DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Metabolic flux measurement, imaging and microscopy

Abstract

Provided herein are methods for measuring molecular flux rates of molecules of interest in a tissue sample in spatially-organized manner and generating output (e.g., an image, a heat map, a contour map, a table or a database) representing the molecular flux rates of each spatially-defined location of the sample. Provided herein are also the output, as well as systems and computer-readable medium with computer-executable instructions for determining molecular flux rates of molecules of interest in the sample.

Inventors:
; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1568748
Patent Number(s):
10386371
Application Number:
14/343,334
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G - PHYSICS G16 - INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS G16B - BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
DOE Contract Number:  
AC02-05CH11231; CA112970
Resource Type:
Patent
Resource Relation:
Patent File Date: 09/07/2012
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Bowen, Benjamin P., Louie, Katherine B., Northen, Trent R., and Hellerstein, Marc K. Metabolic flux measurement, imaging and microscopy. United States: N. p., 2019. Web.
Bowen, Benjamin P., Louie, Katherine B., Northen, Trent R., & Hellerstein, Marc K. Metabolic flux measurement, imaging and microscopy. United States.
Bowen, Benjamin P., Louie, Katherine B., Northen, Trent R., and Hellerstein, Marc K. Tue . "Metabolic flux measurement, imaging and microscopy". United States. https://www.osti.gov/servlets/purl/1568748.
@article{osti_1568748,
title = {Metabolic flux measurement, imaging and microscopy},
author = {Bowen, Benjamin P. and Louie, Katherine B. and Northen, Trent R. and Hellerstein, Marc K.},
abstractNote = {Provided herein are methods for measuring molecular flux rates of molecules of interest in a tissue sample in spatially-organized manner and generating output (e.g., an image, a heat map, a contour map, a table or a database) representing the molecular flux rates of each spatially-defined location of the sample. Provided herein are also the output, as well as systems and computer-readable medium with computer-executable instructions for determining molecular flux rates of molecules of interest in the sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {8}
}

Works referenced in this record:

Microarray of Three-Dimensional Heteropolymer Microstructures and Method Therefor
patent-application, February 2009


Methods for Conducting Metabolic Analyses
patent-application, July 2005


Method of detecting malignant neoplasms
patent, December 1977


Method for Isolating and Measuring Proliferation of Long-Term Label Retaining Cells and Stem Cells
patent-application, December 2003


Multidimensional Mass Spectrometry of Serum and Cellular Lipids Directly from Biologic Extracts
patent-application, September 2004


Methods and Compositions for the Assessment of Polymer Assembly
patent-application, January 2005


Method of Enhancing the Efficiency of a Pharmaceutical Business
patent-application, May 2006


Method of Mass Spectrometry
patent-application, June 2005


Methods for Measuring Cholesterol Metabolism and Transport
patent-application, November 2006


Measurement of gluconeogenesis and intermediary metabolism using stable isotopes
patent, August 2007


Microstructure and Microdomain Microarrays, Methods of Making Same and Uses Thereof
patent-application, March 2010


Stable Isotope Metabolic Labeling for Analysis of Biopolymers
patent-application, January 2005


Estimation of Protein-Compound Interaction and Rational Design of Compound Library Based on Chemical Genomic Information
patent-application, April 2010


Ratiometric Test Strip and Method
patent-application, December 2006


Generation of Efficacy, Toxicity and Disease Signatures and Methods of Use Thereof
patent-application, June 2004


13C glucose breath test for the diagnosis of diabetic indications and monitoring glycemic control
patent, October 2002


Cell-Based High-Throughput Screening Methods
patent-application, December 2004


Method for measuring human cholesterol absorption using metabolically stable isotopes
patent, July 1995


Isotope and assay for glycolysis and the pentose phosphate pathway
patent, August 1995


Method for Determining Metabolic Flux
patent-application, October 2005


Methods of Mass Spectrometry
patent-application, May 2005


Measurement of Gluconeogenesis and Intermediary Metabolism Using Stable Isotopes
patent-application, August 2003


Inhibition of cellular uptake of cholesterol
patent, July 1999


Glycan Analysis Using Deuterated Glucose
patent-application, June 2006


Methods and compositions to lower plasma cholesterol levels
patent, May 2005


Intracellular Metabolic Flux Analysis Method Using Substrate Labeled with Isotope
patent-application, August 2005


Isotopic Labeling for the Measurement of Global Protein Levels and Turnover In Vivo
patent-application, September 2014


Method of Determining the Probability of a Therapeutic Response in Cancer Chemotherapy with Cardiac Glycoside
patent-application, December 2010


Method for preparing biologically active organic compound labelled with hydrogen isotope
patent, June 1991


13C glucose breath test for the diagnosis of diabetic indications and monitoring glycemic control
patent, October 2002


13C glucose breath test for the diagnosis of diabetic indications and monitoring glycemic control
patent, July 2003


Methods for Screening Cellular Proliferation Using Isotope Labels
patent-application, April 2003


Methods for determining gluconeogenesis, anapleurosis and pyruvate recycling
patent, December 2001


Fourier transform mass spectrometry of complex biological samples
patent, January 2004


Non-invasive evaluation of maldigestion and malaborption
patent, April 1996


Creatine Hydroxycitric Acids Salts and Methods for their Production and Use in Individuals
patent-application, August 2006


Labeling of Protein Samples
patent-application, June 2003


Diagnostic and diagnosis method for central nervous abnormality and phenylketonuria
patent, June 1999


Systems for differential ion mobility analysis
patent, June 2006


Metabolic Flux Measurement, Imaging and Microscopy
patent-application, November 2014


Methods for determining total body skeletal muscle mass
patent, August 2017


Methods for Screening Cellular Proliferation Using Isotope Labels
patent-application, January 2006


Method of measurement in biological systems
patent, May 1993


13C glucose breath test for the diagnosis of diabetic indications and monitoring glycemic control
patent, August 2003


Method of measurement in biological systems
patent, December 1994


Nanostructure-Initiator Mass Spectrometry
patent-application, June 2008


Methods and apparatus for isotopic analysis
patent, February 1995


Non-radioisotope tagging of fragments
patent, May 1994


Methods and apparatus for determining proportions of body materials
patent, January 2011


Diagnostic agent for liver function
patent, June 2000


Administration system for estradiol
patent, September 1997


Rapid quantitative analysis of proteins or protein function in complex mixtures
patent, December 2003


Methods for Determining Total Body Skeletal Muscle Mass
patent-application, December 2014


Dynamic Hepatic Recycling Glucose Tolerance Test
patent-application, October 2005


Stable isotope metabolic labeling for analysis of biopolymers
patent, November 2003


Diagnostic kit for detecting creatine levels
patent, May 2003


Mass spectrometric quantification of chemical mixture components
patent, December 2004


Relative rates of cytochrome p450 metabolism
patent, September 2003


Mass spectrometry data analysis techniques
patent, June 2005


Isotopically labelled DNA
patent, April 2006


Measuring tissue breakdown products in body fluids
patent, October 1994


Methods for Conducting Metabolic Analyses
patent-application, May 2004


Biochemical Methods for Measuring Metabolic Fitness of Tissues or Whole Organisms
patent-application, April 2004


Dual isotope assays
patent, June 1982


Breath test for assessing hepatic function
patent, October 1999


Method of Enhancing the Efficiency of a Pharmaceutical Business
patent-application, September 2003


Analysis of Conjugated Metabolites of Alcohol Consumption
patent-application, April 2009


Methods for conducting metabolic analyses
patent, February 2005


Intracellular Metabolic Flux Analysis Method Using Substrate Labeled with Isotope
patent-application, May 2006


Method and Apparatus for Monitoring and Quantitatively Evaluating Tumor Perfusion
patent-application, November 2003


Liver Function Test
patent-application, August 2004


Biochemical Methods for Measuring Metabolic Fitness of Tissues or Whole Organisms
patent-application, October 2007


hC1Q/TNF7 and Uses Thereof
patent-application, August 2005


Method for measuring muscle mass
patent, May 1997


Method for improving the accuracy of the semi-quantitative determination of analyte in fluid samples
patent, October 2001