptide sequences for assembly into enhanced or multi-functional proteins
patent, August 1990