Multiplexed linking PCR
Abstract
The invention provides compositions and methods for rapid assembly of one or more assembled polynucleotides from a plurality of component polynucleotides, as well as methods of designing polynucleotide primer and linker sets useful in the assembly methods of the invention.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Colorado, Denver, CO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568326
- Patent Number(s):
- 10287627
- Application Number:
- 15/116,300
- Assignee:
- The Regents of the University of Colorado (Denver, CO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- SC0008812
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 02/09/2015
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Gill, Ryan T., Zeitoun, Ramsey, and Garst, Andrew. Multiplexed linking PCR. United States: N. p., 2019.
Web.
Gill, Ryan T., Zeitoun, Ramsey, & Garst, Andrew. Multiplexed linking PCR. United States.
Gill, Ryan T., Zeitoun, Ramsey, and Garst, Andrew. Tue .
"Multiplexed linking PCR". United States. https://www.osti.gov/servlets/purl/1568326.
@article{osti_1568326,
title = {Multiplexed linking PCR},
author = {Gill, Ryan T. and Zeitoun, Ramsey and Garst, Andrew},
abstractNote = {The invention provides compositions and methods for rapid assembly of one or more assembled polynucleotides from a plurality of component polynucleotides, as well as methods of designing polynucleotide primer and linker sets useful in the assembly methods of the invention.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {5}
}
Works referenced in this record:
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- US Patent Document 4,683,202
Mixed library parallel gene mapping quantitative micro-array technique for genome-wide identification of trait conferring genes
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Compositions and methods for the assembly of polynucleotides
patent, October 2013
- Serber, Zach; Lowe, Raymond; Ubersax, Jeffrey A.
- US Patent Document 8,546,136
Method for gene splicing by overlap extension using the polymerase chain reaction
patent, June 1991
- Ho, Steffan N.; Horton, Robert M.
- US Patent Document 5,023,171
Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
patent, October 1990
- Mullis, Kary B.; Erlich, Henry A.; Gelfand, David
- US Patent Document 4,965,188
Efficient linking of nucleic acid segments
patent, March 2001
- Liu, Qiang
- US Patent Document 6,204,025