Title: Methods for generating barcoded combinatorial libraries

Abstract

Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

Inventors:

Gill, Ryan T.; Garst, Andrew; Lipscomb, Tanya Elizabeth Warnecke; Bassalo, Marcelo Colika; Zeitoun, Ramsey Ibrahim

Issue Date:

2019-05-21

Research Org.:

Univ. of Colorado, Denver, CO (United States); Inscripta, Inc., Boulder, CO (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1568362

Patent Number(s):

10294473

Application Number:

15/948,793

Assignee:

The Regents of the University of Colorado, a body corporate (Denver, CO); Inscripta, Inc. (Boulder, CO)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/1065 - {Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags}
C12N15/102 - {Mutagenizing nucleic acids}
C12N15/1079 - {Screening libraries by altering the phenotype or phenotypic trait of the host

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q2563/179 - the label being a nucleic acid

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/1082 - {Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors}
C12N15/11 - DNA or RNA fragments
C12N2310/20 - involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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DOE Contract Number:  

SC0008812

Resource Type:

Patent

Resource Relation:

Patent File Date: 04/09/2018

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES