Methods and compositions for amplifying DNA clone copy number

Title: Methods and compositions for amplifying DNA clone copy number

Abstract

A method for retrofitting DNA in a single-copy or high-copy vector, such as a fosmid or BAC, whereby an artificial transposon is used to introduce a conditional multi-copy origin of replication (“ori”) into the DNA in said vector. Following random in vitro or in vivo transposition of the ori-containing transposon into DNA in the single-copy or low-copy vector, the resulting insertion clones are introduced into a special host strain that contains a gene which encodes a polypeptide required for replication from the multi-copy ori. However, since the gene for this polypeptide is expressed from a tightly-regulated inducible promoter, the polypeptide is not expressed in the absence of inducer. On addition of inducer to the culture medium, the host cell synthesizes the polypeptide, which in turn activates replication from the multi-copy ori, thereby increasing the amount of clone DNA synthesized by the cell.

Inventors:: Jendrisak, Jerome J.; Hoffman, Leslie M.; Fiandt, Michael J.; Haskins, Darin

Issue Date:: 2006-11-21

Research Org.:: Epicentre Technologies Corp., Madison, WI (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1531519

Patent Number(s):: 7,138,267

Application Number:: 10/116,886

Assignee:: Epicentre Technologies Corporation (Madison, WI)

DOE Contract Number:: FG02-00ER83003

Resource Type:: Patent

Resource Relation:: Patent File Date: 2002-04-04

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Jendrisak, Jerome J., Hoffman, Leslie M., Fiandt, Michael J., and Haskins, Darin. Methods and compositions for amplifying DNA clone copy number.  United States: N. p., 2006. 
        Web.

Copy to clipboard


                    Jendrisak, Jerome J., Hoffman, Leslie M., Fiandt, Michael J., & Haskins, Darin. Methods and compositions for amplifying DNA clone copy number.  United States.

Copy to clipboard


                    Jendrisak, Jerome J., Hoffman, Leslie M., Fiandt, Michael J., and Haskins, Darin. Tue .  
        "Methods and compositions for amplifying DNA clone copy number".  United States.  https://www.osti.gov/servlets/purl/1531519.

Copy to clipboard


                    
@article{osti_1531519,

  title        = {Methods and compositions for amplifying DNA clone copy number},

  author       = {Jendrisak, Jerome J. and Hoffman, Leslie M. and Fiandt, Michael J. and Haskins, Darin},

  abstractNote = {A method for retrofitting DNA in a single-copy or high-copy vector, such as a fosmid or BAC, whereby an artificial transposon is used to introduce a conditional multi-copy origin of replication (“ori”) into the DNA in said vector. Following random in vitro or in vivo transposition of the ori-containing transposon into DNA in the single-copy or low-copy vector, the resulting insertion clones are introduced into a special host strain that contains a gene which encodes a polypeptide required for replication from the multi-copy ori. However, since the gene for this polypeptide is expressed from a tightly-regulated inducible promoter, the polypeptide is not expressed in the absence of inducer. On addition of inducer to the culture medium, the host cell synthesizes the polypeptide, which in turn activates replication from the multi-copy ori, thereby increasing the amount of clone DNA synthesized by the cell.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2006},

  month        = {11}

}

Copy to clipboard

Patent:

View Patent

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Circular DNA expression cassettes for in vivo gene transfer
patent, November 2000

Crouzet, Joel; Scherman, Daniel; Cameron, Beatrice
US Patent Document 6,143,530
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6143530

Method for making insertional mutations using a Tn5 synaptic complex
patent, December 2000

Reznikoff, William S.; Goryshin, Igor Yu
US Patent Document 6,159,736
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6159736

High throughput DNA sequencing vector
patent, July 2001

Chumakov, Ilya; Tanaka, Hiroaki
US Patent Document 6,258,571
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6258571

System for in vitro transposition
patent, September 1999

Reznikoff, William S.; Goryshin, Igor Yu; York, Dona L.
US Patent Document 5,948,622
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5948622

AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques
patent, July 1991

Lai, Jiunu S.; Lee, Jar-How; Lin, Yun-Long
US Patent Document 5,028,530
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5028530

Method capable of increasing competency of bacterial cell transformation
patent, August 2001

Donahue, Jr., Robert A.; Bebee, Robert L.
US Patent Document 6,274,369
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6274369

Trichothecne-Resistant transgenic plants
patent, February 2002

Hohn, Thomas M.; Peters, Cheryl L.; Salmeron, John M.
US Patent Document 6,346,655
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6346655

Expression vectors based on RK2 and TOL plasmids
patent, July 2001

Blatny, Janet M.; Karunakaran, Ponniah; Valla, Svein
US Patent Document 6,258,565
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6258565

Method for making insertional mutations using a Tn5 synaptic complex
patent, September 2001

Goryshin, Igor Yu; Reznikoff, William S.
US Patent Document 6,294,385
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6294385

High throughput DNA sequencing vector
patent, February 2000

Chumakov, Ilya; Tanaka, Hiroaki
US Patent Document 6,022,716
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6022716

Conditionally amplifiable BAC vector
patent, February 1999

Szybalski, Waclaw T.
US Patent Document 5,874,259
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5874259

Transferrin receptor subunit proteins of Neisseria meningitidis
patent, December 2001

Jacobs, Eric; Legrain, Michele; Mazarin, Veronique
US Patent Document 6,326,350
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6326350

Binary BAC vector
patent, November 1999

Hamilton, Carol M.
US Patent Document 5,977,439
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5977439

Binary BAC vector
patent, March 1998

Hamilton, Carol M.
US Patent Document 5,733,744
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5733744

Highly regulable promoter for heterologous gene expression
patent, February 2000

De Lencastre, Herminia; De Sa-Nogueira, Isabel
US Patent Document 6,030,807
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6030807

Works referencing / citing this record:

Methods and systems for processing polynucleotides
patent, February 2019

Hindson, Benjamin J.; Hindson, Christopher M.; Schnall-Levin, Michael
US Patent Document 10,208,343
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10208343

Sample preparation on a solid support
patent, June 2017

Gormley, Niall Anthony; Smith, Geoffrey Paul
US Patent Document 9,683,230
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=9683230

Linking sequence reads using paired code tags
patent, September 2014

Steemers, Frank J.; Gunderson, Kevin L.; Royce, Thomas
US Patent Document 8,829,171
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=8829171

Methods and systems for processing polynucleotides
patent, August 2018

Hindson, Benjamin J.; Hindson, Christopher M.; Schnall-Levin, Michael
US Patent Document 10,041,116
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10041116

Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
patent, March 2019

Hardenbol, Paul; Patel, Pranav; Hindson, Benjamin J.
US Patent Document 10,221,436
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10221436

Transposition of native chromatin for personal epigenomics
patent, December 2018

Giresi, Paul; Buenrostro, Jason D.; Chang, Howard Y.
US Patent Document 10,150,995
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10150995

Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
patent, December 2018

Bharadwaj, Rajiv; Ness, Kevin D.; Mitra, Debkishore
US Patent Document 10,150,117
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10150117

Sample preparation on a solid support
patent, August 2018

Gormley, Niall Anthony; Smith, Geoffrey Paul
US Patent Document 10,041,066
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10041066

Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
patent, September 2018

Bharadwaj, Rajiv; Ness, Kevin D.; Mitra, Debkishore
US Patent Document 10,071,377
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10071377

Capsule array devices and methods of use
patent, August 2018

Hindson, Benjamin J.; Saxonov, Serge; Schnall-Levin, Michael
US Patent Document 10,053,723
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10053723

Methods and systems for processing polynucleotides
patent, March 2019

Hindson, Benjamin J.; Saxonov, Serge; Ness, Kevin D.
US Patent Document 10,227,648
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10227648

