Neurotransmitter sensors and methods of using the same

Title: Neurotransmitter sensors and methods of using the same

Abstract

Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.

Inventors:: Okumoto, Sakiko; Looger, Loren L.; Frommer, Wolf B.

Issue Date:: 2013-01-15

Research Org.:: Carnegie Inst. of Washington, Washington, DC (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1531775

Patent Number(s):: 8,354,250

Application Number:: 12/823,796

Assignee:: Carnegie Institution of Washington (Washington, DC)

DOE Contract Number:: FG02-04ER15542

Resource Type:: Patent

Resource Relation:: Patent File Date: 2010-06-25

Country of Publication:: United States

Language:: English

Citation Formats


                    Okumoto, Sakiko, Looger, Loren L., and Frommer, Wolf B. Neurotransmitter sensors and methods of using the same.  United States: N. p., 2013. 
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                    Okumoto, Sakiko, Looger, Loren L., & Frommer, Wolf B. Neurotransmitter sensors and methods of using the same.  United States.

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                    Okumoto, Sakiko, Looger, Loren L., and Frommer, Wolf B. Tue .  
        "Neurotransmitter sensors and methods of using the same".  United States.  https://www.osti.gov/servlets/purl/1531775.

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@article{osti_1531775,

  title        = {Neurotransmitter sensors and methods of using the same},

  author       = {Okumoto, Sakiko and Looger, Loren L. and Frommer, Wolf B.},

  abstractNote = {Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2013},

  month        = {1}

}

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Works referenced in this record:

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Lakowicz, Joseph R.; Tolosa, Leah; Eichhorn, Lisa
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Title: Neurotransmitter sensors and methods of using the same

Abstract

Citation Formats

Reductive, energy-transfer fluorogenic probes patent, August 1998

Fluorescent protein indicators patent, October 2002

Engineered proteins for analyte sensing patent, March 2001

Detection by fret changes of ligand binding by GFP fusion proteins patent, April 2002

Identification of essential genes in microorganisms patent-application, February 2004

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patent, March 2001

Detection by fret changes of ligand binding by GFP fusion proteins
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