Scar-less multi-part DNA assembly design automation

Title: Scar-less multi-part DNA assembly design automation

Abstract

The present invention provides a method of a method of designing an implementation of a DNA assembly. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding flanking homology sequences to each of the DNA oligos. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding optimized overhang sequences to each of the DNA oligos.

Inventors:: Hillson, Nathan J.

Issue Date:: 2019-08-06

Research Org.:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1568675

Patent Number(s):: 10,373,703

Application Number:: 15/147,764

Assignee:: The Regents of the University of California (Oakland, CA)

DOE Contract Number:: AC02-05CH11231

Resource Type:: Patent

Resource Relation:: Patent File Date: 05/05/2016

Country of Publication:: United States

Language:: English

Citation Formats


                    Hillson, Nathan J. Scar-less multi-part DNA assembly design automation.  United States: N. p., 2019. 
        Web.

Copy to clipboard


                    Hillson, Nathan J. Scar-less multi-part DNA assembly design automation.  United States.

Copy to clipboard


                    Hillson, Nathan J. Tue .  
        "Scar-less multi-part DNA assembly design automation".  United States.  https://www.osti.gov/servlets/purl/1568675.

Copy to clipboard


                    
@article{osti_1568675,

  title        = {Scar-less multi-part DNA assembly design automation},

  author       = {Hillson, Nathan J.},

  abstractNote = {The present invention provides a method of a method of designing an implementation of a DNA assembly. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding flanking homology sequences to each of the DNA oligos. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding optimized overhang sequences to each of the DNA oligos.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2019},

  month        = {8}

}

Copy to clipboard

Patent:

View Patent

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record: