Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
Abstract
This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.
- Inventors:
- Issue Date:
- Research Org.:
- The Scripps Research Inst., La Jolla, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1531637
- Patent Number(s):
- 7741071
- Application Number:
- 10/580,987
- Assignee:
- The Scripps Research Institute (La Jolla, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG03-00ER45812; NIH GM66494
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2004-12-16
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Zhang, Zhiwen, Alfonta, Lital, and Schultz, Peter. Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells. United States: N. p., 2010.
Web.
Zhang, Zhiwen, Alfonta, Lital, & Schultz, Peter. Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells. United States.
Zhang, Zhiwen, Alfonta, Lital, and Schultz, Peter. Tue .
"Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells". United States. https://www.osti.gov/servlets/purl/1531637.
@article{osti_1531637,
title = {Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells},
author = {Zhang, Zhiwen and Alfonta, Lital and Schultz, Peter},
abstractNote = {This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {6}
}
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Works referencing / citing this record:
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent, May 2012
- Schultz, Peter G.; Wang, Lei; Anderson, John Christopher
- US Patent Document 8,183,012
Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
patent, October 2015
- Schultz, Peter G.; Wang, Lei; Anderson, John Christopher
- US Patent Document 9,163,271
Expanding the eukaryotic genetic code
patent, February 2017
- Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
- US Patent Document 9,580,721