Microbial production of renewable glycolate

Stephanopoulos, Gregory; Li, Zheng-Jun; Pereira, Brian

Title: Microbial production of renewable glycolate

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Abstract

Some aspects provide engineered microbes for glycolate production. Methods for microbe engineering and culturing are also provided herein. Such engineered microbes exhibit greatly enhanced capabilities for glycolate production.

Inventors:: Stephanopoulos, Gregory; Li, Zheng-Jun; Pereira, Brian

Issue Date:: 2019-05-21

Research Org.:: Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1568363

Patent Number(s):: 10294481

Application Number:: 15/281,994

Assignee:: Massachusetts Institute of Technology (Cambridge, MA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y207/01047 - D-Ribulokinase

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/90 - Isomerases

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y401/02013 - Fructose-bisphosphate aldolase

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/70 - Vectors or expression systems specially adapted for E. coli

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y401/03001 - Isocitrate lyase

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P7/42 - Hydroxy-carboxylic acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/0006 - {acting on CH-OH groups as donors

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y101/01026 - Glyoxylate reductase

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/88 - Lyases

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y102/01021 - Glycolaldehyde dehydrogenase

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/0008 - {acting on the aldehyde or oxo group of donors
C12N15/52 - Genes encoding for enzymes or proenzymes

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y501/03 - acting on carbohydrates and derivatives

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/1205 - {Phosphotransferases with an alcohol group as acceptor

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DOE Contract Number:: SC0006698

Resource Type:: Patent

Resource Relation:: Patent File Date: 09/30/2016

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats


                    Stephanopoulos, Gregory, Li, Zheng-Jun, and Pereira, Brian. Microbial production of renewable glycolate.  United States: N. p., 2019. 
        Web.

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                    Stephanopoulos, Gregory, Li, Zheng-Jun, & Pereira, Brian. Microbial production of renewable glycolate.  United States.

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                    Stephanopoulos, Gregory, Li, Zheng-Jun, and Pereira, Brian. Tue .  
        "Microbial production of renewable glycolate".  United States.  https://www.osti.gov/servlets/purl/1568363.

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@article{osti_1568363,

  title        = {Microbial production of renewable glycolate},

  author       = {Stephanopoulos, Gregory and Li, Zheng-Jun and Pereira, Brian},

  abstractNote = {Some aspects provide engineered microbes for glycolate production. Methods for microbe engineering and culturing are also provided herein. Such engineered microbes exhibit greatly enhanced capabilities for glycolate production.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2019},

  month        = {5}

}

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