Isothermal amplification of DNA

Title: Isothermal amplification of DNA

Abstract

A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.

Inventors:: Tabor, Stanley; Richardson, Charles C.

Issue Date:: 2014-04-29

Research Org.:: Harvard College, Cambridge, MA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1532093

Patent Number(s):: 8,709,724

Application Number:: 10/813,693

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

DOE Contract Number:: FG02-96ER62251

Resource Type:: Patent

Resource Relation:: Patent File Date: 2003-11-07

Country of Publication:: United States

Language:: English

Citation Formats


                    Tabor, Stanley, and Richardson, Charles C. Isothermal amplification of DNA.  United States: N. p., 2014. 
        Web.

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                    Tabor, Stanley, & Richardson, Charles C. Isothermal amplification of DNA.  United States.

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                    Tabor, Stanley, and Richardson, Charles C. Tue .  
        "Isothermal amplification of DNA".  United States.  https://www.osti.gov/servlets/purl/1532093.

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@article{osti_1532093,

  title        = {Isothermal amplification of DNA},

  author       = {Tabor, Stanley and Richardson, Charles C.},

  abstractNote = {A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2014},

  month        = {4}

}

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Works referenced in this record:

T7 DNA polymerase
patent, August 1990

Tabor, Stanley; Richardson, Charles C.
US Patent Document 4,946,786
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4946786

Promoter ligation activated transcription amplification of nucleic acid sequences
patent, March 1993

Berninger, Mark S.; Schuster, David M.; Rashtchian, Ayoub
US Patent Document 5,194,370
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5194370

Polymerase compositions and uses thereof
patent, September 1996

Sorge, Joseph A.; Mullinax, Rebecca Lynn
US Patent Document 5,556,772
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5556772

T7 DNA polymerase
patent, July 1990

Tabor, Stanley; Richardson, Charles C.
US Patent Document 4,942,130
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=4942130

Works referencing / citing this record:

Nucleic acid amplification
patent, July 2018

Patel, Pranav
US Patent Document 10,017,809
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10017809

Nucleic acid amplification
patent, November 2018

Belhocine, Kamila; Lee, Josephine; Patel, Pranav
US Patent Document 10,131,939
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10131939

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Similar Records

Title: Isothermal amplification of DNA

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