Multiplexed linking PCR

Title: Multiplexed linking PCR

Abstract

The invention provides compositions and methods for rapid assembly of one or more assembled polynucleotides from a plurality of component polynucleotides, as well as methods of designing polynucleotide primer and linker sets useful in the assembly methods of the invention.

Inventors:: Gill, Ryan T.; Zeitoun, Ramsey; Garst, Andrew

Issue Date:: 2019-05-14

Research Org.:: Univ. of Colorado, Denver, CO (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1568326

Patent Number(s):: 10287627

Application Number:: 15/116,300

Assignee:: The Regents of the University of Colorado (Denver, CO)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/6853 - using modified primers or templates
C12Q2537/143 - Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
C12Q2537/101 - Homogeneous assay format, e.g. one pot reaction

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q2525/161 - incorporating target specific and non-target specific sites
C12Q2525/155 - incorporating/generating a new priming site

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y207/07007 - DNA-directed DNA polymerase

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DOE Contract Number:: SC0008812

Resource Type:: Patent

Resource Relation:: Patent File Date: 02/09/2015

Country of Publication:: United States

Language:: English

Citation Formats


                    Gill, Ryan T., Zeitoun, Ramsey, and Garst, Andrew. Multiplexed linking PCR.  United States: N. p., 2019. 
        Web.

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                    Gill, Ryan T., Zeitoun, Ramsey, & Garst, Andrew. Multiplexed linking PCR.  United States.

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                    Gill, Ryan T., Zeitoun, Ramsey, and Garst, Andrew. Tue .  
        "Multiplexed linking PCR".  United States.  https://www.osti.gov/servlets/purl/1568326.

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@article{osti_1568326,

  title        = {Multiplexed linking PCR},

  author       = {Gill, Ryan T. and Zeitoun, Ramsey and Garst, Andrew},

  abstractNote = {The invention provides compositions and methods for rapid assembly of one or more assembled polynucleotides from a plurality of component polynucleotides, as well as methods of designing polynucleotide primer and linker sets useful in the assembly methods of the invention.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2019},

  month        = {5}

}

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Works referenced in this record:

Process for amplifying nucleic acid sequences
patent, July 1987

Mullis, Kary B.
US Patent Document 4,683,202
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4683202

Mixed library parallel gene mapping quantitative micro-array technique for genome-wide identification of trait conferring genes
patent, June 2013

Ryan T., Gill; Lynch, Michael D.; Lipscomb, Tanya
US Patent Document 8,467,975
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=8467975

Compositions and methods for the assembly of polynucleotides
patent, October 2013

Serber, Zach; Lowe, Raymond; Ubersax, Jeffrey A.
US Patent Document 8,546,136
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=8546136

Method for gene splicing by overlap extension using the polymerase chain reaction
patent, June 1991

Ho, Steffan N.; Horton, Robert M.
US Patent Document 5,023,171
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5023171

Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
patent, October 1990

Mullis, Kary B.; Erlich, Henry A.; Gelfand, David
US Patent Document 4,965,188
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4965188

Efficient linking of nucleic acid segments
patent, March 2001

Liu, Qiang
US Patent Document 6,204,025
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6204025

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Similar Records

Title: Multiplexed linking PCR

Abstract

Citation Formats

Process for amplifying nucleic acid sequences patent, July 1987

Mixed library parallel gene mapping quantitative micro-array technique for genome-wide identification of trait conferring genes patent, June 2013

Compositions and methods for the assembly of polynucleotides patent, October 2013

Method for gene splicing by overlap extension using the polymerase chain reaction patent, June 1991

Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme patent, October 1990

Efficient linking of nucleic acid segments patent, March 2001

Process for amplifying nucleic acid sequences
patent, July 1987

Mixed library parallel gene mapping quantitative micro-array technique for genome-wide identification of trait conferring genes
patent, June 2013

Compositions and methods for the assembly of polynucleotides
patent, October 2013

Method for gene splicing by overlap extension using the polymerase chain reaction
patent, June 1991

Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
patent, October 1990

Efficient linking of nucleic acid segments
patent, March 2001