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Title: Isothermal amplification of DNA

Abstract

A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.

Inventors:
;
Issue Date:
Research Org.:
Harvard College, Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1532093
Patent Number(s):
8,709,724
Application Number:
10/813,693
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
DOE Contract Number:  
FG02-96ER62251
Resource Type:
Patent
Resource Relation:
Patent File Date: 2003-11-07
Country of Publication:
United States
Language:
English

Citation Formats

Tabor, Stanley, and Richardson, Charles C. Isothermal amplification of DNA. United States: N. p., 2014. Web.
Tabor, Stanley, & Richardson, Charles C. Isothermal amplification of DNA. United States.
Tabor, Stanley, and Richardson, Charles C. Tue . "Isothermal amplification of DNA". United States. https://www.osti.gov/servlets/purl/1532093.
@article{osti_1532093,
title = {Isothermal amplification of DNA},
author = {Tabor, Stanley and Richardson, Charles C.},
abstractNote = {A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {4}
}

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Works referenced in this record:

T7 DNA polymerase
patent, August 1990


Promoter ligation activated transcription amplification of nucleic acid sequences
patent, March 1993


Polymerase compositions and uses thereof
patent, September 1996


T7 DNA polymerase
patent, July 1990


    Works referencing / citing this record:

    Nucleic acid amplification
    patent, July 2018


    Nucleic acid amplification
    patent, November 2018