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Title: Microorganisms and methods for the production of fatty acids and fatty acid derived products


This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.

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Issue Date:
Research Org.:
Cargill, Inc., Wayzata, MN (United States)
Sponsoring Org.:
OSTI Identifier:
Patent Number(s):
Application Number:
Cargill, Incorporated (Wayzata, MN)
Patent Classifications (CPCs):
DOE Contract Number:  
Resource Type:
Resource Relation:
Patent File Date: 03/29/2016
Country of Publication:
United States

Citation Formats

Liao, Hans, Spindler, Eileen, Warner, Joseph R., Louie, Michael, Ribble, Wendy, Prather, Brittany, Evans, Ron, Lipscomb, Tanya E. W., and Lynch, Michael D. Microorganisms and methods for the production of fatty acids and fatty acid derived products. United States: N. p., 2019. Web.
Liao, Hans, Spindler, Eileen, Warner, Joseph R., Louie, Michael, Ribble, Wendy, Prather, Brittany, Evans, Ron, Lipscomb, Tanya E. W., & Lynch, Michael D. Microorganisms and methods for the production of fatty acids and fatty acid derived products. United States.
Liao, Hans, Spindler, Eileen, Warner, Joseph R., Louie, Michael, Ribble, Wendy, Prather, Brittany, Evans, Ron, Lipscomb, Tanya E. W., and Lynch, Michael D. Tue . "Microorganisms and methods for the production of fatty acids and fatty acid derived products". United States.
title = {Microorganisms and methods for the production of fatty acids and fatty acid derived products},
author = {Liao, Hans and Spindler, Eileen and Warner, Joseph R. and Louie, Michael and Ribble, Wendy and Prather, Brittany and Evans, Ron and Lipscomb, Tanya E. W. and Lynch, Michael D.},
abstractNote = {This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {11}

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patent, August 2006

Catalyzed esterification process
patent, June 1995

Method for producing an organic acid
patent, December 2011

Treatment of biomass to obtain fermentable sugars
patent-application, February 2007

Purification of 3-Hydroxypropionic Acid from Crude Cell Broth and Production of Acrylamide
patent-application, October 2014

Production of Modified Fatty Acids in Plants
patent-application, July 2010

The IdhA Gene Encoding the Fermentative Lactate Dehydrogenase of Escherichia Coli
journal, January 1997

Compositions Comprising and Methods for Producing Beta-Hydroxy Fatty Acid Esters
patent-application, August 2014

Regulation of fatty acid biosynthesis in Escherichia coli.
journal, January 1993

Nucleotide sequence of the Escherichia coli cad operon: a system for neutralization of low extracellular pH.
journal, April 1992

Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase
journal, January 1988

Expression of microbial proteins in plants for production of plants with improved properties
patent-application, December 2003

Production Of Isoprenoids
patent-application, November 2008

Methods and Compositions for Producing Chemical Products from C. Phytofermentans
patent-application, July 2011

Extracellular Polyhydroxyalkanoates Produced By Genetically Engineered Microorganisms
patent-application, July 2011

Method For Determining Gene Knockouts
patent-application, January 2004

Genetic Modification for Production of 3-Hydroxypropionic Acid
patent-application, December 2012

Carboxylic acid recovery and methods related thereto
patent-application, November 2011

Development Of Sensitive Fret Sensors And Methods Of Using The Same
patent-application, February 2010

Cloning, crystallization and preliminary characterization of a β-carbonic anhydrase from Escherichia coli
journal, September 2000

Higher Fatty Acid Triester and Amide Derivative Having Diethylenetriamine-Type Metal Chelate Structure
patent-application, October 2009

Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
journal, June 2003

Method for the Production of Resveratrol in a Recombinant Oleaginous Microorganism
patent-application, March 2009

Method of Enhanced Bioproduction
patent-application, February 2015

Methods of making amino acids using E. coli transformed with csc genes
patent, November 2005

The commercial production of chemicals using pathway engineering
journal, December 2000

3-hydroxypropionic acid and other organic compounds
patent, December 2009

Engineering of Thermotolerant Bacillus Coagulans for Production of D(-)-Lactic Acid
patent-application, May 2012

Selective production of L-aspartic acid and L-phenylalanine by coupling reactions of aspartase and aminotransferase in Escherichia coli
journal, July 2000

