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Title: Innovative nanopore sequencing technology including a self-assembled porous membrane

Abstract

Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1532065
Patent Number(s):
9927397
Application Number:
14/555,453
Assignee:
STC.UNM (Albuquerque, NM)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC04-94AL85000; FG02-02ER15368; W911NF-08-1-0214; W911NF-11-00080
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014-11-26
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; 77 NANOSCIENCE AND NANOTECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Brueck, Steven R. J., Edwards, Jeremy Scott, Neumann, Alexander, Kuznetsova, Yuliya, Mendoza, Edgar A., and Brinker, C. Jeffrey. Innovative nanopore sequencing technology including a self-assembled porous membrane. United States: N. p., 2018. Web.
Brueck, Steven R. J., Edwards, Jeremy Scott, Neumann, Alexander, Kuznetsova, Yuliya, Mendoza, Edgar A., & Brinker, C. Jeffrey. Innovative nanopore sequencing technology including a self-assembled porous membrane. United States.
Brueck, Steven R. J., Edwards, Jeremy Scott, Neumann, Alexander, Kuznetsova, Yuliya, Mendoza, Edgar A., and Brinker, C. Jeffrey. Tue . "Innovative nanopore sequencing technology including a self-assembled porous membrane". United States. https://www.osti.gov/servlets/purl/1532065.
@article{osti_1532065,
title = {Innovative nanopore sequencing technology including a self-assembled porous membrane},
author = {Brueck, Steven R. J. and Edwards, Jeremy Scott and Neumann, Alexander and Kuznetsova, Yuliya and Mendoza, Edgar A. and Brinker, C. Jeffrey},
abstractNote = {Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {3}
}

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