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Title: Methods of obtaining active antisense compounds

Abstract

Methods for obtaining antisense oligonucleotides with activity against a desired target are provided. Methods of identifying oligonucleotide sequence motifs which are predictive of antisense oligonucleotide activity are provided, as are motifs identified according to this method. Methods of selecting effective antisense oligonucleotide sequences and effective antisense target sequences are provided, as are sequences selected according to these methods. In other methods of the invention, oligonucleotides are designed to hybridize to target sequences containing one or more activity-enhancing motifs. Antisense oligonucleotides designed according to these methods are also provided.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Isis Phramaceuticals, Inc., Carlsbad, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531738
Patent Number(s):
8202982
Application Number:
12/557,358
Assignee:
Isis Pharmaceuticals, Inc. (Carlsbad, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
DOE Contract Number:  
FG03-99ER62732
Resource Type:
Patent
Resource Relation:
Patent File Date: 2009-09-10
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Freier, Susan M., Matveeva, Olga, Tsodikov, Alexander, Giddings, Michael C., and Wyatt, Jacqueline R. Methods of obtaining active antisense compounds. United States: N. p., 2012. Web.
Freier, Susan M., Matveeva, Olga, Tsodikov, Alexander, Giddings, Michael C., & Wyatt, Jacqueline R. Methods of obtaining active antisense compounds. United States.
Freier, Susan M., Matveeva, Olga, Tsodikov, Alexander, Giddings, Michael C., and Wyatt, Jacqueline R. Tue . "Methods of obtaining active antisense compounds". United States. https://www.osti.gov/servlets/purl/1531738.
@article{osti_1531738,
title = {Methods of obtaining active antisense compounds},
author = {Freier, Susan M. and Matveeva, Olga and Tsodikov, Alexander and Giddings, Michael C. and Wyatt, Jacqueline R.},
abstractNote = {Methods for obtaining antisense oligonucleotides with activity against a desired target are provided. Methods of identifying oligonucleotide sequence motifs which are predictive of antisense oligonucleotide activity are provided, as are motifs identified according to this method. Methods of selecting effective antisense oligonucleotide sequences and effective antisense target sequences are provided, as are sequences selected according to these methods. In other methods of the invention, oligonucleotides are designed to hybridize to target sequences containing one or more activity-enhancing motifs. Antisense oligonucleotides designed according to these methods are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {6}
}

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Works referenced in this record:

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