Title: Innovative nanopore sequencing technology including a self-assembled porous membrane

Abstract

Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

Inventors:

Brueck, Steven R. J.; Edwards, Jeremy Scott; Neumann, Alexander; Kuznetsova, Yuliya; Mendoza, Edgar A.; Brinker, C. Jeffrey

Issue Date:

2018-03-27

Research Org.:

Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1532065

Patent Number(s):

9927397

Application Number:

14/555,453

Assignee:

STC.UNM (Albuquerque, NM)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2200/0663 - Stretching or orienting elongated molecules or particles
B01L2300/0645 - Electrodes
B01L2300/0896 - Nanoscaled
B01L2400/0421 - electrophoretic flow
B01L3/502707 - {characterised by the manufacture of the container or its components
B01L3/50273 - {characterised by the means or forces applied to move the fluids
B01L3/502746 - {characterised by the means for controlling flow resistance, e.g. flow controllers, baffles

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6869 - Methods for sequencing
C12Q2563/157 - Nanotubes or nanorods
C12Q2563/159 - Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
C12Q2565/631 - being a biochannel or pore

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N27/44791 - {Microapparatus
G01N33/48721 - {Investigating individual macromolecules, e.g. by translocation through nanopores

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DOE Contract Number:  

AC04-94AL85000; FG02-02ER15368; W911NF-08-1-0214; W911NF-11-00080

Resource Type:

Patent

Resource Relation:

Patent File Date: 2014-11-26

Country of Publication:

United States

Language:

English

Subject:

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; 77 NANOSCIENCE AND NANOTECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY