Method for labeling DNA and RNA
Abstract
A method for fragmenting and labeling nucleic acids is provided. The method comprises maintaining double- and single-stranded nucleic acid molecules in an aerobic or an anaerobic atmosphere, contacting the molecules with hydrogen peroxide and radical generating coordination complexes for a time and at concentrations sufficient to produce aldehyde moieties on the molecules, reacting the aldehyde moieties with amine to produce a condensation product, and labeling the condensation product.
- Inventors:
- Issue Date:
- Research Org.:
- U Chicago Argonne LLC, Chicago, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1531528
- Patent Number(s):
- 7,208,269
- Application Number:
- 10/057,753
- Assignee:
- U Chicago Argonne LLC (Chicago, IL)
- DOE Contract Number:
- W-31-109-ENG-38
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2002-01-23
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Bavykin, Sergei, and Mirzabekov, Andrei D. Method for labeling DNA and RNA. United States: N. p., 2007.
Web.
Bavykin, Sergei, & Mirzabekov, Andrei D. Method for labeling DNA and RNA. United States.
Bavykin, Sergei, and Mirzabekov, Andrei D. Tue .
"Method for labeling DNA and RNA". United States. https://www.osti.gov/servlets/purl/1531528.
@article{osti_1531528,
title = {Method for labeling DNA and RNA},
author = {Bavykin, Sergei and Mirzabekov, Andrei D.},
abstractNote = {A method for fragmenting and labeling nucleic acids is provided. The method comprises maintaining double- and single-stranded nucleic acid molecules in an aerobic or an anaerobic atmosphere, contacting the molecules with hydrogen peroxide and radical generating coordination complexes for a time and at concentrations sufficient to produce aldehyde moieties on the molecules, reacting the aldehyde moieties with amine to produce a condensation product, and labeling the condensation product.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {4}
}
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Works referenced in this record:
Process for labeling nucleic acids and hybridization probes
patent, October 1986
- Sheldon, III, Edward L.; Levenson, Corey H.; Mullis, Kary B.
- US Patent Document 4,617,261
Methods for immobilizing nucleic acids on a gel substrate
patent, November 1999
- Mirzabekov, Andrei; Proudnikov, Dimitri Y.; Timofeev, Edward N.
- US Patent Document 5,981,734
Column device for isolation and labeling of nucleic acids
patent, November 2004
- Bavykin, Sergei G.; Akowski, James P.; Zakhariev, Vladimir M.
- US Patent Document 6,818,398
Works referencing / citing this record:
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- Bitner, Rex M.; Mandreker, Michelle M.; Brisco, Paula R.
- US Patent Document 8,658,360
Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
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- Bitner, Rex M.
- US Patent Document 8,039,613
Nucleic acid purification with a binding matrix
patent, October 2011
- Bitner, Rex M.; Mandrekar, Michelle A.; Brisco, Paula R.
- US Patent Document 8,030,034
Methods of optimal purification of nucleic acids and kit for use in performing such methods
patent, July 2012
- Bitner, Rex M.
- US Patent Document 8,222,397
Universal nucleic acids sample preparation method for cells, spores and their mixture
patent, January 2011
- Bavykin, Sergei G.
- US Patent Document 7,871,764
Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
patent, August 2013
- Bitner, Rex M.
- US Patent Document 8,519,119