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Title: Development of sensitive FRET sensors and methods of using the same

Abstract

Intramolecular biosensors are disclosed, including PBP-based biosensors, comprising a ligand binding domain fused to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding ligand. At least one of the donor and fluorescent moieties may be internally fused to the biosensor such that both ends of the internally fused fluorophore are fixed. In addition, methods of improving the sensitivity of terminally fused biosensors are provided. The biosensors of the invention are useful for the detection and quantification of ligands in vivo and in culture.

Inventors:
; ; ;
Issue Date:
Research Org.:
Carnegie Inst. of Washington, Washington, DC (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531872
Patent Number(s):
8530633
Application Number:
11/665,339
Assignee:
Carnegie Institution of Washington (Washington, DC)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-04ER15542
Resource Type:
Patent
Resource Relation:
Patent File Date: 2005-10-14
Country of Publication:
United States
Language:
English

Citation Formats

Frommer, Wolf B., Okumoto, Sakiko, Looger, Loren, and Fehr, Marcus. Development of sensitive FRET sensors and methods of using the same. United States: N. p., 2013. Web.
Frommer, Wolf B., Okumoto, Sakiko, Looger, Loren, & Fehr, Marcus. Development of sensitive FRET sensors and methods of using the same. United States.
Frommer, Wolf B., Okumoto, Sakiko, Looger, Loren, and Fehr, Marcus. Tue . "Development of sensitive FRET sensors and methods of using the same". United States. https://www.osti.gov/servlets/purl/1531872.
@article{osti_1531872,
title = {Development of sensitive FRET sensors and methods of using the same},
author = {Frommer, Wolf B. and Okumoto, Sakiko and Looger, Loren and Fehr, Marcus},
abstractNote = {Intramolecular biosensors are disclosed, including PBP-based biosensors, comprising a ligand binding domain fused to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding ligand. At least one of the donor and fluorescent moieties may be internally fused to the biosensor such that both ends of the internally fused fluorophore are fixed. In addition, methods of improving the sensitivity of terminally fused biosensors are provided. The biosensors of the invention are useful for the detection and quantification of ligands in vivo and in culture.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {9}
}

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    Works referencing / citing this record:

    pH-insensitive glucose indicator protein
    patent, November 2017