Methods of introducing nucleic acids into cellular DNA
Abstract
A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.
- Inventors:
- Issue Date:
- Research Org.:
- Harvard Univ., Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1632604
- Patent Number(s):
- 10570400
- Application Number:
- 15/426,527
- Assignee:
- President and Fellows of Harvard College (Cambridge, MA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG02-02ER63445
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 02/07/2017
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Lajoie, Marc J., Gregg, Christopher J., Mosberg, Joshua A., and Church, George M. Methods of introducing nucleic acids into cellular DNA. United States: N. p., 2020.
Web.
Lajoie, Marc J., Gregg, Christopher J., Mosberg, Joshua A., & Church, George M. Methods of introducing nucleic acids into cellular DNA. United States.
Lajoie, Marc J., Gregg, Christopher J., Mosberg, Joshua A., and Church, George M. Tue .
"Methods of introducing nucleic acids into cellular DNA". United States. https://www.osti.gov/servlets/purl/1632604.
@article{osti_1632604,
title = {Methods of introducing nucleic acids into cellular DNA},
author = {Lajoie, Marc J. and Gregg, Christopher J. and Mosberg, Joshua A. and Church, George M.},
abstractNote = {A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {2}
}
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Works referenced in this record:
Multiplex automated genome engineering
patent, April 2012
- Church, George M.; Wang, Harris H.; Isaacs, Farren J.
- US Patent Document 8,153,432