Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
Abstract
The invention provides miniaturized lateral flow chromatographic and lateral flow chromatographic microarray devices (LFM). The miniaturization of lateral flow nucleic acid detection achieved by the present invention offers reduced reagent use, femtomole sensitivity, excellent linear dynamic range, and rapid detection. Moreover, the small feature sizes of capture oligonucleotides renders the potential information capacity of the platform comparable to more traditional spotted fluorescence microarrays as well as improving sensitivity. The LFM devices exemplified herein enable analytes to be detected within 10 seconds from the time of sample introduction to the LFM device. Sample volumes may be as low as about 10 microliters, significantly reducing assay costs and ameliorating reagent storage logistics. Additionally, the miniaturization of lateral flow opens the door to highly multiplexed assays, allowing many proteins or nucleic acids to be detected in a single assay.
- Inventors:
- Issue Date:
- Research Org.:
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1600229
- Patent Number(s):
- 10458978
- Application Number:
- 15/431,705
- Assignee:
- Triad National Security, LLC (Los Alamos, NM)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- AC52-06NA25396
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 02/13/2017
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Cary, Robert B. Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids. United States: N. p., 2019.
Web.
Cary, Robert B. Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids. United States.
Cary, Robert B. Tue .
"Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids". United States. https://www.osti.gov/servlets/purl/1600229.
@article{osti_1600229,
title = {Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids},
author = {Cary, Robert B.},
abstractNote = {The invention provides miniaturized lateral flow chromatographic and lateral flow chromatographic microarray devices (LFM). The miniaturization of lateral flow nucleic acid detection achieved by the present invention offers reduced reagent use, femtomole sensitivity, excellent linear dynamic range, and rapid detection. Moreover, the small feature sizes of capture oligonucleotides renders the potential information capacity of the platform comparable to more traditional spotted fluorescence microarrays as well as improving sensitivity. The LFM devices exemplified herein enable analytes to be detected within 10 seconds from the time of sample introduction to the LFM device. Sample volumes may be as low as about 10 microliters, significantly reducing assay costs and ameliorating reagent storage logistics. Additionally, the miniaturization of lateral flow opens the door to highly multiplexed assays, allowing many proteins or nucleic acids to be detected in a single assay.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {10}
}
Works referenced in this record:
Rapid screening assay methods and devices
patent, July 1999
- Wang, Mark S.; Li, Limin
- US Patent Document 5,922,617
Enzymatic synthesis of repeat regions of oligonucleotides
patent, May 1996
- Hyman, Edward D.
- US Patent Document 5,516,664
Pharmaceutical composition containing a stabilised mRNA optimised for translation in its coding regions
patent-application, February 2005
- Von Der Mulbe, Florian; Hoerr, Ingmar; , Steve Pascolo
- US Patent Application 10/729830; 20050032730
High surface area substrates for microarrays and methods to make same
patent, March 2007
- Agrawal, Anoop; Cronin, John P.; Tonazzi, Juan
- US Patent Document 7,195,872
gravity-driven apparatus and method for control of microfluidic devices
patent, September 2007
- Yao, Nan-Kuang; Wu, Jhy-Wen
- US Patent Document 7,273,590
Method and apparatus for washing magnetically responsive particles
patent-application, September 2003
- Hoet, Rene; Hoogenboom, Henricus Renerus Jacobus Mattheus; Pieters, Henk
- US Patent Application 10/313822; 20030170686
Two step lateral flow assay methods and devices
patent-application, January 2007
- Rundstrom, Gerd; Matsson, Per
- US Patent Application 11/438882; 20070020768
Use of deoxyinosine containing primers to balance primer efficiency in the amplification of nucleic acid molecules
patent, November 1996
- Schuster, David M.; Rashtchian, Ayoub
- US Patent Document 5,578,467
Liquid transfer devices
patent, October 1994
- Bunce, Roger A.; Thorpe, Gary H.; Gibbons, John E.
- US Patent Document 5,354,538
Lateral flow methods and devices for detection of nucleic acid binding proteins
patent-application, January 2007
- Nilsen, Thor W.
- US Patent Application 11/181260; 20070015166
Flow through nucleic acid hybridisation uses thereof and a device thereof
patent, April 1998
- Tam, Joseph Wing
- US Patent Document 5,741,647
Chip-based isothermal amplification devices and methods
patent-application, November 2002
- Burns, Mark A.; Burke, David T.; Johnson, Brian N.
- US Patent Application 10/113238; 20020172969
Collection device for single step assay of oral fluids
patent-application, December 2002
- Mink, Ronald W.; Goldstein, Andrew S.; Bohannon, Robert C.
- US Patent Application 10/076596; 20020192839
Process for amplifying nucleic acid sequences
patent, July 1987
- Mullis, Kary B.
