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Title: Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use

Abstract

Microorganisms that co-consume glucose with non-glucose carbohydrates, such as xylose, and methods of using same. The microorganisms comprise modifications that reduce or ablate the activity of a phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) protein or modifications that reduce or ablate the activity of a phosphoglucose isomerase and a GntR. The PTS protein may be selected from an enzyme I (EI), an HPr, an FPr, and an enzyme IIGlc (EIIGlc). Additional modifications include reduction or ablation of the activity of a pyruvate formate lyase, a lactate dehydrogenase, and a fumarate reductase and inclusion of recombinant pyruvate decarboxylase and alcohol dehydrogenase genes. The microorganisms are particularly suited to co-consuming glucose and xylose in media containing these substrates and producing ethanol therefrom.

Inventors:
;
Issue Date:
Research Org.:
Wisconsin Alumni Research Foundation, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1568392
Patent Number(s):
10,301,653
Application Number:
14/791,590
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
DOE Contract Number:  
FC02-07ER64494
Resource Type:
Patent
Resource Relation:
Patent File Date: 07/06/2015
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Reed, Jennifer L., and Kim, Joonhoon. Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use. United States: N. p., 2019. Web.
Reed, Jennifer L., & Kim, Joonhoon. Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use. United States.
Reed, Jennifer L., and Kim, Joonhoon. Tue . "Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use". United States. https://www.osti.gov/servlets/purl/1568392.
@article{osti_1568392,
title = {Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use},
author = {Reed, Jennifer L. and Kim, Joonhoon},
abstractNote = {Microorganisms that co-consume glucose with non-glucose carbohydrates, such as xylose, and methods of using same. The microorganisms comprise modifications that reduce or ablate the activity of a phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) protein or modifications that reduce or ablate the activity of a phosphoglucose isomerase and a GntR. The PTS protein may be selected from an enzyme I (EI), an HPr, an FPr, and an enzyme IIGlc (EIIGlc). Additional modifications include reduction or ablation of the activity of a pyruvate formate lyase, a lactate dehydrogenase, and a fumarate reductase and inclusion of recombinant pyruvate decarboxylase and alcohol dehydrogenase genes. The microorganisms are particularly suited to co-consuming glucose and xylose in media containing these substrates and producing ethanol therefrom.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {5}
}

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Works referenced in this record:

L-malate production by metabolically engineered escherichia coli
patent, November 2015


Primary alcohol producing organisms
patent, February 2016


Escherichia coli containing mutated lpdA gene and application thereof
patent, March 2017


Pichia transformation
patent, May 1990