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Title: Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Abstract

This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.

Inventors:
; ;
Issue Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531637
Patent Number(s):
7741071
Application Number:
10/580,987
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
FG03-00ER45812; NIH GM66494
Resource Type:
Patent
Resource Relation:
Patent File Date: 2004-12-16
Country of Publication:
United States
Language:
English

Citation Formats

Zhang, Zhiwen, Alfonta, Lital, and Schultz, Peter. Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells. United States: N. p., 2010. Web.
Zhang, Zhiwen, Alfonta, Lital, & Schultz, Peter. Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells. United States.
Zhang, Zhiwen, Alfonta, Lital, and Schultz, Peter. Tue . "Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells". United States. https://www.osti.gov/servlets/purl/1531637.
@article{osti_1531637,
title = {Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells},
author = {Zhang, Zhiwen and Alfonta, Lital and Schultz, Peter},
abstractNote = {This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {6}
}

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Works referenced in this record:

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    Works referencing / citing this record:

    Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
    patent, May 2012


    Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
    patent, October 2015


    Expanding the eukaryotic genetic code
    patent, February 2017