Micro-channel long molecule manipulation system

Schwartz, David Charles; Dimalanta, Eileen T.; de Pablo, Juan J.

Title: Micro-channel long molecule manipulation system

Abstract

Laminar flow of a carrier liquid and polymeric molecules through micro-channels is used to straighten, align, separate, and/or sort the polymeric molecules. The polymeric molecules may be analyzed and/or manipulated in the carrier liquid or attached to a wall of the micro-channel for subsequent treatment and analysis. Micro-channels can be manufactured using an elastic molding material. One micro-channel embodiment provides fluid flow using a standard laboratory centrifuge.

Inventors:: Schwartz, David Charles; Dimalanta, Eileen T.; de Pablo, Juan J.

Issue Date:: 2010-08-17

Research Org.:: Wisconsin Alumni Research Foundation, Madison, WI (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1531644

Patent Number(s):: 7775368

Application Number:: 10/688,416

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Classifications (CPCs):: B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2200/0663 - Stretching or orienting elongated molecules or particles
B01L2300/0816 - Cards, e.g. flat sample carriers usually with flow in two horizontal directions
B01L2400/0409 - centrifugal forces
B01L2400/0415 - electrical forces, e.g. electrokinetic
B01L2400/0478 - pistons
B01L2400/0481 - squeezing of channels or chambers
B01L3/502761 - {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N15/0255 - {with mechanical, e.g. inertial, classification, and investigation of sorted collections
G01N2015/0288 - {Sorting the particles}

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DOE Contract Number:: FG02-99ER62830; NIH HG00225

Resource Type:: Patent

Resource Relation:: Patent File Date: 2003-10-17

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

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                    Schwartz, David Charles, Dimalanta, Eileen T., and de Pablo, Juan J. Micro-channel long molecule manipulation system.  United States: N. p., 2010. 
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                    Schwartz, David Charles, Dimalanta, Eileen T., & de Pablo, Juan J. Micro-channel long molecule manipulation system.  United States.

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                    Schwartz, David Charles, Dimalanta, Eileen T., and de Pablo, Juan J. Tue .  
        "Micro-channel long molecule manipulation system".  United States.  https://www.osti.gov/servlets/purl/1531644.

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@article{osti_1531644,

  title        = {Micro-channel long molecule manipulation system},

  author       = {Schwartz, David Charles and Dimalanta, Eileen T. and de Pablo, Juan J.},

  abstractNote = {Laminar flow of a carrier liquid and polymeric molecules through micro-channels is used to straighten, align, separate, and/or sort the polymeric molecules. The polymeric molecules may be analyzed and/or manipulated in the carrier liquid or attached to a wall of the micro-channel for subsequent treatment and analysis. Micro-channels can be manufactured using an elastic molding material. One micro-channel embodiment provides fluid flow using a standard laboratory centrifuge.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2010},

  month        = {8}

}

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Laminar flow of a carrier liquid and polymeric molecules through micro-channels is used to straighten the polymeric molecules and attach the straightened molecules to a wall of the micro-channel for subsequent treatment and analysis. Micro-channels can be manufactured using an elastic molding material. One micro-channel embodiment provides fluid flow using a standard laboratory centrifuge.
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Micro injector sample delivery system for charged molecules

Patent Davidson, James C [Livermore, CA]; Balch, Joseph W [Livermore, CA]

A micro injector sample delivery system for charged molecules. The injector is used for collecting and delivering controlled amounts of charged molecule samples for subsequent analysis. The injector delivery system can be scaled to large numbers (>96) for sample delivery to massively parallel high throughput analysis systems. The essence of the injector system is an electric field controllable loading tip including a section of porous material. By applying the appropriate polarity bias potential to the injector tip, charged molecules will migrate into porous material, and by reversing the polarity bias potential the molecules are ejected or forced away from themore » « less
Full Text Available
Method And Apparatus For Reducing Sample Dispersion In Turns And Junctions Of Micro-Channel Systems

Patent Griffiths, Stewart K. (Danville, CA), Nilson, Robert H. (Cardiff-by-the-Sea, CA)

What is disclosed pertains to improvement in the performance of microchannel devices by providing turns, wyes, tees, and other junctions that produce little dispersion of a sample as it traverses the turn or junction. The reduced dispersion results from contraction and expansion regions that reduce the cross-sectional area over some portion of the turn or junction. By carefully designing the geometries of these regions, sample dispersion in turns and junctions is reduced to levels comparable to the effects of ordinary diffusion. The low dispersion features are particularly suited for microfluidic devices and systems using either electromotive force, pressure, or combinationsmore » « less
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Micro-channel cooling of integrated combustor nozzle of a segmented annular combustion system

