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Title: Engineering cyclopropane fatty acid accumulation in plants

Abstract

Heterologous expression of E. coli cyclopropane synthase in genotypic and phenotypic fad2fae1 plants facilitates accumulation of cyclopropane fatty acids. Co-expression of Sterculia foetida transferases, including lysophosphatidic acid acyltransferase, diacylglycerol acyltransferase (DGAT), and Phospholipid Diacyl Glycerol Acyltransferase (PDAT), with E. coli cyclopropane synthase further enhances cyclopropane fatty acid accumulation in fad2fae1 plant seeds.

Inventors:
; ;
Issue Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1568600
Patent Number(s):
10351868
Application Number:
14/914,532
Assignee:
Brookhaven Science Associates, LLC (Upton, NY)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC02-98CH10886
Resource Type:
Patent
Resource Relation:
Patent File Date: 08/26/2014
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Shanklin, John, Yu, Xiao-Hong, and Prakash, Richa Rawat. Engineering cyclopropane fatty acid accumulation in plants. United States: N. p., 2019. Web.
Shanklin, John, Yu, Xiao-Hong, & Prakash, Richa Rawat. Engineering cyclopropane fatty acid accumulation in plants. United States.
Shanklin, John, Yu, Xiao-Hong, and Prakash, Richa Rawat. Tue . "Engineering cyclopropane fatty acid accumulation in plants". United States. https://www.osti.gov/servlets/purl/1568600.
@article{osti_1568600,
title = {Engineering cyclopropane fatty acid accumulation in plants},
author = {Shanklin, John and Yu, Xiao-Hong and Prakash, Richa Rawat},
abstractNote = {Heterologous expression of E. coli cyclopropane synthase in genotypic and phenotypic fad2fae1 plants facilitates accumulation of cyclopropane fatty acids. Co-expression of Sterculia foetida transferases, including lysophosphatidic acid acyltransferase, diacylglycerol acyltransferase (DGAT), and Phospholipid Diacyl Glycerol Acyltransferase (PDAT), with E. coli cyclopropane synthase further enhances cyclopropane fatty acid accumulation in fad2fae1 plant seeds.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {7}
}

Works referenced in this record:

Lysophosphatidic acid acetyltransferases
patent, February 2005


Enhancement of hydroxy fatty acid accumulation in oilseed plants
patent, January 2012


Lysophosphatidic acid acetyltransferases
patent, October 2009


Plant cyclopropane fatty acid synthase genes, proteins, and uses thereof
patent, November 2008


Process for the production of Δ5-unsaturated fatty acids in transgenic organisms
patent, May 2010


Plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
patent, December 2010


Lysophosphatidic acid acyltransferase genes
patent, February 2012


Plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
patent, January 2013


Cyclopropane fatty acid expression in plants
patent, August 1999