Engineering cyclopropane fatty acid accumulation in plants
Abstract
Heterologous expression of E. coli cyclopropane synthase in genotypic and phenotypic fad2fae1 plants facilitates accumulation of cyclopropane fatty acids. Co-expression of Sterculia foetida transferases, including lysophosphatidic acid acyltransferase, diacylglycerol acyltransferase (DGAT), and Phospholipid Diacyl Glycerol Acyltransferase (PDAT), with E. coli cyclopropane synthase further enhances cyclopropane fatty acid accumulation in fad2fae1 plant seeds.
- Inventors:
- Issue Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568600
- Patent Number(s):
- 10351868
- Application Number:
- 14/914,532
- Assignee:
- Brookhaven Science Associates, LLC (Upton, NY)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC02-98CH10886
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 08/26/2014
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Shanklin, John, Yu, Xiao-Hong, and Prakash, Richa Rawat. Engineering cyclopropane fatty acid accumulation in plants. United States: N. p., 2019.
Web.
Shanklin, John, Yu, Xiao-Hong, & Prakash, Richa Rawat. Engineering cyclopropane fatty acid accumulation in plants. United States.
Shanklin, John, Yu, Xiao-Hong, and Prakash, Richa Rawat. Tue .
"Engineering cyclopropane fatty acid accumulation in plants". United States. https://www.osti.gov/servlets/purl/1568600.
@article{osti_1568600,
title = {Engineering cyclopropane fatty acid accumulation in plants},
author = {Shanklin, John and Yu, Xiao-Hong and Prakash, Richa Rawat},
abstractNote = {Heterologous expression of E. coli cyclopropane synthase in genotypic and phenotypic fad2fae1 plants facilitates accumulation of cyclopropane fatty acids. Co-expression of Sterculia foetida transferases, including lysophosphatidic acid acyltransferase, diacylglycerol acyltransferase (DGAT), and Phospholipid Diacyl Glycerol Acyltransferase (PDAT), with E. coli cyclopropane synthase further enhances cyclopropane fatty acid accumulation in fad2fae1 plant seeds.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {7}
}
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