Isothermal amplification of DNA
Abstract
A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.
- Inventors:
- Issue Date:
- Research Org.:
- Harvard Univ., Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1532093
- Patent Number(s):
- 8709724
- Application Number:
- 10/813,693
- Assignee:
- President and Fellows of Harvard College (Cambridge, MA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-96ER62251
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2003-11-07
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Tabor, Stanley, and Richardson, Charles C. Isothermal amplification of DNA. United States: N. p., 2014.
Web.
Tabor, Stanley, & Richardson, Charles C. Isothermal amplification of DNA. United States.
Tabor, Stanley, and Richardson, Charles C. Tue .
"Isothermal amplification of DNA". United States. https://www.osti.gov/servlets/purl/1532093.
@article{osti_1532093,
title = {Isothermal amplification of DNA},
author = {Tabor, Stanley and Richardson, Charles C.},
abstractNote = {A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {4}
}
Works referenced in this record:
T7 DNA polymerase
patent, August 1990
- Tabor, Stanley; Richardson, Charles C.
- US Patent Document 4,946,786
Promoter ligation activated transcription amplification of nucleic acid sequences
patent, March 1993
- Berninger, Mark S.; Schuster, David M.; Rashtchian, Ayoub
- US Patent Document 5,194,370
Polymerase compositions and uses thereof
patent, September 1996
- Sorge, Joseph A.; Mullinax, Rebecca Lynn
- US Patent Document 5,556,772
T7 DNA polymerase
patent, July 1990
- Tabor, Stanley; Richardson, Charles C.
- US Patent Document 4,942,130
Works referencing / citing this record:
Nucleic acid amplification
patent, November 2018
- Belhocine, Kamila; Lee, Josephine; Patel, Pranav
- US Patent Document 10,131,939