- US Patent Document 4,683,202
Process for amplifying, detecting, and/or cloning nucleic acid sequences
patent, January 1989
- Mullis, Kary B.; Erlich, Henry A.; Arnheim, Norman
- US Patent Document 4,800,159
Methods and compositions for amplification of RNA sequences
patent-application, January 2005
- Kurn, Nurith
- US Patent Application 10/934890; 20050014192
Diagnostic marker for tumor hypoxia and prognosis
patent-application, March 2003
- Giaccia, Amato J.; Le, Quynh Thu; Denko, Nicholas C.
- US Patent Application 10/175440; 20030044862
Use of photopolymerization for amplification and detection of a molecular recognition event
patent-application, December 2006
- Rowlen, Kathy L.; Birks, John W.; Bowman, Christopher
- US Patent Application 11/372485; 20060286570
Lateral flow assay device and method
patent-application, July 2006
- Cardy, Donald Leonard Nicholas; Allen, Gerard John
- US Patent Application 10/521111; 20060160078
Systems and methods for detection of analytes in biological fluids
patent-application, January 2003
- Kovalenko, Victor
- US Patent Application 10/138654; 20030003514
Nucleic acid detection system and method for detecting influenza
patent, March 2015
- Cai, Hong; Song, Jian
- US Patent Document 8,980,561
Method for extending the dynamic detection range of assay devices
patent-application, May 2005
- Song, Xuedong
- US Patent Application 10/719976; 20050112780
Nucleic acid amplification utilizing microfluidic devices
patent-application, January 2003
- Enzelberger, Markus M.; Hansen, Carl L.; Liu, JIan
- US Patent Application 10/118466; 20030008308
Microfluidic Cartridge
patent-application, May 2009
- Handique, Kalyan
- US Patent Application 12/239537; 20090130719
Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase
patent, April 2003
- O'Donnell, Michael
- US Patent Document 6,555,349
Method and test strip for determining an analyte
patent, January 2002
- Bausback, Jrg
- US Patent Document 6,335,205
Solid supports for nucleic acid hybridization assays
patent, May 1996
- Van Ness, Jeffrey; Petrie, III, Charles R.; Tabone, John C.
- US Patent Document 5,514,785
Printed fluid transport devices
patent, April 1998
- Alcock, Susan; White, Stephen; Turner, Anthony
- US Patent Document 5,736,188
Microfluidic devices comprising biochannels
patent-application, October 2003
- Blackburn, Gary
- US Patent Application 09/861171; 20030190608
Helicase-dependent amplification of nucleic acids
patent-application, July 2006
- Kong, Huimin; Vincent, Myriam; Xu, Yan
- US Patent Application 11/327175; 20060154286
Cloning and expression of T5 DNA polymerase reduced in 3'-to-5' exonuclease activity
patent, February 1998
- Chatterjee, Deb K.
- US Patent Document 5,716,819
Process for amplifying, detecting, and/or-cloning nucleic acid sequences
patent, July 1987
- Mullis, Kary B.; Erlich, Henry A.; Arnheim, Norman
- US Patent Document 4,683,195
Methods and compositions for amplification of RNA sequences
patent, August 2006
- Kurn, Nurith
- US Patent Document 7,094,536
Oscillating amplification reaction for nucleic acids
patent, August 2016
- Cai, Hong; Cobb, Nathan J.
- US Patent Document 9,428,781
Integrated biochip system for sample preparation and analysis
patent-application, June 2002
- Cheng, Jing; Wang, Xiaobo; Wu, Lei
- US Patent Application 09/973629; 20020076825
Asymmetrically branched polymer conjugates and microarray assays
patent-application, February 2006
- Yin, Ray; Qin, Dujie; Pan, Jing
- US Patent Application 10/993474; 20060041058
Lateral flow system for nucleic acid detection
patent-application, June 2004
- Gerdes, John C.; Mondesire, Roy R.; Hansen, Lara A.
- US Patent Application 10/413433; 20040110167
Capture and detection of target nucleic acid in dipstick assays
patent, March 2007
- Lee, Helen; Dineva, Magda Anastassova; Hazlewood, Shaun Christopher
- US Patent Document 7,186,508
Oxygen sensing membranes and methods of making same
patent, February 2001
- Berger, Joseph; Collins, Thomas C.; Slovacek, Rudolf E.
- US Patent Document 6,190,612
Amelioration of Heterophile Antibody Immunosensor Interference
patent-application, September 2010
- Campbell, John Lewis Emerson; Omakor, John Emegbero
- US Patent Application 12/411325; 20100248273
Reaction apparatus employing gravitational flow
patent, July 1993
- Andrews, Robert R.
