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Title: Isothermal amplification of DNA

Abstract

A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.

Inventors:
;
Issue Date:
Research Org.:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1532093
Patent Number(s):
8709724
Application Number:
10/813,693
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-96ER62251
Resource Type:
Patent
Resource Relation:
Patent File Date: 2003-11-07
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Tabor, Stanley, and Richardson, Charles C. Isothermal amplification of DNA. United States: N. p., 2014. Web.
Tabor, Stanley, & Richardson, Charles C. Isothermal amplification of DNA. United States.
Tabor, Stanley, and Richardson, Charles C. Tue . "Isothermal amplification of DNA". United States. https://www.osti.gov/servlets/purl/1532093.
@article{osti_1532093,
title = {Isothermal amplification of DNA},
author = {Tabor, Stanley and Richardson, Charles C.},
abstractNote = {A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {4}
}

Patent:

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