Transposition of native chromatin for personal epigenomics
patent, August 2018

Giresi, Paul; Buenrostro, Jason D.; Chang, Howard Y.
US Patent Document 10,059,989
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10059989

Methods and systems for processing polynucleotides
patent, July 2018

Hindson, Benjamin J.; Hindson, Christopher M.; Schnall-Levin, Michael
US Patent Document 10,030,267
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10030267

Linking sequence reads using paired code tags
patent, July 2015

Steemers, Frank J.; Gunderson, Kevin L.; Royce, Thomas
US Patent Document 9,074,251
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=9074251

Partitioning and processing of analytes and other species
patent, December 2018

Hindson, Benjamin J.; Saxonov, Serge; Ness, Kevin D.
US Patent Document 10,150,963
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10150963

Partitioning and processing of analytes and other species
patent, December 2018

Hindson, Benjamin J.; Saxonov, Serge; Ness, Kevin D.
US Patent Document 10,150,964
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10150964

Compositions and methods for sample processing
patent, March 2019

Hindson, Benjamin J.; Hindson, Christopher M.; Schnall-Levin, Michael
US Patent Document 10,221,442
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=10221442

Similar records in DOE Patents and OSTI.GOV collections:

Phosphorescent compositions, methods of making the compositions, and methods of using the compositions

Patent Jia, Weiyi; Wang, Xiaojun; Yen, William; ...

Compositions, methods of making compositions, materials including compositions, crayons including compositions, paint including compositions, ink including compositions, waxes including compositions, polymers including compositions, vesicles including the compositions, methods of making each, and the like are disclosed.
Full Text Available
Phosphorescent compositions, methods of making the compositions, and methods of using the compositions

Patent Jia, Weiyi; Wang, Xiaojun; Jia, George D.; ...

Compositions, methods of making compositions, materials including compositions, crayons including compositions, paint including compositions, ink including compositions, waxes including compositions, polymers including compositions, vesicles including the compositions, methods of making each, and the like are disclosed.
Full Text Available
Chemical compositions, methods of making the chemical compositions, and structures made from the chemical compositions

Patent Yang, Lei; Cheng, Zhe; Liu, Ze; ...

Embodiments of the present disclosure include chemical compositions, structures, anodes, cathodes, electrolytes for solid oxide fuel cells, solid oxide fuel cells, fuel cells, fuel cell membranes, separation membranes, catalytic membranes, sensors, coatings for electrolytes, electrodes, membranes, and catalysts, and the like, are disclosed.
Full Text Available
Coating compositions for electrode compositions and their methods of making

Patent Parans Paranthaman, Mariappan; Bridges, Craig A.; Yoon, Sukeun; ...

Compositions and methods of making are provided for coated electrodes and batteries comprising the same. The compositions may comprise a base composition having an active material selected from the group consisting of LiCoO.sub.2, LiMn.sub.2O.sub.4, Li.sub.2MnO.sub.3, LiNiO.sub.2, LiMn.sub.1.5Ni.sub.0.5O.sub.4, LiFePO.sub.4, Li.sub.2FePO.sub.4F, Li.sub.3CoNiMnO.sub.6, Li(Li.sub.aNi.sub.xMn.sub.yCo.sub.z)O.sub.2, and mixtures thereof. The compositions may also comprise a coating composition that covers at least a portion of the base composition, wherein the coating composition comprises a non-metal or metalloid element. The methods of making comprise providing the base composition and a doped carbon coating composition, and mixing the coating composition with the base electrode composition at an elevatedmore »« less
Full Text Available
Methods of making copper selenium precursor compositions with a targeted copper selenide content and precursor compositions and thin films resulting therefrom

Patent Curtis, Calvin J [Lakewood, CO]; Miedaner, Alexander [Boulder, CO]; van Hest, Marinus Franciscus Antonius Maria (Lakewood, CO); ...