- US Patent Document 5,225,163
Sample-efficient lateral flow immunoassay
patent-application, June 2006
- Kaylor, Rosann Marie; Wei, Ning; Chidebelu-Eze, Chibueze
- US Patent Application 11/012759; 20060127886
Micro-arrayed organization of transcription factor target genes
patent-application, April 2005
- Burgess Jr., Robert M.; Lunyak, Victoria; Noskin, Leonid
- US Patent Application 10/275845; 20050079492
Assay device for detecting the presence of an analyte involving sequential delivery of reagents through a liquid circuit
patent, November 2000
- Chandler, John Anthony
- US Patent Document 6,146,589
Diagnostic assays including multiplexed lateral flow immunoassays with quantum dots
patent-application, November 2005
- Lambert, James L.; Fisher, Anita M.
- US Patent Application 11/094115; 20050250141
Pumpless microfluidics
patent, June 2004
- Weigl, Bernhard H.; Williams, Clinton L.; Hayenga, Jon W.
- US Patent Document 6,743,399
Helicase dependent amplification of nucleic acids
patent-application, March 2004
- Kong, Huimin; Vincent, Myriam; Xu, Yan
- US Patent Application 10/665633; 20040058378
Transmission-based optical detection systems
patent-application, November 2005
- Cohen, David Samuel; Song, Xuedong; Knotts, Michael
- US Patent Application 11/022287; 20050243321
Nano-mechanic microsensors and methods for detecting target analytes
patent-application, February 2006
- Warthoe, Peter
- US Patent Application 11/134821; 20060024813
Electrically opened micro fluid valve
patent, January 2007
- Broyer, Patrick; Colin, Bruno; Roller, Denis
- US Patent Document 7,159,618
Displacement assay on a porous membrane
patent-application, March 2002
- Ligler, Frances S.; Kusterbeck, Anne W.; Rabbany, Sina Y.
- US Patent Application 09/907888; 20020028475
Nucleic acid analysis techniques
patent-application, April 2003
- Lockhart, David J.; Chee, Mark; Gunderson, Kevin
- US Patent Application 09/880727; 20030064364
Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control
patent, April 2018
- Cary, Robert B.
- US Patent Document 9,944,922
Generation and amplification of nucleic acids from ribonucleic acids
patent, October 2001
- Missel, Andreas; Loffert, Dirk; Kang, Jie
- US Patent Document 6,300,069
Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection
patent-application, December 2002
- Liisa, Marja; Wada, H. Garrett; Dubrow, Robert S.
- US Patent Application 10/142263; 20020179445
Nucleic acid detection system
patent-application, October 2005
- Jung, Jaean; Choi, Young Ho; Kim, Youngsun
- US Patent Application 11/102001; 20050227275
Method for detection of non-denatured nucleic acid fragments
patent, March 2000
- Ebersole, Richard C.; Hendrickson, Edwin R.; Payne, Mark
- US Patent Document 6,037,127
Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control
patent, December 2015
- Cary, Robert B.
- US Patent Document 9,207,236
Mesothelium antigen and methods and kits for targeting it
patent, July 2000
- Pastan, Ira H.; Chang, Kai
- US Patent Document 6,083,502
Assay devices having detection capabilities within the hook effect region
patent-application, November 2006
- Song, Xuedong; Christopher, Paul
- US Patent Application 11/119262; 20060246601
Chromatographic test strip for determining ligands or receptors
patent, October 1990
- Gordon, Julian; McMahon, Michael E.; Ching, Shanfun
- US Patent Document 4,960,691
Unitary biochip providing sample-in to results-out processing and methods of manufacture
patent, May 2016
- Selden, Richard; Tan, Eugene
- US Patent Document 9,354,199
Controlled flow assay device and method
patent-application, October 2006
- Ohman, Per Ove; Mendel-Hartvig, Ib
- US Patent Application 10/560214; 20060239859
Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
patent, July 2001
- Barbera-Guillem, Emilio; Nelson, M. Bud; Castro, Stephanie L.
- US Patent Document 6,261,779
Apparatus and method for calibration of a microarray scanning system
patent, October 2002
- Noblett, David Allan
- US Patent Document 6,471,916
Test device and method for its use
patent, November 1980
- Deutsch, Marshall E.; Mead, Louis W.
- US Patent Document 4,235,601
Method of adjusting the working range of a multi-analyte assay
patent-application, August 2006
- Dowd, Roger; Donahue, Jeffrey G.
- US Patent Application 11/221198; 20060177873
Reversible flow chromatographic binding assay
patent, December 1999
- Clark, Scott M.
- US Patent Document 6,007,999
Sequence-dependent gene sorting techniques
patent, October 2002
- Ulanovsky, Levy; Mugasimangalam, Raja C.; Einat, Paz
- US Patent Document 6,468,749
Isothermal strand displacement nucleic acid amplification
patent, April 2001
- Dattagupta, Nanibhushan; Stull, Paul D.; Spingola, Marc
- US Patent Document 6,214,587
Capillary flow liquid transfer device having waste reception area
patent, April 1997
- Bunce, Roger A.; Starsmore, Stephen J.; Thorpe, Gary H.
- US Patent Document 5,618,494