Tyrosine hydroxylase variants and methods of use thereof
Abstract
The present disclosure provides a variant tyrosine hydroxylase that provides for increased production of L-DOPA in a host cell that expresses the tyrosine hydroxylase. The present disclosure provides nucleic acids encoding the variant tyrosine hydroxylase, and host cells genetically modified with the nucleic acids. The present disclosure provides methods of making L-DOPA in a host cell. The present disclosure provides methods of making a benzylisoquinoline alkaloid (BIA), or a BIA precursor. The present disclosure provides methods of detecting L-DOPA level in a cell. The present disclosure provides methods of identifying tyrosine hydroxylase variants that provide for increased L-DOPA production; and methods of identifying gene products that provide for increased tyrosine production.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California, Oakland, CA (United States); Valorbec S.E.C., Montreal, QC (Canada)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1600224
- Patent Number(s):
- 10,457,918
- Application Number:
- 15/507,711
- Assignee:
- The Regents of the University of California (Oakland, CA); Valorbec S.E.C. (Montreal, CA)
- DOE Contract Number:
- SC0008084
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 09/24/2015
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Martin, Vincent J. J., Narcross, Lauren, Dueber, John E., DeLoache, William C., Russ, Zachary N., and Scrivens, P. James. Tyrosine hydroxylase variants and methods of use thereof. United States: N. p., 2019.
Web.
Martin, Vincent J. J., Narcross, Lauren, Dueber, John E., DeLoache, William C., Russ, Zachary N., & Scrivens, P. James. Tyrosine hydroxylase variants and methods of use thereof. United States.
Martin, Vincent J. J., Narcross, Lauren, Dueber, John E., DeLoache, William C., Russ, Zachary N., and Scrivens, P. James. Tue .
"Tyrosine hydroxylase variants and methods of use thereof". United States. https://www.osti.gov/servlets/purl/1600224.
@article{osti_1600224,
title = {Tyrosine hydroxylase variants and methods of use thereof},
author = {Martin, Vincent J. J. and Narcross, Lauren and Dueber, John E. and DeLoache, William C. and Russ, Zachary N. and Scrivens, P. James},
abstractNote = {The present disclosure provides a variant tyrosine hydroxylase that provides for increased production of L-DOPA in a host cell that expresses the tyrosine hydroxylase. The present disclosure provides nucleic acids encoding the variant tyrosine hydroxylase, and host cells genetically modified with the nucleic acids. The present disclosure provides methods of making L-DOPA in a host cell. The present disclosure provides methods of making a benzylisoquinoline alkaloid (BIA), or a BIA precursor. The present disclosure provides methods of detecting L-DOPA level in a cell. The present disclosure provides methods of identifying tyrosine hydroxylase variants that provide for increased L-DOPA production; and methods of identifying gene products that provide for increased tyrosine production.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {10}
}
Works referenced in this record:
Genetically modified tyrosine hydroxylase and uses thereof
patent, April 1994
- Goldstein, Menek; Wu, Jing; Filer, David
- US Patent Document 5,300,436