Isothermal amplification of DNA

Title: Isothermal amplification of DNA

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Abstract

A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.

Inventors:: Tabor, Stanley; Richardson, Charles C.

Issue Date:: 2014-04-29

Research Org.:: Harvard Univ., Cambridge, MA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1532093

Patent Number(s):: 8709724

Application Number:: 10/813,693

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q2522/101 - Single or double stranded nucleic acid binding proteins
C12Q2523/313 - Irradiation, e.g. UV irradiation
C12Q2521/501 - Ligase
C12Q2521/525 - Phosphatase
C12Q2527/101 - Temperature
C12Q1/6844 - Nucleic acid amplification reactions
C12Q2521/513 - Winding/unwinding enzyme, e.g. helicase

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DOE Contract Number:: FG02-96ER62251

Resource Type:: Patent

Resource Relation:: Patent File Date: 2003-11-07

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Tabor, Stanley, and Richardson, Charles C. Isothermal amplification of DNA.  United States: N. p., 2014. 
        Web.

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                    Tabor, Stanley, & Richardson, Charles C. Isothermal amplification of DNA.  United States.

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                    Tabor, Stanley, and Richardson, Charles C. Tue .  
        "Isothermal amplification of DNA".  United States.  https://www.osti.gov/servlets/purl/1532093.

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@article{osti_1532093,

  title        = {Isothermal amplification of DNA},

  author       = {Tabor, Stanley and Richardson, Charles C.},

  abstractNote = {A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2014},

  month        = {4}

}

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