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Title: Method for labeling DNA and RNA

Abstract

A method for fragmenting and labeling nucleic acids is provided. The method comprises maintaining double- and single-stranded nucleic acid molecules in an aerobic or an anaerobic atmosphere, contacting the molecules with hydrogen peroxide and radical generating coordination complexes for a time and at concentrations sufficient to produce aldehyde moieties on the molecules, reacting the aldehyde moieties with amine to produce a condensation product, and labeling the condensation product.

Inventors:
;
Issue Date:
Research Org.:
U Chicago Argonne LLC, Chicago, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531528
Patent Number(s):
7208269
Application Number:
10/057,753
Assignee:
U Chicago Argonne LLC (Chicago, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-31-109-ENG-38
Resource Type:
Patent
Resource Relation:
Patent File Date: 2002-01-23
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Bavykin, Sergei, and Mirzabekov, Andrei D. Method for labeling DNA and RNA. United States: N. p., 2007. Web.
Bavykin, Sergei, & Mirzabekov, Andrei D. Method for labeling DNA and RNA. United States.
Bavykin, Sergei, and Mirzabekov, Andrei D. Tue . "Method for labeling DNA and RNA". United States. https://www.osti.gov/servlets/purl/1531528.
@article{osti_1531528,
title = {Method for labeling DNA and RNA},
author = {Bavykin, Sergei and Mirzabekov, Andrei D.},
abstractNote = {A method for fragmenting and labeling nucleic acids is provided. The method comprises maintaining double- and single-stranded nucleic acid molecules in an aerobic or an anaerobic atmosphere, contacting the molecules with hydrogen peroxide and radical generating coordination complexes for a time and at concentrations sufficient to produce aldehyde moieties on the molecules, reacting the aldehyde moieties with amine to produce a condensation product, and labeling the condensation product.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {4}
}

Works referenced in this record:

Process for labeling nucleic acids and hybridization probes
patent, October 1986


Methods for immobilizing nucleic acids on a gel substrate
patent, November 1999


Automated sequencing of fluorescently labelled DNA by chemical degradation
journal, January 1990


Column device for isolation and labeling of nucleic acids
patent, November 2004


    Works referencing / citing this record: