Methods and apparatus for preparing aqueous specimens for electron microscopy using volatile surfactants
Abstract
This disclosure provides systems, methods, and apparatus related to the preparation of electron microscopy specimens. In one aspect, a method includes providing an electron microscopy grid. An aqueous suspension including an electron microscopy sample is deposited on the electron microscopy grid. A portion of the aqueous suspension is removed from the electron microscopy grid. A volatile surfactant is contacted to the aqueous suspension. A thickness of aqueous suspension is allowed to decrease over a period of time. The aqueous suspension is then frozen.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568428
- Patent Number(s):
- 10309881
- Application Number:
- 15/194,777
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC02-05CH11231; GM083039
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 06/28/2016
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Glaeser, Robert M., and Han, Bong-Gyoon. Methods and apparatus for preparing aqueous specimens for electron microscopy using volatile surfactants. United States: N. p., 2019.
Web.
Glaeser, Robert M., & Han, Bong-Gyoon. Methods and apparatus for preparing aqueous specimens for electron microscopy using volatile surfactants. United States.
Glaeser, Robert M., and Han, Bong-Gyoon. Tue .
"Methods and apparatus for preparing aqueous specimens for electron microscopy using volatile surfactants". United States. https://www.osti.gov/servlets/purl/1568428.
@article{osti_1568428,
title = {Methods and apparatus for preparing aqueous specimens for electron microscopy using volatile surfactants},
author = {Glaeser, Robert M. and Han, Bong-Gyoon},
abstractNote = {This disclosure provides systems, methods, and apparatus related to the preparation of electron microscopy specimens. In one aspect, a method includes providing an electron microscopy grid. An aqueous suspension including an electron microscopy sample is deposited on the electron microscopy grid. A portion of the aqueous suspension is removed from the electron microscopy grid. A volatile surfactant is contacted to the aqueous suspension. A thickness of aqueous suspension is allowed to decrease over a period of time. The aqueous suspension is then frozen.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {6}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Liquid Medium for Preventing Charge-up in Electron Microscope and Method of Observing Sample Using the Same
patent-application, July 2009
- Kuwabata, Susumu; Torimoto, Tsukasa
- US Patent Application 12/2223012; 20090173882
Transmission Electron Microscopy for Imaging Live Cells
patent-application, May 2012
- De Jonge, Niels
- US Patent Application 13/299241; 20120120226
System and method for dispensing liquids
patent-application, May 2003
- Kale, Samesh; Seward, George; Kim, Namyong
- US Patent Application 10/117720; 20030080143