Neurotransmitter sensors and methods of using the same

Okumoto, Sakiko; Looger, Loren L.; Frommer, Wolf B.

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Title: Neurotransmitter sensors and methods of using the same

Abstract

Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.

Inventors:: Okumoto, Sakiko; Looger, Loren L.; Frommer, Wolf B.

Issue Date:: Tue Aug 17 00:00:00 EDT 2010

Research Org.:: Carnegie Inst. of Washington, Washington, DC (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1531646

Patent Number(s):: 7777016

Application Number:: 11/665,343

Assignee:: Carnegie Institution of Washington (Washington, DC)

Patent Classifications (CPCs):: A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

Show more

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
A61P21/00 - Drugs for disorders of the muscular or neuromuscular system
A61P25/08 - Antiepileptics
A61P25/14 - for treating abnormal movements, e.g. chorea, dyskinesia
A61P25/28 - for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
A61P31/18 - for HIV
A61P9/00 - Drugs for disorders of the cardiovascular system

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2500/00 - Screening for compounds of potential therapeutic value
G01N33/542 - with steric inhibition or signal modification, e.g. fluorescent quenching
G01N33/9406 - {Neurotransmitters}

Show less

DOE Contract Number:: FG02-04ER15542

Resource Type:: Patent

Resource Relation:: Patent File Date: 2005-10-14

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

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                    Okumoto, Sakiko, Looger, Loren L., and Frommer, Wolf B. Neurotransmitter sensors and methods of using the same.  United States: N. p., 2010. 
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                    Okumoto, Sakiko, Looger, Loren L., & Frommer, Wolf B. Neurotransmitter sensors and methods of using the same.  United States.

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                    Okumoto, Sakiko, Looger, Loren L., and Frommer, Wolf B. Tue .  
        "Neurotransmitter sensors and methods of using the same".  United States.  https://www.osti.gov/servlets/purl/1531646.

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@article{osti_1531646,

  title        = {Neurotransmitter sensors and methods of using the same},

  author       = {Okumoto, Sakiko and Looger, Loren L. and Frommer, Wolf B.},

  abstractNote = {Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Aug 17 00:00:00 EDT 2010},

  month        = {Tue Aug 17 00:00:00 EDT 2010}

}

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Similar Records

Title: Neurotransmitter sensors and methods of using the same

Abstract

Citation Formats

Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein journal, February 1999

Engineered proteins for analyte sensing patent, March 2001

Compositions and methods for measuring analyte concentrations patent-application, May 2005

Protein localization in living cells and tissues using FRET and FLIM journal, December 2003

A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications journal, January 2002

Visualization of maltose uptake in living yeast cells by fluorescent nanosensors journal, July 2002

Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin journal, August 1997

Optimized Heterologous Expression of the Polymorphic Human Glutathione Transferase M1-1 Based on Silent Mutations in the Corresponding cDNA journal, June 1996

Reductive, energy-transfer fluorogenic probes patent, August 1998

Design of Bioelectronic Interfaces by Exploiting Hinge-Bending Motions in Proteins journal, August 2001

The Genome of the Natural Genetic Engineer Agrobacterium tumefaciens C58 journal, December 2001

Fluorescent protein sensors for detection of analytes patent, December 1999

Method and system for rapid biomolecular recognition of amino acids and protein sequencing patent-application, May 2002

X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins journal, January 2004

Novel biosensors for the detection of estrogen receptor ligands journal, August 2005

Fusion estrogen receptor proteins: toward the development of receptor-based agonists and antagonists journal, September 2001

Kinetic and Thermodynamic Studies of Purine Repressor Binding to Corepressor and Operator DNA journal, April 1998

Shedding Light on Cell Signaling: Interpretation of FRET Biosensors journal, January 2003

Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering journal, September 2005

Fluorescent protein indicators patent, October 2002

Fluorescence Quenching Studies of Trp Repressor–Operator Interaction journal, November 1999

Tandem fluorescent protein constructs patent, November 1999

Construction of a fluorescent biosensor family journal, November 2002

Compositions and methods for monitoring the modification of natural binding partners patent, October 2002

Monomeric and dimeric fluorescent protein variants and methods for making same patent-application, September 2005

Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor. journal, December 1980

Detection by fret changes of ligand binding by GFP fusion proteins patent, April 2002

Identification of essential genes in microorganisms patent-application, February 2004

Building and breeding molecules to spy on cells and tumors journal, November 2004

Strategies for optimizing heterologous protein expression in Escherichia coli journal, February 1998

Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering journal, December 2001

Biosensor patent-application, June 2004

Domain dislocation: a change of core structure in periplasmic binding proteins in their evolutionary history journal, February 1999

Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors journal, June 2005

Biosensor patent, August 2001

Binding protein as biosensors patent-application, July 2003

Periplasmic binding proteins: a versatile superfamily for protein engineering journal, August 2004

Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein journal, January 1996

Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein
journal, February 1999

Engineered proteins for analyte sensing
patent, March 2001

Compositions and methods for measuring analyte concentrations
patent-application, May 2005

Protein localization in living cells and tissues using FRET and FLIM
journal, December 2003

A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
journal, January 2002

Visualization of maltose uptake in living yeast cells by fluorescent nanosensors
journal, July 2002

Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
journal, August 1997

Optimized Heterologous Expression of the Polymorphic Human Glutathione Transferase M1-1 Based on Silent Mutations in the Corresponding cDNA
journal, June 1996

Reductive, energy-transfer fluorogenic probes
patent, August 1998

Design of Bioelectronic Interfaces by Exploiting Hinge-Bending Motions in Proteins
journal, August 2001

The Genome of the Natural Genetic Engineer Agrobacterium tumefaciens C58
journal, December 2001

Fluorescent protein sensors for detection of analytes
patent, December 1999

Method and system for rapid biomolecular recognition of amino acids and protein sequencing
patent-application, May 2002

X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins
journal, January 2004

Novel biosensors for the detection of estrogen receptor ligands
journal, August 2005

Fusion estrogen receptor proteins: toward the development of receptor-based agonists and antagonists
journal, September 2001

Kinetic and Thermodynamic Studies of Purine Repressor Binding to Corepressor and Operator DNA
journal, April 1998

Shedding Light on Cell Signaling: Interpretation of FRET Biosensors
journal, January 2003

Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering
journal, September 2005

Fluorescent protein indicators
patent, October 2002

Fluorescence Quenching Studies of Trp Repressor–Operator Interaction
journal, November 1999

Tandem fluorescent protein constructs
patent, November 1999

Construction of a fluorescent biosensor family
journal, November 2002

Compositions and methods for monitoring the modification of natural binding partners
patent, October 2002

Monomeric and dimeric fluorescent protein variants and methods for making same
patent-application, September 2005

Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor.
journal, December 1980

Detection by fret changes of ligand binding by GFP fusion proteins
patent, April 2002

Identification of essential genes in microorganisms
patent-application, February 2004

Building and breeding molecules to spy on cells and tumors
journal, November 2004

Strategies for optimizing heterologous protein expression in Escherichia coli
journal, February 1998

Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering
journal, December 2001

Biosensor
patent-application, June 2004

Domain dislocation: a change of core structure in periplasmic binding proteins in their evolutionary history
journal, February 1999

Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors
journal, June 2005

Biosensor
patent, August 2001

Binding protein as biosensors
patent-application, July 2003

Periplasmic binding proteins: a versatile superfamily for protein engineering
journal, August 2004

Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein
journal, January 1996