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Title: Scar-less multi-part DNA assembly design automation

Abstract

The present invention provides a method of a method of designing an implementation of a DNA assembly. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding flanking homology sequences to each of the DNA oligos. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding optimized overhang sequences to each of the DNA oligos.

Inventors:
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1568675
Patent Number(s):
10,373,703
Application Number:
15/147,764
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 05/05/2016
Country of Publication:
United States
Language:
English

Citation Formats

Hillson, Nathan J. Scar-less multi-part DNA assembly design automation. United States: N. p., 2019. Web.
Hillson, Nathan J. Scar-less multi-part DNA assembly design automation. United States.
Hillson, Nathan J. Tue . "Scar-less multi-part DNA assembly design automation". United States. https://www.osti.gov/servlets/purl/1568675.
@article{osti_1568675,
title = {Scar-less multi-part DNA assembly design automation},
author = {Hillson, Nathan J.},
abstractNote = {The present invention provides a method of a method of designing an implementation of a DNA assembly. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding flanking homology sequences to each of the DNA oligos. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding optimized overhang sequences to each of the DNA oligos.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {8}
}

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Works referenced in this record:

Purified thermostable enzyme
patent, December 1989


Preparation of chimaeric antibodies using the recombinant PCR strategy
patent, January 1999


Selecting tag nucleic acids
patent, October 2002