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Title: Neurotransmitter sensors and methods of using the same

Abstract

Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.

Inventors:
; ;
Issue Date:
Research Org.:
Carnegie Inst. of Washington, Washington, DC (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531646
Patent Number(s):
7777016
Application Number:
11/665,343
Assignee:
Carnegie Institution of Washington (Washington, DC)
Patent Classifications (CPCs):
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
FG02-04ER15542
Resource Type:
Patent
Resource Relation:
Patent File Date: 2005-10-14
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Okumoto, Sakiko, Looger, Loren L., and Frommer, Wolf B. Neurotransmitter sensors and methods of using the same. United States: N. p., 2010. Web.
Okumoto, Sakiko, Looger, Loren L., & Frommer, Wolf B. Neurotransmitter sensors and methods of using the same. United States.
Okumoto, Sakiko, Looger, Loren L., and Frommer, Wolf B. Tue . "Neurotransmitter sensors and methods of using the same". United States. https://www.osti.gov/servlets/purl/1531646.
@article{osti_1531646,
title = {Neurotransmitter sensors and methods of using the same},
author = {Okumoto, Sakiko and Looger, Loren L. and Frommer, Wolf B.},
abstractNote = {Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {8}
}

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