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Title: Methods for the synthesis of arrays of DNA probes

Abstract

The synthesis of arrays of DNA probes sequences, polypeptides, and the like is carried out using a patterning process on an active surface of a substrate. An image is projected onto the active surface of the substrate utilizing reflective projection optics. The projection optics project a light image onto the active surface of the substrate to deprotect linker molecules thereon. A first level of bases may then be applied to the substrate, followed by development steps, and subsequent exposure of the substrate utilizing a different light image, with further repeats until the elements of a two dimensional array on the substrate surface have an appropriate base bound thereto.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Wisconsin Alumni Research Foundation, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531700
Patent Number(s):
8030477
Application Number:
11/524,082
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
DOE Contract Number:  
FG02-88ER13938; FG02-96ER45569; P071760302; P0062242-02; 63040304
Resource Type:
Patent
Resource Relation:
Patent File Date: 2006-09-20
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Cerrina, Francesco, Sussman, Michael R., Blattner, Frederick R., Singh-Gasson, Sangeet, and Green, Roland. Methods for the synthesis of arrays of DNA probes. United States: N. p., 2011. Web.
Cerrina, Francesco, Sussman, Michael R., Blattner, Frederick R., Singh-Gasson, Sangeet, & Green, Roland. Methods for the synthesis of arrays of DNA probes. United States.
Cerrina, Francesco, Sussman, Michael R., Blattner, Frederick R., Singh-Gasson, Sangeet, and Green, Roland. Tue . "Methods for the synthesis of arrays of DNA probes". United States. https://www.osti.gov/servlets/purl/1531700.
@article{osti_1531700,
title = {Methods for the synthesis of arrays of DNA probes},
author = {Cerrina, Francesco and Sussman, Michael R. and Blattner, Frederick R. and Singh-Gasson, Sangeet and Green, Roland},
abstractNote = {The synthesis of arrays of DNA probes sequences, polypeptides, and the like is carried out using a patterning process on an active surface of a substrate. An image is projected onto the active surface of the substrate utilizing reflective projection optics. The projection optics project a light image onto the active surface of the substrate to deprotect linker molecules thereon. A first level of bases may then be applied to the substrate, followed by development steps, and subsequent exposure of the substrate utilizing a different light image, with further repeats until the elements of a two dimensional array on the substrate surface have an appropriate base bound thereto.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {10}
}

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