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Title: Tyrosine hydroxylase variants and methods of use thereof

Abstract

The present disclosure provides a variant tyrosine hydroxylase that provides for increased production of L-DOPA in a host cell that expresses the tyrosine hydroxylase. The present disclosure provides nucleic acids encoding the variant tyrosine hydroxylase, and host cells genetically modified with the nucleic acids. The present disclosure provides methods of making L-DOPA in a host cell. The present disclosure provides methods of making a benzylisoquinoline alkaloid (BIA), or a BIA precursor. The present disclosure provides methods of detecting L-DOPA level in a cell. The present disclosure provides methods of identifying tyrosine hydroxylase variants that provide for increased L-DOPA production; and methods of identifying gene products that provide for increased tyrosine production.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
Univ. of California, Oakland, CA (United States); Valorbec S.E.C., Montreal, QC (Canada)
Sponsoring Org.:
USDOE
OSTI Identifier:
1600224
Patent Number(s):
10,457,918
Application Number:
15/507,711
Assignee:
The Regents of the University of California (Oakland, CA); Valorbec S.E.C. (Montreal, CA)
DOE Contract Number:  
SC0008084
Resource Type:
Patent
Resource Relation:
Patent File Date: 09/24/2015
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Martin, Vincent J. J., Narcross, Lauren, Dueber, John E., DeLoache, William C., Russ, Zachary N., and Scrivens, P. James. Tyrosine hydroxylase variants and methods of use thereof. United States: N. p., 2019. Web.
Martin, Vincent J. J., Narcross, Lauren, Dueber, John E., DeLoache, William C., Russ, Zachary N., & Scrivens, P. James. Tyrosine hydroxylase variants and methods of use thereof. United States.
Martin, Vincent J. J., Narcross, Lauren, Dueber, John E., DeLoache, William C., Russ, Zachary N., and Scrivens, P. James. Tue . "Tyrosine hydroxylase variants and methods of use thereof". United States. https://www.osti.gov/servlets/purl/1600224.
@article{osti_1600224,
title = {Tyrosine hydroxylase variants and methods of use thereof},
author = {Martin, Vincent J. J. and Narcross, Lauren and Dueber, John E. and DeLoache, William C. and Russ, Zachary N. and Scrivens, P. James},
abstractNote = {The present disclosure provides a variant tyrosine hydroxylase that provides for increased production of L-DOPA in a host cell that expresses the tyrosine hydroxylase. The present disclosure provides nucleic acids encoding the variant tyrosine hydroxylase, and host cells genetically modified with the nucleic acids. The present disclosure provides methods of making L-DOPA in a host cell. The present disclosure provides methods of making a benzylisoquinoline alkaloid (BIA), or a BIA precursor. The present disclosure provides methods of detecting L-DOPA level in a cell. The present disclosure provides methods of identifying tyrosine hydroxylase variants that provide for increased L-DOPA production; and methods of identifying gene products that provide for increased tyrosine production.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {10}
}

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Works referenced in this record:

Genetically modified tyrosine hydroxylase and uses thereof
patent, April 1994