Title: Methods for the synthesis of arrays of DNA probes

Abstract

The synthesis of arrays of DNA probes sequences, polypeptides, and the like is carried out using a patterning process on an active surface of a substrate. An image is projected onto the active surface of the substrate utilizing reflective projection optics. The projection optics project a light image onto the active surface of the substrate to deprotect linker molecules thereon. A first level of bases may then be applied to the substrate, followed by development steps, and subsequent exposure of the substrate utilizing a different light image, with further repeats until the elements of a two dimensional array on the substrate surface have an appropriate base bound thereto.

Inventors:

Cerrina, Francesco; Sussman, Michael R.; Blattner, Frederick R.; Singh-Gasson, Sangeet; Green, Roland

Issue Date:

Tue Oct 04 00:00:00 EDT 2011

Research Org.:

Wisconsin Alumni Research Foundation, Madison, WI (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1531700

Patent Number(s):

8030477

Application Number:

11/524,082

Assignee:

Wisconsin Alumni Research Foundation (Madison, WI)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J19/0046 - {Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides
B01J2219/00439 - using micromirror arrays
B01J2219/00527 - Sheets
B01J2219/00529 - DNA chips
B01J2219/00585 - Parallel processes
B01J2219/0059 - Sequential processes
B01J2219/00596 - Solid-phase processes
B01J2219/00605 - the compounds being directly bound or immobilised to solid supports
B01J2219/00608 - DNA chips
B01J2219/00612 - the surface being inorganic
B01J2219/00626 - Covalent
B01J2219/00637 - by coating it with another layer
B01J2219/00641 - the porous medium being continuous, e.g. porous oxide substrates
B01J2219/00659 - Two-dimensional arrays
B01J2219/00689 - using computers
B01J2219/00711 - Light-directed synthesis
B01J2219/00722 - Nucleotides

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

B82Y30/00 - Nanotechnology for materials or surface science, e.g. nanocomposites

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07B - GENERAL METHODS OF ORGANIC CHEMISTRY

C07B2200/11 - Compounds covalently bound to a solid support

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS

C07H21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

C - CHEMISTRY C40 - COMBINATORIAL TECHNOLOGY C40B - COMBINATORIAL CHEMISTRY

C40B40/00 - Libraries per se, e.g. arrays, mixtures
C40B40/06 - Libraries containing nucleotides or polynucleotides, or derivatives thereof
C40B60/14 - for creating libraries

G - PHYSICS G02 - OPTICS G02B - OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS

G02B26/0841 - {the reflecting element being moved or deformed by electrostatic means}

G - PHYSICS G03 - PHOTOGRAPHY G03F - PHOTOMECHANICAL PRODUCTION OF TEXTURED OR PATTERNED SURFACES, e.g. FOR PRINTING, FOR PROCESSING OF SEMICONDUCTOR DEVICES

G03F7/2002 - {with visible light or UV light, through an original having an opaque pattern on a transparent support, e.g. film printing, projection printing

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DOE Contract Number:  

FG02-88ER13938; FG02-96ER45569; P071760302; P0062242-02; 63040304

Resource Type:

Patent

Resource Relation:

Patent File Date: 2006-09-20

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES