Metabolic flux measurement, imaging and microscopy

Title: Metabolic flux measurement, imaging and microscopy

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Abstract

Provided herein are methods for measuring molecular flux rates of molecules of interest in a tissue sample in spatially-organized manner and generating output (e.g., an image, a heat map, a contour map, a table or a database) representing the molecular flux rates of each spatially-defined location of the sample. Provided herein are also the output, as well as systems and computer-readable medium with computer-executable instructions for determining molecular flux rates of molecules of interest in the sample.

Inventors:: Bowen, Benjamin P.; Louie, Katherine B.; Northen, Trent R.; Hellerstein, Marc K.

Issue Date:: 2019-08-20

Research Org.:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1568748

Patent Number(s):: 10386371

Application Number:: 14/343,334

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G - PHYSICS G16 - INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS G16B - BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2570/00 - Omics, e.g. proteomics, glycomics or lipidomics
G01N33/58 - involving labelled substances
G01N2458/15 - Non-radioactive isotope labels, e.g. for detection by mass spectrometry

G - PHYSICS G16 - INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS G16B - BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
G16B20/00 - ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/6848 - {Methods of protein analysis involving mass spectrometry}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
Y10T436/24 - Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2800/7028 - Cancer

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DOE Contract Number:: AC02-05CH11231; CA112970

Resource Type:: Patent

Resource Relation:: Patent File Date: 09/07/2012

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats


                    Bowen, Benjamin P., Louie, Katherine B., Northen, Trent R., and Hellerstein, Marc K. Metabolic flux measurement, imaging and microscopy.  United States: N. p., 2019. 
        Web.

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                    Bowen, Benjamin P., Louie, Katherine B., Northen, Trent R., & Hellerstein, Marc K. Metabolic flux measurement, imaging and microscopy.  United States.

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                    Bowen, Benjamin P., Louie, Katherine B., Northen, Trent R., and Hellerstein, Marc K. Tue .  
        "Metabolic flux measurement, imaging and microscopy".  United States.  https://www.osti.gov/servlets/purl/1568748.

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@article{osti_1568748,

  title        = {Metabolic flux measurement, imaging and microscopy},

  author       = {Bowen, Benjamin P. and Louie, Katherine B. and Northen, Trent R. and Hellerstein, Marc K.},

  abstractNote = {Provided herein are methods for measuring molecular flux rates of molecules of interest in a tissue sample in spatially-organized manner and generating output (e.g., an image, a heat map, a contour map, a table or a database) representing the molecular flux rates of each spatially-defined location of the sample. Provided herein are also the output, as well as systems and computer-readable medium with computer-executable instructions for determining molecular flux rates of molecules of interest in the sample.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2019},

  month        = {8}

}

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