Title: Sucrose biosensors and methods of using the same

Abstract

Sucrose biosensors are disclosed, which comprise a sucrose binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon sucrose binding. Such biosensors are useful for real time monitoring of sucrose metabolism in living cells.

Inventors:

Frommer, Wolf B.; Lager, Ida

Issue Date:

Tue May 08 00:00:00 EDT 2012

Research Org.:

Carnegie Institution of Washington, Washington, D.C. (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1531733

Patent Number(s):

8173863

Application Number:

12/083,196

Assignee:

Carnegie Institution of Washington (Washington, DC)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/195 - from bacteria
C07K2319/20 - containing a tag with affinity for a non-protein ligand
C07K2319/60 - containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/025 - {for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/5044 - {involving specific cell types}

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DOE Contract Number:  

FG02-04ER15542

Resource Type:

Patent

Resource Relation:

Patent File Date: 2005-10-14

Country of Publication:

United States

Language:

English