Engineering the pathway for succinate production
Abstract
This invention relates to biocatalysts for the efficient production of succinic acid and/or other products from renewable biological feedstocks. The biocatalysts have a very high efficiency for the growth-coupled production of succinic acid and/or other products from carbohydrate feed stocks as a result of both genetic manipulation and metabolic evolution. More specifically, certain biocatalysts of the present invention produce succinic acid at high titers and yields in mineral salts media during simple pH-controlled batch fermentation without the addition of any exogenous genetic material. The genetic manipulations of the present invention are concerned with energy-conserving strategies coupled with the elimination of alternative routes for NADH oxidation other than the routes for succinic acid production. The biocatalysts contain glucose-repressed gluconeogenic phosphoenolpyruvate carboxykinase (pck) derepressed by genetic modifications and a genetically-inactivated phosphotransferase system. In terms of succinic acid production efficiency, the biocatalysts of the present invention are functionally equivalent to succinate producing rumen bacteria such as Actinobacillus succinogens and Mannheimia succiniproducens, with one difference: that the biocatalysts are able to achieve this high level of succinic acid production in a minimal salt medium with carbohydrate source, as opposed to the requirement for a rich medium for succinic acid production by rumen bacteria.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568325
- Patent Number(s):
- 10287611
- Application Number:
- 14/705,011
- Assignee:
- University of Florida Research Foundation, Inc. (Gainesville, FL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG02-96ER20222; FG36-04GO14019
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 05/06/2015
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Zhang, Xueli, Jantama, Kaemwich, Moore, Jonathan C., Jarboe, Laura R., Shanmugam, Keelnatham T., and Ingram, Lonnie O'Neal. Engineering the pathway for succinate production. United States: N. p., 2019.
Web.
Zhang, Xueli, Jantama, Kaemwich, Moore, Jonathan C., Jarboe, Laura R., Shanmugam, Keelnatham T., & Ingram, Lonnie O'Neal. Engineering the pathway for succinate production. United States.
Zhang, Xueli, Jantama, Kaemwich, Moore, Jonathan C., Jarboe, Laura R., Shanmugam, Keelnatham T., and Ingram, Lonnie O'Neal. Tue .
"Engineering the pathway for succinate production". United States. https://www.osti.gov/servlets/purl/1568325.
@article{osti_1568325,
title = {Engineering the pathway for succinate production},
author = {Zhang, Xueli and Jantama, Kaemwich and Moore, Jonathan C. and Jarboe, Laura R. and Shanmugam, Keelnatham T. and Ingram, Lonnie O'Neal},
abstractNote = {This invention relates to biocatalysts for the efficient production of succinic acid and/or other products from renewable biological feedstocks. The biocatalysts have a very high efficiency for the growth-coupled production of succinic acid and/or other products from carbohydrate feed stocks as a result of both genetic manipulation and metabolic evolution. More specifically, certain biocatalysts of the present invention produce succinic acid at high titers and yields in mineral salts media during simple pH-controlled batch fermentation without the addition of any exogenous genetic material. The genetic manipulations of the present invention are concerned with energy-conserving strategies coupled with the elimination of alternative routes for NADH oxidation other than the routes for succinic acid production. The biocatalysts contain glucose-repressed gluconeogenic phosphoenolpyruvate carboxykinase (pck) derepressed by genetic modifications and a genetically-inactivated phosphotransferase system. In terms of succinic acid production efficiency, the biocatalysts of the present invention are functionally equivalent to succinate producing rumen bacteria such as Actinobacillus succinogens and Mannheimia succiniproducens, with one difference: that the biocatalysts are able to achieve this high level of succinic acid production in a minimal salt medium with carbohydrate source, as opposed to the requirement for a rich medium for succinic acid production by rumen bacteria.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {5}
}
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Works referenced in this record:
Ethanol production using engineered mutant E. coli
patent, July 1991
- Ingram, Lonnie O.; Clark, David P.
- US Patent Document 5,028,539
Materials and methods for the efficient production of acetate and other products
patent, July 2011
- Causey, Thomas B.; O'Neal Ingram, Lonnie; Shanmugam, Keelnatham T.
