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Title: Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids

Abstract

The invention provides miniaturized lateral flow chromatographic and lateral flow chromatographic microarray devices (LFM). The miniaturization of lateral flow nucleic acid detection achieved by the present invention offers reduced reagent use, femtomole sensitivity, excellent linear dynamic range, and rapid detection. Moreover, the small feature sizes of capture oligonucleotides renders the potential information capacity of the platform comparable to more traditional spotted fluorescence microarrays as well as improving sensitivity. The LFM devices exemplified herein enable analytes to be detected within 10 seconds from the time of sample introduction to the LFM device. Sample volumes may be as low as about 10 microliters, significantly reducing assay costs and ameliorating reagent storage logistics. Additionally, the miniaturization of lateral flow opens the door to highly multiplexed assays, allowing many proteins or nucleic acids to be detected in a single assay.

Inventors:
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1600229
Patent Number(s):
10,458,978
Application Number:
15/431,705
Assignee:
Triad National Security, LLC (Los Alamos, NM)
DOE Contract Number:  
AC52-06NA25396
Resource Type:
Patent
Resource Relation:
Patent File Date: 02/13/2017
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Cary, Robert B. Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids. United States: N. p., 2019. Web.
Cary, Robert B. Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids. United States.
Cary, Robert B. Tue . "Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids". United States. https://www.osti.gov/servlets/purl/1600229.
@article{osti_1600229,
title = {Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids},
author = {Cary, Robert B.},
abstractNote = {The invention provides miniaturized lateral flow chromatographic and lateral flow chromatographic microarray devices (LFM). The miniaturization of lateral flow nucleic acid detection achieved by the present invention offers reduced reagent use, femtomole sensitivity, excellent linear dynamic range, and rapid detection. Moreover, the small feature sizes of capture oligonucleotides renders the potential information capacity of the platform comparable to more traditional spotted fluorescence microarrays as well as improving sensitivity. The LFM devices exemplified herein enable analytes to be detected within 10 seconds from the time of sample introduction to the LFM device. Sample volumes may be as low as about 10 microliters, significantly reducing assay costs and ameliorating reagent storage logistics. Additionally, the miniaturization of lateral flow opens the door to highly multiplexed assays, allowing many proteins or nucleic acids to be detected in a single assay.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {10}
}

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Works referenced in this record:

Rapid screening assay methods and devices
patent, July 1999


High surface area substrates for microarrays and methods to make same
patent, March 2007


gravity-driven apparatus and method for control of microfluidic devices
patent, September 2007


Liquid transfer devices
patent, October 1994


Process for amplifying nucleic acid sequences
patent, July 1987


Process for amplifying, detecting, and/or cloning nucleic acid sequences
patent, January 1989


Solid supports for nucleic acid hybridization assays
patent, May 1996


Printed fluid transport devices
patent, April 1998


Process for amplifying, detecting, and/or-cloning nucleic acid sequences
patent, July 1987


Oscillating amplification reaction for nucleic acids
patent, August 2016


Capture and detection of target nucleic acid in dipstick assays
patent, March 2007


Oxygen sensing membranes and methods of making same
patent, February 2001


Reaction apparatus employing gravitational flow
patent, July 1993


Pumpless microfluidics
patent, June 2004


Electrically opened micro fluid valve
patent, January 2007


Generation and amplification of nucleic acids from ribonucleic acids
patent, October 2001


Virus detection method and materials
patent, May 1987


Method for detection of non-denatured nucleic acid fragments
patent, March 2000


Mesothelium antigen and methods and kits for targeting it
patent, July 2000


Chromatographic test strip for determining ligands or receptors
patent, October 1990


Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
patent, July 2001


Gravity-driven micropump
patent, May 2012


Test device and method for its use
patent, November 1980


Reversible flow chromatographic binding assay
patent, December 1999


Sequence-dependent gene sorting techniques
patent, October 2002


Isothermal strand displacement nucleic acid amplification
patent, April 2001


Capillary flow liquid transfer device having waste reception area
patent, April 1997