Electroporation of Alcaligenes eutrophus with (mega) plasmids and genomic DNA fragments
journal, October 1994

Energy Efficient Methods To Produce Products
patent-application, August 2008

A Molecular Caliper Mechanism for Determining Very Long-Chain Fatty Acid Length
journal, August 2007

The Biotin Carboxylase-Biotin Carboxyl Carrier Protein Complex of Escherichia coli Acetyl-CoA Carboxylase
journal, June 2003

Lactic acid production, separation and/or recovery process
patent, April 1996

Method For Identifying A Biosynthetic Pathway Gene Product
patent-application, June 2007

3-Hydroxypropionic Acid and Other Organic Compounds
patent-application, September 2012

Acetyl-coA carboxylases
patent, September 2016

Method of Producing 3-Hydroxypropionic Acid Using Malonic Semialdehyde Reducing Pathway
patent-application, September 2012

Enhanced production of fatty acid derivatives
patent, February 2012

Reactions of β-lactones with potassium alkoxides and their complexes with 18-crown-6 in aprotic solvents
journal, July 1993

De novo biosynthetic pathways: rational design of microbial chemical factories
journal, October 2008

FolM, A New Chromosomally Encoded Dihydrofolate Reductase in Escherichia coli
journal, November 2003

3-phenylpropionate catabolism and the Escherichia coli oxidative stress response
journal, April 2005

Polyunsaturated fatty acid production in heterologous organisms using PUFA polyketide synthase systems
patent-application, October 2007

Methods Of Making Intermediates From Polyhydroxyalkanoates
patent-application, August 2003

Biosynthetic pathways for 3-hydroxypropionic acid production
journal, February 2009

A T42M Substitution in Bacterial 5-Enolpyruvylshikimate-3-phosphate Synthase (EPSPS) Generates Enzymes with Increased Resistance to Glyphosate
journal, January 2003

Process for the Biological Production of 1,3-propanediol With High Yield
patent-application, August 2004

Water-absorbing agent and production process therefor
patent, October 2001

Purification Of 3-Hydroxypropionic Acid From Crude Cell Broth And Production Of Acrylamide
patent-application, December 2013

Metabolically engineered microorganism useful for the production of 1,2-propanediol
patent-application, October 2010

3-hydroxypropionic acid and other organic compounds
patent-application, August 2007

Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli
journal, January 2008

Overproduction of a Functional Fatty Acid Biosynthetic Enzyme Blocks Fatty Acid Synthesis inEscherichia coli
journal, September 1998

Naturally Occurring Adenines within mRNA Coding Sequences Affect Ribosome Binding and Expression in Escherichia coli
journal, November 2006

Methods and compositions for directed cloning and subcloning using homologous recombination
patent, March 2002

ActRII Receptor Polypeptides, Methods And Compositions
patent-application, March 2006

Inhibiting Bacterial Fatty Acid Synthesis
journal, April 2006

Manipulating flux through plant metabolic pathways
journal, April 1998

The biological degradation of cellulose
journal, January 1994

Metabolically Engineered Organisms For Enhanced Production Of Oxaloacetate-derived Biochemicals
patent-application, May 2003

Engineering the pathway for succinate production
patent-application, March 2012

Beta-alanine synthesis in Escherichia coli.
journal, January 1980

Method for producing nucleic acid substances
patent, September 2001

Thin, flexible sanitary napkin
patent, April 1991

Dihydrolipoamide Dehydrogenase Mutation Alters the NADH Sensitivity of Pyruvate Dehydrogenase Complex of Escherichia coli K-12
journal, March 2008

Toward a science of metabolic engineering
journal, June 1991

Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis
journal, May 2003

Alanine 2, 3 Aminomutases
patent-application, January 2009

Reverse Beta Oxidation Pathway
patent-application, November 2013

Schizochytrium PKS Genes
patent-application, May 2003

Disposable diaper having density and basis weight profiled absorbent core
patent, August 1987

Light-directed, spatially addressable parallel chemical synthesis
journal, February 1991

Method for the production of &rgr;-Hydroxybenzoate in species of pseudomonas and agrobacterium
patent, July 2003

Characterization of Mutations Contributing to Sulfathiazole Resistance in Escherichia coli
journal, January 1998

Polymers for use in water treatment
patent, June 1977