Patent Berry, Jonathan Dwight; Hughes, Michael John

A segmented annular combustion system includes integrated combustor nozzles, each of which has a fuel injection panel disposed radially between an inner liner segment and an outer liner segment. The fuel injection panel includes an aft end portion, a first side wall, a second side wall, premixing channels defined between the first side wall and the side wall, and injection outlets defined along at least one of the first side wall and the second side wall. The aft end portion defines a turbine nozzle portion. An interior portion between the first side wall and the second side wall includes wallsmore » « less
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Dual low vacuum-ultrahigh vacuum system for large-scale production of micro-channel plate photomultipliers

Patent Frisch, Henry J.; Angelico, Evan; Elagin, Andrey; ...

Systems and methods for the batch production of large numbers of highly uniform multichannel-plate photomultiplier tubes (MCP-PMTs) for large-scale applications are provided. The systems and methods employ dual, nested low-vacuum (LV) and UHV processing in a rapid-cycling, small-footprint, scalable, batch-production facility that is capable of fabricating many MCP-PMTs simultaneously.
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Title: Micro-channel long molecule manipulation system

Abstract

Citation Formats

Flow properties of high-molecular-weight DNA solutions: Viscosity, recoil, and longest retardation time journal, May 1973

Application of a high-resolution TV-microscope system to estimate the sequence of centromere separation in muntjak chromosomes journal, November 1984

Alignment and sensitive detection of DNA by a moving interface journal, September 1994

[3] Immobilization of enzymes to agar, agarose, and sephadex support book, January 1976

Methods and compositions for the manipulation and characterization of individual nucleic acid molecules patent, November 2000

Cleaving yeast and Escherichia coli genomes at a single site journal, October 1990

Method and characterizing polymer molecules or the like patent, November 2000

Continuum of overlapping clones spanning the entire human chromosome 21q journal, October 1992

Electron induced fluorescent method for nucleic acid sequencing patent-application, September 2003

Techniques for Chromosome Analysis book, January 1982

Fluidics-the link between micro and nano sciences and technologies conference, January 2001

Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system patent-application, September 2002

Field-inversion gel electrophoresis patent, April 1988

ED: pulsed electrophoresis instrument journal, November 1989

Electrophoretic separations of large DNA molecules by periodic inversion of the electric field journal, April 1986

Sizing of Large DNA Molecules by Hook Formation in a Loose Matrix journal, August 1993

Sensitive, high-resolution chromatin and chromosome mapping in situ: Presence and orientation of two closely integrated copies of EBV in a lymphoma line journal, January 1988

High-resolution mapping of mammalian genes by in situ hybridization to free chromatin. journal, October 1992

Direct observation of tube-like motion of a single polymer chain journal, May 1994

Method for optically manipulating polymer filaments patent, January 1992

Construction of artificial chromosomes in yeast journal, September 1983

Selective cleavage of human DNA: RecA-assisted restriction endonuclease (RARE) cleavage journal, December 1991

Direct observation of DNA chain orientation and relaxation by electric birefringence: Implications for the mechanism of separation during pulsed-field gel electrophoresis journal, March 1989

Methods and apparatuses for characterization of single polymers patent-application, June 2002

Real-time imaging of single DNA molecules with fluorescence microscopy journal, September 1989

Dynamic Behaviors of DNA Molecules in Solution Studied by Fluorescence Microscopy journal, January 1983

Forces between mica surfaces bearing adsorbed polyelectrolyte, poly-L-lysine, in aqueous media journal, January 1984

Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis journal, May 1984

Thinking Big journal, June 2002

DNA condensation with polyamines journal, May 1978

A simple method for DNA restriction site mapping journal, September 1976

Integrated active flux microfluidic devices and methods patent-application, March 2002

Effect of pulsed and reversing electric fields on the orientation of linear and supercoiled DNA molecules in agarose gels journal, August 1988

Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads journal, November 1992

Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography journal, April 2000

Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model journal, September 1992

Electrophoresis using alternating transverse electric fields patent, September 1984

Image processing and analysis of individual nucleic acid molecules patent, August 2003

Process for aligning macromolecules by passage of a meniscus and applications patent, July 2001

Sizing single DNA molecules journal, October 1992

Method for imaging informational biological molecules on a semiconductor substrate patent, May 1994

Nanoelectrode arrays patent, September 2000

Mapping of mitochondrial 4S RNA genes in Xenopus laevis by electron microscopy journal, May 1978

Apparatus and method of analyzing nucleic acid molecules patent, September 1989

Polynucleotide reagents containing selectable cleavage sites patent, January 1995

