Isothermal amplification of DNA

Tabor, Stanley; Richardson, Charles C.

Title: Isothermal amplification of DNA

Abstract

A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.

Inventors:: Tabor, Stanley; Richardson, Charles C.

Issue Date:: 2014-04-29

Research Org.:: Harvard Univ., Cambridge, MA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1532093

Patent Number(s):: 8709724

Application Number:: 10/813,693

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q2522/101 - Single or double stranded nucleic acid binding proteins
C12Q2523/313 - Irradiation, e.g. UV irradiation
C12Q2521/501 - Ligase
C12Q2521/525 - Phosphatase
C12Q2527/101 - Temperature
C12Q1/6844 - Nucleic acid amplification reactions
C12Q2521/513 - Winding/unwinding enzyme, e.g. helicase

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DOE Contract Number:: FG02-96ER62251

Resource Type:: Patent

Resource Relation:: Patent File Date: 2003-11-07

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Tabor, Stanley, and Richardson, Charles C. Isothermal amplification of DNA.  United States: N. p., 2014. 
        Web.

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                    Tabor, Stanley, & Richardson, Charles C. Isothermal amplification of DNA.  United States.

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                    Tabor, Stanley, and Richardson, Charles C. Tue .  
        "Isothermal amplification of DNA".  United States.  https://www.osti.gov/servlets/purl/1532093.

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@article{osti_1532093,

  title        = {Isothermal amplification of DNA},

  author       = {Tabor, Stanley and Richardson, Charles C.},

  abstractNote = {A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2014},

  month        = {4}

}

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Works referenced in this record:

T7 DNA polymerase
patent, August 1990

Tabor, Stanley; Richardson, Charles C.
US Patent Document 4,946,786
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4946786

Promoter ligation activated transcription amplification of nucleic acid sequences
patent, March 1993

Berninger, Mark S.; Schuster, David M.; Rashtchian, Ayoub
US Patent Document 5,194,370
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5194370

Polymerase compositions and uses thereof
patent, September 1996

Sorge, Joseph A.; Mullinax, Rebecca Lynn
US Patent Document 5,556,772
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5556772

T7 DNA polymerase
patent, July 1990

Tabor, Stanley; Richardson, Charles C.
US Patent Document 4,942,130
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4942130

Works referencing / citing this record:

Nucleic acid amplification
patent, July 2018

Patel, Pranav
US Patent Document 10,017,809
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10017809

Nucleic acid amplification
patent, November 2018

Belhocine, Kamila; Lee, Josephine; Patel, Pranav
US Patent Document 10,131,939
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10131939

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Title: Isothermal amplification of DNA

Abstract

Citation Formats

T7 DNA polymerase patent, August 1990

Promoter ligation activated transcription amplification of nucleic acid sequences patent, March 1993

Polymerase compositions and uses thereof patent, September 1996

T7 DNA polymerase patent, July 1990

Nucleic acid amplification patent, July 2018

Nucleic acid amplification patent, November 2018

T7 DNA polymerase
patent, August 1990

Promoter ligation activated transcription amplification of nucleic acid sequences
patent, March 1993

Polymerase compositions and uses thereof
patent, September 1996

T7 DNA polymerase
patent, July 1990

Nucleic acid amplification
patent, July 2018

Nucleic acid amplification
patent, November 2018