DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Hybrid polyketide synthases

Abstract

The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.

Inventors:
; ; ; ; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1600351
Patent Number(s):
10494625
Application Number:
15/144,727
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 05/02/2016
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Fortman, Jeffrey L., Hagen, Andrew, Katz, Leonard, Keasling, Jay D., Poust, Sean, Zhang, Jingwei, and Zotchev, Sergey. Hybrid polyketide synthases. United States: N. p., 2019. Web.
Fortman, Jeffrey L., Hagen, Andrew, Katz, Leonard, Keasling, Jay D., Poust, Sean, Zhang, Jingwei, & Zotchev, Sergey. Hybrid polyketide synthases. United States.
Fortman, Jeffrey L., Hagen, Andrew, Katz, Leonard, Keasling, Jay D., Poust, Sean, Zhang, Jingwei, and Zotchev, Sergey. Tue . "Hybrid polyketide synthases". United States. https://www.osti.gov/servlets/purl/1600351.
@article{osti_1600351,
title = {Hybrid polyketide synthases},
author = {Fortman, Jeffrey L. and Hagen, Andrew and Katz, Leonard and Keasling, Jay D. and Poust, Sean and Zhang, Jingwei and Zotchev, Sergey},
abstractNote = {The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {12}
}

Works referenced in this record:

Tolerance and Specificity of Polyketide Synthases
journal, June 1999


Biosynthesis of Nonribosomal Peptides
journal, October 2004


Novel avermectins produced by mutational biosynthesis.
journal, January 1991


Predictive, structure-based model of amino acid recognition by nonribosomal peptide synthetase adenylation domains
journal, March 2000


Expanding the biosynthetic repertoire of plant type III polyketide synthases by altering starter molecule specificity
journal, April 2002


Polyketide biosynthesis: a millennium review
journal, January 2001


Polyketide synthesis in vitro on a modular polyketide synthase
journal, September 1995


Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338
journal, March 2007


Engineering Broader Specificity into an Antibiotic-Producing Polyketide Synthase
journal, January 1998


Producing dicarboxylic acids using polyketide synthases
patent, October 2013


Stereochemical Determination and Complex Biosynthetic Assembly of Etnangien, a Highly Potent RNA Polymerase Inhibitor from the Myxobacterium Sorangium cellulosum
journal, October 2008


Unusual Chemistry in the Biosynthesis of the Antibiotic Chondrochlorens
journal, January 2009


Host Cells and Methods for Producing Diacid Compounds
patent-application, October 2013


Spirofungin, a New Antifungal Antibiotic from Streptomyces violaceusniger Tue 4113.
journal, January 1998


Automated genome mining for natural products
journal, January 2009


NRPS-PKS: a knowledge-based resource for analysis of NRPS/PKS megasynthases
journal, July 2004


Crystal structure of DhbE, an archetype for aryl acid activating domains of modular nonribosomal peptide synthetases
journal, September 2002


Alteration of the Substrate Specificity of a Modular Polyketide Synthase Acyltransferase Domain through Site-Specific Mutations
journal, December 2001