A physical genome map of Pseudomonas aeruginosa PAO. journal, December 1989

Detection of particular nucleotide sequences patent, August 1988

Imaging of kinked configurations of DNA molecules undergoing orthogonal field alternating gel electrophoresis by fluorescence microscopy journal, April 1990

Genomic sequencing. journal, April 1984

A method for DNA sequencing by hybridization with oligonucleotide matrix journal, January 1991

Detection of specific sequences among DNA fragments separated by gel electrophoresis journal, November 1975

Atomic Force Microscopy Imaging of Double Stranded DNA and RNA journal, December 1992

Orientation of DNA Molecules in Agarose Gels By Pulsed Electric Fields journal, October 1985

Complete coverage of the Schizosaccharomyces pombe genome in yeast artificial chromosomes journal, July 1992

Light microscopic structure of DNA in solution studied by the 4′,6-diamidino-2-phenylindole staining method journal, October 1981

A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels. journal, May 1990

Methods and apparatuses for characterization of single polymers patent, July 2004

Method for characterizing polymer molecules or the like patent, February 1997

Image processing and analysis of individual nucleic acid molecules patent, February 1998

Atomic force microscopy of DNA in aqueous solutions journal, January 1993

Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors journal, May 1987

Physical mapping of human chromosomes by repetitive sequence fingerprinting. journal, August 1990

Image processing and analysis of individual nucleic acid molecules patent, September 2001

Mapping genomic organization by field inversion and two-dimensional gel electrophoresis: application to the murine T-cell receptor γ gene family journal, January 1988

Methods and compositions for the manipulation and characterization of individual nucleic acid molecules patent, May 2006

Gel inserts useful in electrophoresis patent, September 1987

Method for separating nucleic acids and nucleic acid probes patent, October 1991

Unitary microcapiliary and waveguide structure and method of fabrication patent, August 2002

Genetics by gel electrophoresis: the impact of pulsed field gel electrophoresis on mammalian genetics journal, January 1987

Image processing and analysis of individual nucleic acid molecules patent, January 2003

A sensitive solid phase microradioimmunoassay for anti-double stranded DNA antibodies journal, March 1981

Pulsed oriented electrophoresis patent, April 1995

Advanced microfluidics patent-application, May 2005

RecA-AC: single-site cleavage of plasmids and chromosomes at any predetermined restriction site journal, January 1992

Non-isotopic in-situ hybridization method for detection of nucleic acids patent, November 1999

Observation of individual DNA molecules undergoing gel electrophoresis journal, January 1989

Generation of a nested series of interstitial deletions in yeast artificial chromosomes carrying human DNA. journal, July 1991

Flow properties of high-molecular-weight DNA solutions: Viscosity, recoil, and longest retardation time
journal, May 1973

Application of a high-resolution TV-microscope system to estimate the sequence of centromere separation in muntjak chromosomes
journal, November 1984

Alignment and sensitive detection of DNA by a moving interface
journal, September 1994

[3] Immobilization of enzymes to agar, agarose, and sephadex support
book, January 1976

Methods and compositions for the manipulation and characterization of individual nucleic acid molecules
patent, November 2000

Cleaving yeast and Escherichia coli genomes at a single site
journal, October 1990

Method and characterizing polymer molecules or the like
patent, November 2000

Continuum of overlapping clones spanning the entire human chromosome 21q
journal, October 1992

Electron induced fluorescent method for nucleic acid sequencing
patent-application, September 2003

Techniques for Chromosome Analysis
book, January 1982

Fluidics-the link between micro and nano sciences and technologies
conference, January 2001

Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system
patent-application, September 2002

Field-inversion gel electrophoresis
patent, April 1988

ED: pulsed electrophoresis instrument
journal, November 1989

Electrophoretic separations of large DNA molecules by periodic inversion of the electric field
journal, April 1986

Sizing of Large DNA Molecules by Hook Formation in a Loose Matrix
journal, August 1993

Sensitive, high-resolution chromatin and chromosome mapping in situ: Presence and orientation of two closely integrated copies of EBV in a lymphoma line
journal, January 1988

High-resolution mapping of mammalian genes by in situ hybridization to free chromatin.
journal, October 1992

Direct observation of tube-like motion of a single polymer chain
journal, May 1994

Method for optically manipulating polymer filaments
patent, January 1992

Construction of artificial chromosomes in yeast
journal, September 1983

Selective cleavage of human DNA: RecA-assisted restriction endonuclease (RARE) cleavage
journal, December 1991

Direct observation of DNA chain orientation and relaxation by electric birefringence: Implications for the mechanism of separation during pulsed-field gel electrophoresis
journal, March 1989

