Host cells and methods for producing isopentenol from mevalonate
Abstract
The present invention provides for a genetically modified host cell capable of producing isopentenol and/or 3-methyl-3-butenol, comprising (a) an increased expression of phosphomevalonate decarboxylase (PMD) (b) an increased expression of a phosphatase capable of converting isopentenol into 3-methyl-3-butenol, (c) optionally the genetically modified host cell does not express, or has a decreased expression of one or more of NudB, phosphomevalonate kinase (PMK), and/or PMD, and (d) optionally one or more further enzymes capable of converting isopentenol and/or 3-methyl-3-butenol into a third compound, such as isoprene.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1568260
- Patent Number(s):
- 10273506
- Application Number:
- 15/682,325
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B60 - VEHICLES IN GENERAL B60K - ARRANGEMENT OR MOUNTING OF PROPULSION UNITS OR OF TRANSMISSIONS IN VEHICLES
B - PERFORMING OPERATIONS B65 - CONVEYING B65G - TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 08/21/2017
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Lee, Taek Soon, and Kang, Aram. Host cells and methods for producing isopentenol from mevalonate. United States: N. p., 2019.
Web.
Lee, Taek Soon, & Kang, Aram. Host cells and methods for producing isopentenol from mevalonate. United States.
Lee, Taek Soon, and Kang, Aram. Tue .
"Host cells and methods for producing isopentenol from mevalonate". United States. https://www.osti.gov/servlets/purl/1568260.
@article{osti_1568260,
title = {Host cells and methods for producing isopentenol from mevalonate},
author = {Lee, Taek Soon and Kang, Aram},
abstractNote = {The present invention provides for a genetically modified host cell capable of producing isopentenol and/or 3-methyl-3-butenol, comprising (a) an increased expression of phosphomevalonate decarboxylase (PMD) (b) an increased expression of a phosphatase capable of converting isopentenol into 3-methyl-3-butenol, (c) optionally the genetically modified host cell does not express, or has a decreased expression of one or more of NudB, phosphomevalonate kinase (PMK), and/or PMD, and (d) optionally one or more further enzymes capable of converting isopentenol and/or 3-methyl-3-butenol into a third compound, such as isoprene.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {4}
}
Works referenced in this record:
Utilization of Phosphoketolase in the Production of Mevalonate, Isoprenoid Precursors, and Isoprene
patent-application, April 2013
- Beck, Zachary Q.; Eliot, Andrew C.; Peres, Caroline M.
- US Patent Application 13/646562; 20130089906