Column device for isolation and labeling of nucleic acids
Abstract
A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Chicago, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1531485
- Patent Number(s):
- 6818398
- Application Number:
- 09/751,654
- Assignee:
- The University of Chicago (Chicago, IL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-31-109-ENG-38
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2000-12-29
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., and Mirzabekov, Andrei. Column device for isolation and labeling of nucleic acids. United States: N. p., 2004.
Web.
Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., & Mirzabekov, Andrei. Column device for isolation and labeling of nucleic acids. United States.
Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., and Mirzabekov, Andrei. Tue .
"Column device for isolation and labeling of nucleic acids". United States. https://www.osti.gov/servlets/purl/1531485.
@article{osti_1531485,
title = {Column device for isolation and labeling of nucleic acids},
author = {Bavykin, Sergei G. and Akowski, James P. and Zakhariev, Vladimir M. and Mirzabekov, Andrei},
abstractNote = {A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {11}
}