Methods and apparatuses for characterization of single polymers
patent-application, June 2002

Real-time imaging of single DNA molecules with fluorescence microscopy
journal, September 1989

Dynamic Behaviors of DNA Molecules in Solution Studied by Fluorescence Microscopy
journal, January 1983

Forces between mica surfaces bearing adsorbed polyelectrolyte, poly-L-lysine, in aqueous media
journal, January 1984

Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis
journal, May 1984

Thinking Big
journal, June 2002

DNA condensation with polyamines
journal, May 1978

A simple method for DNA restriction site mapping
journal, September 1976

Integrated active flux microfluidic devices and methods
patent-application, March 2002

Effect of pulsed and reversing electric fields on the orientation of linear and supercoiled DNA molecules in agarose gels
journal, August 1988

Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads
journal, November 1992

Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography
journal, April 2000

Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model
journal, September 1992

Electrophoresis using alternating transverse electric fields
patent, September 1984

Image processing and analysis of individual nucleic acid molecules
patent, August 2003

Process for aligning macromolecules by passage of a meniscus and applications
patent, July 2001

Sizing single DNA molecules
journal, October 1992

Method for imaging informational biological molecules on a semiconductor substrate
patent, May 1994

Nanoelectrode arrays
patent, September 2000

Mapping of mitochondrial 4S RNA genes in Xenopus laevis by electron microscopy
journal, May 1978

Apparatus and method of analyzing nucleic acid molecules
patent, September 1989

Polynucleotide reagents containing selectable cleavage sites
patent, January 1995

A physical genome map of Pseudomonas aeruginosa PAO.
journal, December 1989

Detection of particular nucleotide sequences
patent, August 1988

Imaging of kinked configurations of DNA molecules undergoing orthogonal field alternating gel electrophoresis by fluorescence microscopy
journal, April 1990

Genomic sequencing.
journal, April 1984

A method for DNA sequencing by hybridization with oligonucleotide matrix
journal, January 1991

Detection of specific sequences among DNA fragments separated by gel electrophoresis
journal, November 1975

Atomic Force Microscopy Imaging of Double Stranded DNA and RNA
journal, December 1992

Orientation of DNA Molecules in Agarose Gels By Pulsed Electric Fields
journal, October 1985

Complete coverage of the Schizosaccharomyces pombe genome in yeast artificial chromosomes
journal, July 1992

Light microscopic structure of DNA in solution studied by the 4′,6-diamidino-2-phenylindole staining method
journal, October 1981

A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels.
journal, May 1990

Methods and apparatuses for characterization of single polymers
patent, July 2004

Method for characterizing polymer molecules or the like
patent, February 1997

Image processing and analysis of individual nucleic acid molecules
patent, February 1998

Atomic force microscopy of DNA in aqueous solutions
journal, January 1993

Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors
journal, May 1987

Physical mapping of human chromosomes by repetitive sequence fingerprinting.
journal, August 1990

Image processing and analysis of individual nucleic acid molecules
patent, September 2001

Mapping genomic organization by field inversion and two-dimensional gel electrophoresis: application to the murine T-cell receptor γ gene family
journal, January 1988

Methods and compositions for the manipulation and characterization of individual nucleic acid molecules
patent, May 2006

Gel inserts useful in electrophoresis
patent, September 1987

Method for separating nucleic acids and nucleic acid probes
patent, October 1991

Unitary microcapiliary and waveguide structure and method of fabrication
patent, August 2002

Genetics by gel electrophoresis: the impact of pulsed field gel electrophoresis on mammalian genetics
journal, January 1987

Image processing and analysis of individual nucleic acid molecules
patent, January 2003

A sensitive solid phase microradioimmunoassay for anti-double stranded DNA antibodies
journal, March 1981

Pulsed oriented electrophoresis
patent, April 1995

Advanced microfluidics
patent-application, May 2005

RecA-AC: single-site cleavage of plasmids and chromosomes at any predetermined restriction site
journal, January 1992

Non-isotopic in-situ hybridization method for detection of nucleic acids
patent, November 1999

Observation of individual DNA molecules undergoing gel electrophoresis
journal, January 1989

Generation of a nested series of interstitial deletions in yeast artificial chromosomes carrying human DNA.
journal, July 1991

Covalent binding of biological samples to solid supports for scanning probe microscopy in buffer solution
journal, December 1993

Conformational correlatives of DNA band compression and bidirectional migration during field inversion gel electrophoresis, detected by quantitative video epifluorescence microscopy
journal, October 1991

High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones
journal, January 1990