Precursor compositions containing copper and selenium suitable for deposition on a substrate to form thin films suitable for semi-conductor applications. Methods of forming the precursor compositions using primary amine solvents and methods of forming the thin films wherein the selection of temperature and duration of heating controls the formation of a targeted species of copper selenide.
Full Text Available

Similar Records

Title: Methods and compositions for amplifying DNA clone copy number

Abstract

Citation Formats

Circular DNA expression cassettes for in vivo gene transfer patent, November 2000

Method for making insertional mutations using a Tn5 synaptic complex patent, December 2000

High throughput DNA sequencing vector patent, July 2001

System for in vitro transposition patent, September 1999

AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques patent, July 1991

Method capable of increasing competency of bacterial cell transformation patent, August 2001

Trichothecne-Resistant transgenic plants patent, February 2002

Expression vectors based on RK2 and TOL plasmids patent, July 2001

Method for making insertional mutations using a Tn5 synaptic complex patent, September 2001

High throughput DNA sequencing vector patent, February 2000

Conditionally amplifiable BAC vector patent, February 1999

Transferrin receptor subunit proteins of Neisseria meningitidis patent, December 2001

Binary BAC vector patent, November 1999

Binary BAC vector patent, March 1998

Highly regulable promoter for heterologous gene expression patent, February 2000

Methods and systems for processing polynucleotides patent, February 2019

Sample preparation on a solid support patent, June 2017

Linking sequence reads using paired code tags patent, September 2014

Methods and systems for processing polynucleotides patent, August 2018

Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same patent, March 2019

Transposition of native chromatin for personal epigenomics patent, December 2018

Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same patent, December 2018

Sample preparation on a solid support patent, August 2018

Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same patent, September 2018

Capsule array devices and methods of use patent, August 2018

Methods and systems for processing polynucleotides patent, March 2019

Transposition of native chromatin for personal epigenomics patent, August 2018

Methods and systems for processing polynucleotides patent, July 2018

Linking sequence reads using paired code tags patent, July 2015

Partitioning and processing of analytes and other species patent, December 2018

Partitioning and processing of analytes and other species patent, December 2018

Compositions and methods for sample processing patent, March 2019

Circular DNA expression cassettes for in vivo gene transfer
patent, November 2000

Method for making insertional mutations using a Tn5 synaptic complex
patent, December 2000

High throughput DNA sequencing vector
patent, July 2001

System for in vitro transposition
patent, September 1999

AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques
patent, July 1991

Method capable of increasing competency of bacterial cell transformation
patent, August 2001

Trichothecne-Resistant transgenic plants
patent, February 2002

Expression vectors based on RK2 and TOL plasmids
patent, July 2001

Method for making insertional mutations using a Tn5 synaptic complex
patent, September 2001

High throughput DNA sequencing vector
patent, February 2000

Conditionally amplifiable BAC vector
patent, February 1999

Transferrin receptor subunit proteins of Neisseria meningitidis
patent, December 2001

Binary BAC vector
patent, November 1999

Binary BAC vector
patent, March 1998

Highly regulable promoter for heterologous gene expression
patent, February 2000

Methods and systems for processing polynucleotides
patent, February 2019

Sample preparation on a solid support
patent, June 2017

Linking sequence reads using paired code tags
patent, September 2014

Methods and systems for processing polynucleotides
patent, August 2018

Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
patent, March 2019

Transposition of native chromatin for personal epigenomics
patent, December 2018

Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
patent, December 2018

Sample preparation on a solid support
patent, August 2018

Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
patent, September 2018

Capsule array devices and methods of use
patent, August 2018

Methods and systems for processing polynucleotides
patent, March 2019

Transposition of native chromatin for personal epigenomics
patent, August 2018

Methods and systems for processing polynucleotides
patent, July 2018

Linking sequence reads using paired code tags
patent, July 2015

Partitioning and processing of analytes and other species
patent, December 2018

Partitioning and processing of analytes and other species
patent, December 2018

Compositions and methods for sample processing
patent, March 2019