Affinity-assisted protein modification and recycling
Abstract
Methods for preparing a protein conjugate having a defined number of conjugate groups are provided. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Methods also include recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568667
- Patent Number(s):
- 10370407
- Application Number:
- 15/545,459
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08B - POLYSACCHARIDES
- DOE Contract Number:
- AC02-05CH11231; FA9550-11-C-0028
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 01/21/2016
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Kwant, Richard L., Francis, Matthew B., Rosen, Christian B., and Rao, Meera. Affinity-assisted protein modification and recycling. United States: N. p., 2019.
Web.
Kwant, Richard L., Francis, Matthew B., Rosen, Christian B., & Rao, Meera. Affinity-assisted protein modification and recycling. United States.
Kwant, Richard L., Francis, Matthew B., Rosen, Christian B., and Rao, Meera. Tue .
"Affinity-assisted protein modification and recycling". United States. https://www.osti.gov/servlets/purl/1568667.
@article{osti_1568667,
title = {Affinity-assisted protein modification and recycling},
author = {Kwant, Richard L. and Francis, Matthew B. and Rosen, Christian B. and Rao, Meera},
abstractNote = {Methods for preparing a protein conjugate having a defined number of conjugate groups are provided. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Methods also include recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {8}
}