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Title: Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

Abstract

Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.

Inventors:
; ;
Issue Date:
Research Org.:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1568512
Patent Number(s):
10329587
Application Number:
14/903,728
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-02ER63445; P50 HG005550
Resource Type:
Patent
Resource Relation:
Patent File Date: 07/08/2014
Country of Publication:
United States
Language:
English

Citation Formats

Church, George M., Esvelt, Kevin M., and Mali, Prashant G. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. United States: N. p., 2019. Web.
Church, George M., Esvelt, Kevin M., & Mali, Prashant G. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. United States.
Church, George M., Esvelt, Kevin M., and Mali, Prashant G. Tue . "Orthogonal Cas9 proteins for RNA-guided gene regulation and editing". United States. https://www.osti.gov/servlets/purl/1568512.
@article{osti_1568512,
title = {Orthogonal Cas9 proteins for RNA-guided gene regulation and editing},
author = {Church, George M. and Esvelt, Kevin M. and Mali, Prashant G.},
abstractNote = {Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {6}
}

Works referenced in this record:

Methods of Generating Nucleic Acid Fragments
patent-application, September 2011


Methods And Compositions For Rna-Directed Target Dna Modification And For Rna-Directed Modulation Of Transcription
patent-application, March 2014


Endroibonuclease Compositions and Methods of Use Thereof
patent-application, May 2013


Methods and compositions for treating Huntington's disease
patent-application, September 2013


Novel DNA-binding Proteins And Uses Thereof
patent-application, December 2011


Visualization of RNA in living cells
patent, July 2003


Prokaryotic RNAi-Like System and Methods of Use
patent-application, August 2011


Target DNA interference with crRNA
patent-application, March 2010