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Title: Affinity-assisted protein modification and recycling

Abstract

Methods for preparing a protein conjugate having a defined number of conjugate groups are provided. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Methods also include recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.

Inventors:
; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1568667
Patent Number(s):
10370407
Application Number:
15/545,459
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08B - POLYSACCHARIDES
C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08H - DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
DOE Contract Number:  
AC02-05CH11231; FA9550-11-C-0028
Resource Type:
Patent
Resource Relation:
Patent File Date: 01/21/2016
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Kwant, Richard L., Francis, Matthew B., Rosen, Christian B., and Rao, Meera. Affinity-assisted protein modification and recycling. United States: N. p., 2019. Web.
Kwant, Richard L., Francis, Matthew B., Rosen, Christian B., & Rao, Meera. Affinity-assisted protein modification and recycling. United States.
Kwant, Richard L., Francis, Matthew B., Rosen, Christian B., and Rao, Meera. Tue . "Affinity-assisted protein modification and recycling". United States. https://www.osti.gov/servlets/purl/1568667.
@article{osti_1568667,
title = {Affinity-assisted protein modification and recycling},
author = {Kwant, Richard L. and Francis, Matthew B. and Rosen, Christian B. and Rao, Meera},
abstractNote = {Methods for preparing a protein conjugate having a defined number of conjugate groups are provided. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Methods also include recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {8}
}

Patent:

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