Biochip reader with enhanced illumination and bioarray positioning apparatus

Yershov, Gennadiy; Alferov, Oleg; Kukhtin, Alexander

Title: Biochip reader with enhanced illumination and bioarray positioning apparatus

Abstract

A method of illumination and illumination apparatus are provided in a biochip reader. Illumination is provided by a non-collimated laser source or a light emitting diode (LED). The light is directed to opposing sides of a glass substrate by a pair of optical fiber bundles. The glass substrate carries a bioarray. Each of the optical fiber bundles are splayed out to make a fan, the fan being one fiber thick and defining a line of optical fiber faces. This process randomizes any non-uniformity in the illumination source, creating a more uniform illumination source. A respective divergent diffuser engages each row of optical fiber faces coupling and diffusing light substantially evenly through the opposing sides of the glass substrate to illuminate the bioarray supported by the glass substrate. The glass substrate functions as a secondary light guide. The divergent diffusers separate the optical fiber faces from the edges of the glass substrate, protecting the optical fibers from mechanical damage. A glass holder supports the glass substrate carrying the bioarray. The glass holder including a plastics springs member in spring contact engagement with the glass substrate for positioning said bioarray in a focal plane. Light also can be directed to opposing endsmore » « less

Inventors:: Yershov, Gennadiy; Alferov, Oleg; Kukhtin, Alexander

Issue Date:: 2007-10-30

Research Org.:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1531540

Patent Number(s):: 7288227

Application Number:: 10/619,284

Assignee:: UChicago Argonne, LLC (Chicago, IL)

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2021/6463 - {Optics}
G01N2021/6484 - {Optical fibres}
G01N21/6452 - {Individual samples arranged in a regular 2D-array, e.g. multiwell plates}

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DOE Contract Number:: W-31-109-ENG-38

Resource Type:: Patent

Resource Relation:: Patent File Date: 2003-07-14

Country of Publication:: United States

Language:: English

Subject:: 42 ENGINEERING; 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS

Citation Formats


                    Yershov, Gennadiy, Alferov, Oleg, and Kukhtin, Alexander. Biochip reader with enhanced illumination and bioarray positioning apparatus.  United States: N. p., 2007. 
        Web.

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                    Yershov, Gennadiy, Alferov, Oleg, & Kukhtin, Alexander. Biochip reader with enhanced illumination and bioarray positioning apparatus.  United States.

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                    Yershov, Gennadiy, Alferov, Oleg, and Kukhtin, Alexander. Tue .  
        "Biochip reader with enhanced illumination and bioarray positioning apparatus".  United States.  https://www.osti.gov/servlets/purl/1531540.

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@article{osti_1531540,

  title        = {Biochip reader with enhanced illumination and bioarray positioning apparatus},

  author       = {Yershov, Gennadiy and Alferov, Oleg and Kukhtin, Alexander},

  abstractNote = {A method of illumination and illumination apparatus are provided in a biochip reader. Illumination is provided by a non-collimated laser source or a light emitting diode (LED). The light is directed to opposing sides of a glass substrate by a pair of optical fiber bundles. The glass substrate carries a bioarray. Each of the optical fiber bundles are splayed out to make a fan, the fan being one fiber thick and defining a line of optical fiber faces. This process randomizes any non-uniformity in the illumination source, creating a more uniform illumination source. A respective divergent diffuser engages each row of optical fiber faces coupling and diffusing light substantially evenly through the opposing sides of the glass substrate to illuminate the bioarray supported by the glass substrate. The glass substrate functions as a secondary light guide. The divergent diffusers separate the optical fiber faces from the edges of the glass substrate, protecting the optical fibers from mechanical damage. A glass holder supports the glass substrate carrying the bioarray. The glass holder including a plastics springs member in spring contact engagement with the glass substrate for positioning said bioarray in a focal plane. Light also can be directed to opposing ends of the glass substrate by a second pair of optical fiber bundles. Also a single optical fiber bundle can be used to direct light in one side of the glass substrate or three optical fiber bundles can be used to direct light into the glass substrate. This method of illumination provides a superior signal to noise ratio as compared with conventional illumination systems.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2007},

  month        = {10}

}

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Title: Biochip reader with enhanced illumination and bioarray positioning apparatus

Abstract

Citation Formats

Light diffusing device patent, March 1988

Real time observable sample mounting fixture patent, May 2002

Object holder for thin slides patent, July 1998

Photograph slide sleeving system patent, December 1990

Specimen holder patent, July 1979

Sterilization and storage container tray patent, March 1992

Biochip detection system patent, August 2001

Device for Processing a Sample Mounted on a Surface of Support Member patent-application, July 2003

Manufacturing DNA microarrays of high spot homogeneity and reduced background signal journal, April 2001

Holder for high resolution sample imaging patent, November 1998

Holders for arrays patent-application, October 2003

Method for the determination of species in solution with an optical wave-guide patent, August 1986

Chemical level measurement device with easy action cover and single control mode selection capability patent, August 1991

Reaction cartridge and carousel for biological sample analyzer patent, June 1994

Illumination system for a film scanner patent, September 1997

Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays patent, October 1997

Clinical Spinner patent, December 1972

Devices and methods for producing microarrays of biological samples patent, June 2005

Darkfield illuminator for a microscope slide patent, September 1993

Apparatus for densitometric measurement of proteic fractions separated by electrophoresis patent, January 1984

Light diffusing device
patent, March 1988

Real time observable sample mounting fixture
patent, May 2002

Object holder for thin slides
patent, July 1998

Photograph slide sleeving system
patent, December 1990

Specimen holder
patent, July 1979

Sterilization and storage container tray
patent, March 1992

Biochip detection system
patent, August 2001

Device for Processing a Sample Mounted on a Surface of Support Member
patent-application, July 2003

Manufacturing DNA microarrays of high spot homogeneity and reduced background signal
journal, April 2001

Holder for high resolution sample imaging
patent, November 1998

Holders for arrays
patent-application, October 2003

Method for the determination of species in solution with an optical wave-guide
patent, August 1986

Chemical level measurement device with easy action cover and single control mode selection capability
patent, August 1991

Reaction cartridge and carousel for biological sample analyzer
patent, June 1994

Illumination system for a film scanner
patent, September 1997

Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
patent, October 1997

Clinical Spinner
patent, December 1972

Devices and methods for producing microarrays of biological samples
patent, June 2005

Darkfield illuminator for a microscope slide
patent, September 1993

Apparatus for densitometric measurement of proteic fractions separated by electrophoresis
patent, January 1984