Title: Methods, systems, and compositions for studying solvent accessibility and three-dimensional structure of biological molecules

Abstract

This disclosure provides methods, systems, and compositions of matter for studying solvent accessibility and three-dimensional structure of biological molecules. A plasma can be used to generate marker radicals, which can interact with a biological molecule and mark the solvent-accessible portions of the biological molecule.

Inventors:

Sussman, Michael R.; Shohet, J. Leon; Choudhury, Faraz A.; Blatz, Joshua M.; Minkoff, Benjamin B.; Benjamin, Daniel I.

Issue Date:

Tue Feb 25 00:00:00 EST 2020

Research Org.:

Wisconsin Alumni Research Foundation, Madison, WI (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1632612

Patent Number(s):

10571460

Application Number:

15/600,352

Assignee:

Wisconsin Alumni Research Foundation (Madison, WI)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J19/088 - {giving rise to electric discharges
B01J2219/0809 - employing two or more electrodes
B01J2219/0894 - Processes carried out in the presence of a plasma

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N1/28 - Preparing specimens for investigation {including physical details of
G01N2333/765 - Serum albumin, e.g. HSA
G01N2333/90216 - acting on a heme group of donors
G01N2333/91215 - with a definite EC number
G01N2440/00 - Post-translational modifications [PTMs] in chemical analysis of biological material
G01N33/487 - of liquid biological material
G01N33/50 - Chemical analysis of biological material, e.g. blood, urine
G01N33/6848 - {Methods of protein analysis involving mass spectrometry}

H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS

H01J49/0031 - {Step by step routines describing the use of the apparatus

H - ELECTRICITY H05 - ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR H05H - PLASMA TECHNIQUE

H05H1/24 - Generating plasma {

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DOE Contract Number:  

FG02-88ER13938

Resource Type:

Patent

Resource Relation:

Patent File Date: 05/19/2017

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY