Method for labeling DNA and RNA
Abstract
A method for fragmenting and labeling nucleic acids is provided. The method comprises maintaining double- and single-stranded nucleic acid molecules in an aerobic or an anaerobic atmosphere, contacting the molecules with hydrogen peroxide and radical generating coordination complexes for a time and at concentrations sufficient to produce aldehyde moieties on the molecules, reacting the aldehyde moieties with amine to produce a condensation product, and labeling the condensation product.
- Inventors:
- Issue Date:
- Research Org.:
- U Chicago Argonne LLC, Chicago, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1531528
- Patent Number(s):
- 7208269
- Application Number:
- 10/057,753
- Assignee:
- U Chicago Argonne LLC (Chicago, IL)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
- DOE Contract Number:
- W-31-109-ENG-38
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2002-01-23
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Bavykin, Sergei, and Mirzabekov, Andrei D. Method for labeling DNA and RNA. United States: N. p., 2007.
Web.
Bavykin, Sergei, & Mirzabekov, Andrei D. Method for labeling DNA and RNA. United States.
Bavykin, Sergei, and Mirzabekov, Andrei D. Tue .
"Method for labeling DNA and RNA". United States. https://www.osti.gov/servlets/purl/1531528.
@article{osti_1531528,
title = {Method for labeling DNA and RNA},
author = {Bavykin, Sergei and Mirzabekov, Andrei D.},
abstractNote = {A method for fragmenting and labeling nucleic acids is provided. The method comprises maintaining double- and single-stranded nucleic acid molecules in an aerobic or an anaerobic atmosphere, contacting the molecules with hydrogen peroxide and radical generating coordination complexes for a time and at concentrations sufficient to produce aldehyde moieties on the molecules, reacting the aldehyde moieties with amine to produce a condensation product, and labeling the condensation product.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {4}
}
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Works referencing / citing this record:
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- Bitner, Rex M.; Mandreker, Michelle M.; Brisco, Paula R.
- US Patent Document 8,658,360
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- Bitner, Rex M.
- US Patent Document 8,039,613
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- Bitner, Rex M.; Mandrekar, Michelle; Brisco, Paula R.
- US Patent Document 8,030,034
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- Bitner, Rex M.
- US Patent Document 8,222,397
Universal nucleic acids sample preparation method for cells, spores and their mixture
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- US Patent Document 7,871,764
Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
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- Bitner, Rex M.
- US Patent Document 8,519,119