- US Patent Document 7,977,075
Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis
patent, November 1992
- Ingram, Lonnie O'Neal; Conway, Tyrrell
- US Patent Document 5,162,516
Method for construction of bacterial strains with increased succinic acid production
patent, December 2000
- Donnelly, Mark; Sanville-Millard, Cynthia; Chatterjee, Ranjini
- US Patent Document 6,159,738
Engineering of Thermotolerant Bacillus Coagulans for Production of D(-)-Lactic Acid
patent-application, May 2012
- Wang, Qingzhao; Shanmugam, Keelnatham T.; Ingram, Lonnie O.
- US Patent Application 13/301836; 20120129231
Evolved micro-organisms for the production of 1,2-propanediol
patent-application, March 2007
- Meynial-Salles, Isabelle; Gonzalez, Benjamin; Soucaille, Philippe
- US Patent Application 10/585040; 20070072279
Materials and methods for the efficient production of xylitol
patent-application, March 2007
- Cirino, Patrick Carmen; Ingram, Lonnie O'Neal
- US Patent Application 11/523403; 20070072280
Materials and Methods for Efficient Succinate and Malate Production
patent-application, July 2010
- Jantama, Kaemwich; Haupt, Mark John; Zhang, Xueli
- US Patent Application 12/529826; 20100184171
Materials and methods for efficient lactic acid production
patent-application, February 2007
- Zhou, Shengde; Ingram, Lonnie O'Neal; Shanmugam, Keelnatham T.
- US Patent Application 11/501137; 20070037265
Mutant E. coli strain with increased succinic acid production
patent, May 2007
- Ka-Yiu, San; Bennett, George N.; Sanchez, Ailen
- US Patent Document 7,223,567
Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
patent, March 1991
- Ingram, Lonnie O'Neal; Conway, Tyrrell; Alterthum, Flavio
- US Patent Document 5,000,000
Method for high-density microarray medicated gene expression profiling
patent, August 2003
- Larossa, Robert A.; Wei, Yan
- US Patent Document 6,607,885
Recombination activating gene deficient mouse
patent, December 1996
- Alt, Frederick W.; Shinkai, Yoichi
- US Patent Document 5,583,278
Materials and methods for efficient lactic acid production
patent, December 2009
- Zhou, Shengde; Ingram, Lonnie O'Neal; Shanmugam, Keelnatham T.
- US Patent Document 7,629,162
Efficient method of preventing growth of microbial genetic transformant after transformation
patent, December 2006
- Sandal, Indra; Bhattacharya, Amita; Gulati, Ashu
- US Patent Document 7,145,058
Homo-Succinic Acid Producing Microorganism Variant and Process for Preparing Succinic Acid Using the Same
patent-application, June 2010
- Lee, Sang Yup; Kim, Ji Mahn; Lee, Jeong Wook
- US Patent Application 12/668868; 20100159544
Process for making succinic acid, microorganisms for use in the process and methods of obtaining the microorganisms
patent, March 1998
- Guettler, Michael V.; Jain, Mahendra K.; Soni, Bhupendra Kumar
- US Patent Document 5,723,322
Process for the fermentative preparation of D-pantothenic acid using coryneform bacteria
patent, June 2005
- Dusch, Nicole; Thomas, Hermann; Thierbach, Georg
- US Patent Document 6,911,329
Materials and methods for the production of D-phenylalanine
patent, March 2002
- Fotheringham, Ian; Taylor, Paul; Yoshida, Roberta K.
- US Patent Document 6,358,714
Materials and methods for the efficient production of acetate and other products
patent-application, August 2004
- Causey, Thomas B.; Ingram, Lonnie O'Neal; Zhou, Shengde
- US Patent Application 10/703812; 20040152159
Production of chemicals from lignocellulose, biomass or sugars
patent, August 2006
- Shanmugam, Keelnatham T.; Ingram, Lonnie O'Neal; Patel, Milind A.
- US Patent Document 7,098,009
Method for the production of dicarboxylic acids
patent, February 1999
- Nghiem, Nhuan P.; Donnelly, Mark; Millard, Cynthia S.
- US Patent Document 5,869,301
Aerobic succinate production in bacteria
patent-application, August 2005
- San, Ka-Yiu; Bennett, George N.; Lin, Henry
- US Patent Application 10/987511; 20050170482
Materials and Methods for the Efficient Production of Acetate and Other Products
patent-application, June 2009
- Causey, Thomas B.; Ingram, Lonnie O'neal; Zhou, Shengde
- US Patent Application 12/235074; 20090148914