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Title: Method for labeling DNA and RNA

Abstract

A method for fragmenting and labeling nucleic acids is provided. The method comprises maintaining double- and single-stranded nucleic acid molecules in an aerobic or an anaerobic atmosphere, contacting the molecules with hydrogen peroxide and radical generating coordination complexes for a time and at concentrations sufficient to produce aldehyde moieties on the molecules, reacting the aldehyde moieties with amine to produce a condensation product, and labeling the condensation product.

Inventors:
;
Issue Date:
Research Org.:
U Chicago Argonne LLC, Chicago, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531528
Patent Number(s):
7,208,269
Application Number:
10/057,753
Assignee:
U Chicago Argonne LLC (Chicago, IL)
DOE Contract Number:  
W-31-109-ENG-38
Resource Type:
Patent
Resource Relation:
Patent File Date: 2002-01-23
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Bavykin, Sergei, and Mirzabekov, Andrei D. Method for labeling DNA and RNA. United States: N. p., 2007. Web.
Bavykin, Sergei, & Mirzabekov, Andrei D. Method for labeling DNA and RNA. United States.
Bavykin, Sergei, and Mirzabekov, Andrei D. Tue . "Method for labeling DNA and RNA". United States. https://www.osti.gov/servlets/purl/1531528.
@article{osti_1531528,
title = {Method for labeling DNA and RNA},
author = {Bavykin, Sergei and Mirzabekov, Andrei D.},
abstractNote = {A method for fragmenting and labeling nucleic acids is provided. The method comprises maintaining double- and single-stranded nucleic acid molecules in an aerobic or an anaerobic atmosphere, contacting the molecules with hydrogen peroxide and radical generating coordination complexes for a time and at concentrations sufficient to produce aldehyde moieties on the molecules, reacting the aldehyde moieties with amine to produce a condensation product, and labeling the condensation product.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {4}
}

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Works referenced in this record:

Process for labeling nucleic acids and hybridization probes
patent, October 1986


Methods for immobilizing nucleic acids on a gel substrate
patent, November 1999


Column device for isolation and labeling of nucleic acids
patent, November 2004


    Works referencing / citing this record: