Methods for generating barcoded combinatorial libraries
Abstract
Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Colorado, Denver, CO (United States); Inscripta, Inc., Boulder, CO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568362
- Patent Number(s):
- 10294473
- Application Number:
- 15/948,793
- Assignee:
- The Regents of the University of Colorado, a body corporate (Denver, CO); Inscripta, Inc. (Boulder, CO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- SC0008812
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 04/09/2018
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Gill, Ryan T., Garst, Andrew, Lipscomb, Tanya Elizabeth Warnecke, Bassalo, Marcelo Colika, and Zeitoun, Ramsey Ibrahim. Methods for generating barcoded combinatorial libraries. United States: N. p., 2019.
Web.
Gill, Ryan T., Garst, Andrew, Lipscomb, Tanya Elizabeth Warnecke, Bassalo, Marcelo Colika, & Zeitoun, Ramsey Ibrahim. Methods for generating barcoded combinatorial libraries. United States.
Gill, Ryan T., Garst, Andrew, Lipscomb, Tanya Elizabeth Warnecke, Bassalo, Marcelo Colika, and Zeitoun, Ramsey Ibrahim. Tue .
"Methods for generating barcoded combinatorial libraries". United States. https://www.osti.gov/servlets/purl/1568362.
@article{osti_1568362,
title = {Methods for generating barcoded combinatorial libraries},
author = {Gill, Ryan T. and Garst, Andrew and Lipscomb, Tanya Elizabeth Warnecke and Bassalo, Marcelo Colika and Zeitoun, Ramsey Ibrahim},
abstractNote = {Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {5}
}
Works referenced in this record:
Microbial Strain Improvement by a HTP Genomic Engineering Platform
patent-application, June 2017
- Serber, Zach; Dean, Jed; Manchester, Shawn
- US Patent Application 15/396230; 20170159045
CRISPR enzymes and systems
patent, October 2017
- Zhang, Feng; Zetsche, Bernd; Slaymaker, Ian
- US Patent Document 9,790,490
CRISPR/CAS Systems for Genomic Modification and Gene Modulation
patent-application, September 2014
- Wu, Fangting
- US Patent Application 14/216655; 20140273226
Multiplex automated genome engineering
patent, October 2013
- Church, George M.; Wang, Harris H.; Isaacs, Farren J.
- US Patent Document 8,569,041
Engineering of systems, methods and optimized guide compositions for sequence manipulation
patent, December 2014
- Zhang, Feng; Cong, Le; Hsu, Patrick
- US Patent Document 8,906,616
Methods for generating barcoded combinatorial libraries
patent, July 2018
- Gill, Ryan T.; Garst, Andrew; Lipscomb, Tanya Elizabeth
- US Patent Document 10,017,760
Methods And Compositions For Rna-Directed Target Dna Modification And For Rna-Directed Modulation Of Transcription
patent-application, March 2014
- Doudna, Jennifer A.; Jinek, Martin; Charpentier, Emmanuelle
- US Patent Application 13/842859; 20140068797
Nucleic acid-guided nucleases
patent, July 2018
- Gill, Ryan T.; Garst, Andrew; Lipscomb, Tanya Elizabeth
- US Patent Document 10,011,849
Saturation mutagenesis in directed evolution
patent, May 2003
- Short, Jay M.
- US Patent Document 6,562,594
Compositions And Methods Of Nucleic Acid-Targeting Nucleic Acids
patent-application, February 2017
- May, Andrew Paul; Haurwitz, Rachel E.; Doudna, Jennifer A.
- US Patent Application 15/344487; 20170051276
Nucleic acid-guided nucleases
patent, May 2018
- Gill, Ryan T.; Garst, Andrew; Lipscomb, Tanya Elizabeth
- US Patent Document 9,982,279
Multiplex automated genome engineering
patent, April 2012
- Church, George M.; Wang, Harris H.; Isaacs, Farren J.
- US Patent Document 8,153,432
CRISPR enabled multiplexed genome engineering
patent, May 2018
- Gill, Ryan T.; Garst, Andrew
- US Patent Document 9,982,278