Title: Neurotransmitter sensors and methods of using the same

Abstract

Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.

Inventors:

Okumoto, Sakiko; Looger, Loren L.; Frommer, Wolf B.

Issue Date:

2013-01-15

Research Org.:

Carnegie Inst. of Washington, Washington, DC (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1531775

Patent Number(s):

8354250

Application Number:

12/823,796

Assignee:

Carnegie Institution of Washington (Washington, DC)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

A61P9/00 - Drugs for disorders of the cardiovascular system

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/9406 - {Neurotransmitters}
G01N2500/00 - Screening for compounds of potential therapeutic value
G01N33/542 - with steric inhibition or signal modification, e.g. fluorescent quenching

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

A61P25/08 - Antiepileptics
A61P25/28 - for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
A61P21/00 - Drugs for disorders of the muscular or neuromuscular system
A61P31/18 - for HIV
A61P25/14 - for treating abnormal movements, e.g. chorea, dyskinesia

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DOE Contract Number:  

FG02-04ER15542

Resource Type:

Patent

Resource Relation:

Patent File Date: 2010-06-25

Country of Publication:

United States

Language:

English