Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use
Abstract
Microorganisms that co-consume glucose with non-glucose carbohydrates, such as xylose, and methods of using same. The microorganisms comprise modifications that reduce or ablate the activity of a phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) protein or modifications that reduce or ablate the activity of a phosphoglucose isomerase and a GntR. The PTS protein may be selected from an enzyme I (EI), an HPr, an FPr, and an enzyme IIGlc (EIIGlc). Additional modifications include reduction or ablation of the activity of a pyruvate formate lyase, a lactate dehydrogenase, and a fumarate reductase and inclusion of recombinant pyruvate decarboxylase and alcohol dehydrogenase genes. The microorganisms are particularly suited to co-consuming glucose and xylose in media containing these substrates and producing ethanol therefrom.
- Inventors:
- Issue Date:
- Research Org.:
- Wisconsin Alumni Research Foundation, Madison, WI (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568392
- Patent Number(s):
- 10,301,653
- Application Number:
- 14/791,590
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- DOE Contract Number:
- FC02-07ER64494
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 07/06/2015
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Reed, Jennifer L., and Kim, Joonhoon. Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use. United States: N. p., 2019.
Web.
Reed, Jennifer L., & Kim, Joonhoon. Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use. United States.
Reed, Jennifer L., and Kim, Joonhoon. Tue .
"Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use". United States. https://www.osti.gov/servlets/purl/1568392.
@article{osti_1568392,
title = {Microorganisms that co-consume glucose with non-glucose carbohydrates and methods of use},
author = {Reed, Jennifer L. and Kim, Joonhoon},
abstractNote = {Microorganisms that co-consume glucose with non-glucose carbohydrates, such as xylose, and methods of using same. The microorganisms comprise modifications that reduce or ablate the activity of a phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) protein or modifications that reduce or ablate the activity of a phosphoglucose isomerase and a GntR. The PTS protein may be selected from an enzyme I (EI), an HPr, an FPr, and an enzyme IIGlc (EIIGlc). Additional modifications include reduction or ablation of the activity of a pyruvate formate lyase, a lactate dehydrogenase, and a fumarate reductase and inclusion of recombinant pyruvate decarboxylase and alcohol dehydrogenase genes. The microorganisms are particularly suited to co-consuming glucose and xylose in media containing these substrates and producing ethanol therefrom.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {5}
}
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