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Title: Protein-based nanobubble x-ray imaging method and agent

Abstract

Systems, compositions, methods and kits employ protein shells, such as ferritin or viral capsid shells, herein called nanobubbles, to enhance X-ray images of cells or body tissue under certain x-ray imaging methods. The nanobubbles have sub-micron size such as about 10 nm, about 40, 60, or 100 nm and may be functionalized for effective delivery to or uptake by a target tissue, in vivo or a cell culture. The nanobubbles are hollow, having a central core which may be empty or contain a fluid, such that the shells effectively form long-lived bubbles in the imaged environment, and are of low electron density and have different scattering properties than the surrounding tissue. X-ray imaging by spatial frequency heterodyne imaging enhances visualization or detection of tissue regions bearing the shells. The protein shells may be further treated to assure biocompatibility and/or to resist undesired interactions with non-targeted tissue, such as scavenging by the liver, or attack by the immune system. For example the nanobubbles may be filled with a hydrophobic gas such as perfluoropropane (octafluoropropane) to maintain their shape and resist hydrophilic interactions and delay the degradation of the protein shells. The nanobubbles may also be coated or treated by a surface-functionalizationmore » processes to effectively target specific tissues or tumor types, allow parenteral delivery, and/or deliver drugs or other agents to the imaged sites—so that when visualized by X-ray scatter imaging the presence of the nanobubbles indicates the existence and extent of the tumor or diseased tissue.« less

Inventors:
; ; ;
Issue Date:
Research Org.:
Brown Univ., Providence, RI (United States); Montana State Univ., Bozeman, MT (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1568420
Patent Number(s):
10,307,527
Application Number:
14/692,075
Assignee:
Brown University (Providence, RI); Montana State University (Bozeman, MT)
DOE Contract Number:  
FG02-08ER15937; R01 EB012027
Resource Type:
Patent
Resource Relation:
Patent File Date: 04/21/2015
Country of Publication:
United States
Language:
English

Citation Formats

Rose-Petruck, Christoph, Douglas, Trevor, Rand, Danielle, and Uchida, Masaki. Protein-based nanobubble x-ray imaging method and agent. United States: N. p., 2019. Web.
Rose-Petruck, Christoph, Douglas, Trevor, Rand, Danielle, & Uchida, Masaki. Protein-based nanobubble x-ray imaging method and agent. United States.
Rose-Petruck, Christoph, Douglas, Trevor, Rand, Danielle, and Uchida, Masaki. Tue . "Protein-based nanobubble x-ray imaging method and agent". United States. https://www.osti.gov/servlets/purl/1568420.
@article{osti_1568420,
title = {Protein-based nanobubble x-ray imaging method and agent},
author = {Rose-Petruck, Christoph and Douglas, Trevor and Rand, Danielle and Uchida, Masaki},
abstractNote = {Systems, compositions, methods and kits employ protein shells, such as ferritin or viral capsid shells, herein called nanobubbles, to enhance X-ray images of cells or body tissue under certain x-ray imaging methods. The nanobubbles have sub-micron size such as about 10 nm, about 40, 60, or 100 nm and may be functionalized for effective delivery to or uptake by a target tissue, in vivo or a cell culture. The nanobubbles are hollow, having a central core which may be empty or contain a fluid, such that the shells effectively form long-lived bubbles in the imaged environment, and are of low electron density and have different scattering properties than the surrounding tissue. X-ray imaging by spatial frequency heterodyne imaging enhances visualization or detection of tissue regions bearing the shells. The protein shells may be further treated to assure biocompatibility and/or to resist undesired interactions with non-targeted tissue, such as scavenging by the liver, or attack by the immune system. For example the nanobubbles may be filled with a hydrophobic gas such as perfluoropropane (octafluoropropane) to maintain their shape and resist hydrophilic interactions and delay the degradation of the protein shells. The nanobubbles may also be coated or treated by a surface-functionalization processes to effectively target specific tissues or tumor types, allow parenteral delivery, and/or deliver drugs or other agents to the imaged sites—so that when visualized by X-ray scatter imaging the presence of the nanobubbles indicates the existence and extent of the tumor or diseased tissue.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {6}
}

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