Cyanochrome fluorophores

Ulijasz, Andrew T.; Vierstra, Richard D.

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Title: Cyanochrome fluorophores

Abstract

Genetically-engineered cyanochrome fluorophore molecules (fluorophores) with increased fluorescence and with absorbing fluorescence in the blue and green (blue/green) portion of the light spectrum are provided. These fluorophores are derived from the domains of phytochromes, and in particular cyanobacterial phytochromes. Methods for generating these fluorophores and various applications of these fluorophores are also provided.

Inventors:: Ulijasz, Andrew T.; Vierstra, Richard D.

Issue Date:: 2014-09-02

Research Org.:: Wisconsin Alumni Research Foundation, Madison, WI (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1532115

Patent Number(s):: 8822162

Application Number:: 12/789,112

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Classifications (CPCs):: A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A61K49/0045 - {the fluorescent agent being a peptide or protein used for imaging or diagnosis in vivo}

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K14/195 - from bacteria

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/02 - involving viable microorganisms

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/5035 - {on sub-cellular localization}
G01N33/542 - with steric inhibition or signal modification, e.g. fluorescent quenching
G01N33/582 - {with fluorescent label}

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DOE Contract Number:: FG02-88ER13968

Resource Type:: Patent

Resource Relation:: Patent File Date: 2010-05-27

Country of Publication:: United States

Language:: English

Citation Formats


                    Ulijasz, Andrew T., and Vierstra, Richard D. Cyanochrome fluorophores.  United States: N. p., 2014. 
        Web.

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                    Ulijasz, Andrew T., & Vierstra, Richard D. Cyanochrome fluorophores.  United States.

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                    Ulijasz, Andrew T., and Vierstra, Richard D. Tue .  
        "Cyanochrome fluorophores".  United States.  https://www.osti.gov/servlets/purl/1532115.

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@article{osti_1532115,

  title        = {Cyanochrome fluorophores},

  author       = {Ulijasz, Andrew T. and Vierstra, Richard D.},

  abstractNote = {Genetically-engineered cyanochrome fluorophore molecules (fluorophores) with increased fluorescence and with absorbing fluorescence in the blue and green (blue/green) portion of the light spectrum are provided. These fluorophores are derived from the domains of phytochromes, and in particular cyanobacterial phytochromes. Methods for generating these fluorophores and various applications of these fluorophores are also provided.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2014},

  month        = {9}

}

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Works referenced in this record:

Phytochrome-Based Fluorophores
patent-application, March 2009

Ulijasz, Andrew T.; Zhang, Junrui; Forest, Katrina T.
US Patent Application 12/167222; 20090061444
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090061444

Phytofluors as fluorescent labels
patent, April 2000

Lagarias, John Clark; Murphy, John Thomas
US Patent Document 6,046,014
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6046014

Uses of green-fluorescent protein
patent, February 1996

Chalfie, Martin; Prasher, Douglas
US Patent Document 5,491,084
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5491084

Conjugate heat shock protein-binding peptides
patent-application, July 2003

Rothman, James E.; Mayhew, Mark; Hoe, Mee H.
US Patent Application 10/052578; 20030134787
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030134787

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