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Title: Methods of introducing nucleic acids into cellular DNA

Abstract

A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.

Inventors:
; ; ;
Issue Date:
Research Org.:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1632604
Patent Number(s):
10570400
Application Number:
15/426,527
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Resource Relation:
Patent File Date: 02/07/2017
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lajoie, Marc J., Gregg, Christopher J., Mosberg, Joshua A., and Church, George M. Methods of introducing nucleic acids into cellular DNA. United States: N. p., 2020. Web.
Lajoie, Marc J., Gregg, Christopher J., Mosberg, Joshua A., & Church, George M. Methods of introducing nucleic acids into cellular DNA. United States.
Lajoie, Marc J., Gregg, Christopher J., Mosberg, Joshua A., and Church, George M. Tue . "Methods of introducing nucleic acids into cellular DNA". United States. https://www.osti.gov/servlets/purl/1632604.
@article{osti_1632604,
title = {Methods of introducing nucleic acids into cellular DNA},
author = {Lajoie, Marc J. and Gregg, Christopher J. and Mosberg, Joshua A. and Church, George M.},
abstractNote = {A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {2}
}

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Works referenced in this record:

Multiplex automated genome engineering
patent, April 2012