Site-specific dynamic nuclear polarization NMR agents
Abstract
Dynamic nuclear polarization (DNP) agents are provided for DNP nuclear magnetic resonance of analytes. The DNP agents can have the structure A-X-L-R, where A is none or an amphiphilic group; X is a coupling group capable of site-specific binding with the analyte or, when A is an amphiphilic group, capable of site-specific binding with the amphiphilic group; L is a bond or a linker group; and R is a poly-radical group. The poly-radical can be a di-radical, a tri-radical, a tetra-radical, or a combination thereof. Methods of NMR measurement of an analyte comprising an NMR-detectable nucleus are provided. The methods can include the steps of providing a frozen sample containing the analyte and a DNP agent; applying radiation having a frequency that excites electron spin transitions in the DNP agent at an intensity to polarize the NMR-detectable nucleus; and detecting a signal from nuclear spin transitions in the NMR-detectable nucleus.
- Inventors:
- Issue Date:
- Research Org.:
- North Carolina State Univ., Raleigh, NC (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1576300
- Patent Number(s):
- 10,414,729
- Application Number:
- 15/222,428
- Assignee:
- North Carolina State University (Raleigh, NC)
- DOE Contract Number:
- FG02-02ER15354
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2016 Jul 28
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Smirnov, Alexej I., and Voynov, Maxim Anatolyevich. Site-specific dynamic nuclear polarization NMR agents. United States: N. p., 2019.
Web.
Smirnov, Alexej I., & Voynov, Maxim Anatolyevich. Site-specific dynamic nuclear polarization NMR agents. United States.
Smirnov, Alexej I., and Voynov, Maxim Anatolyevich. Tue .
"Site-specific dynamic nuclear polarization NMR agents". United States. https://www.osti.gov/servlets/purl/1576300.
@article{osti_1576300,
title = {Site-specific dynamic nuclear polarization NMR agents},
author = {Smirnov, Alexej I. and Voynov, Maxim Anatolyevich},
abstractNote = {Dynamic nuclear polarization (DNP) agents are provided for DNP nuclear magnetic resonance of analytes. The DNP agents can have the structure A-X-L-R, where A is none or an amphiphilic group; X is a coupling group capable of site-specific binding with the analyte or, when A is an amphiphilic group, capable of site-specific binding with the amphiphilic group; L is a bond or a linker group; and R is a poly-radical group. The poly-radical can be a di-radical, a tri-radical, a tetra-radical, or a combination thereof. Methods of NMR measurement of an analyte comprising an NMR-detectable nucleus are provided. The methods can include the steps of providing a frozen sample containing the analyte and a DNP agent; applying radiation having a frequency that excites electron spin transitions in the DNP agent at an intensity to polarize the NMR-detectable nucleus; and detecting a signal from nuclear spin transitions in the NMR-detectable nucleus.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {9}
}