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Title: Neurotransmitter sensors and methods of using the same

Abstract

Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.

Inventors:
; ;
Issue Date:
Research Org.:
Carnegie Inst. of Washington, Washington, DC (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531775
Patent Number(s):
8354250
Application Number:
12/823,796
Assignee:
Carnegie Institution of Washington (Washington, DC)
Patent Classifications (CPCs):
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
FG02-04ER15542
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010-06-25
Country of Publication:
United States
Language:
English

Citation Formats

Okumoto, Sakiko, Looger, Loren L., and Frommer, Wolf B. Neurotransmitter sensors and methods of using the same. United States: N. p., 2013. Web.
Okumoto, Sakiko, Looger, Loren L., & Frommer, Wolf B. Neurotransmitter sensors and methods of using the same. United States.
Okumoto, Sakiko, Looger, Loren L., and Frommer, Wolf B. Tue . "Neurotransmitter sensors and methods of using the same". United States. https://www.osti.gov/servlets/purl/1531775.
@article{osti_1531775,
title = {Neurotransmitter sensors and methods of using the same},
author = {Okumoto, Sakiko and Looger, Loren L. and Frommer, Wolf B.},
abstractNote = {Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {1}
}

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Works referenced in this record:

Reductive, energy-transfer fluorogenic probes
patent, August 1998


Fluorescent protein indicators
patent, October 2002


Engineered proteins for analyte sensing
patent, March 2001


Tandem fluorescent protein constructs
patent, November 1999