Column device for isolation and labeling of nucleic acids

Bavykin, Sergei G.; Akowski, James P.; Zakhariev, Vladimir M.; Mirzabekov, Andrei

Title: Column device for isolation and labeling of nucleic acids

Abstract

A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.

Inventors:: Bavykin, Sergei G.; Akowski, James P.; Zakhariev, Vladimir M.; Mirzabekov, Andrei

Issue Date:: 2004-11-16

Research Org.:: Univ. of Chicago, IL (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1531485

Patent Number(s):: 6818398

Application Number:: 09/751,654

Assignee:: The University of Chicago (Chicago, IL)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
C07H21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q2563/113 - the label being electroactive, e.g. redox labels
C12Q2563/107 - fluorescence
C12Q1/6816 - characterised by the detection means

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/101 - {by chromatography, e.g. electrophoresis, ion-exchange, reverse phase}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
B01J20/283 - based on silica

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DOE Contract Number:: W-31-109-ENG-38

Resource Type:: Patent

Resource Relation:: Patent File Date: 2000-12-29

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats


                    Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., and Mirzabekov, Andrei. Column device for isolation and labeling of nucleic acids.  United States: N. p., 2004. 
        Web.

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                    Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., & Mirzabekov, Andrei. Column device for isolation and labeling of nucleic acids.  United States.

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                    Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., and Mirzabekov, Andrei. Tue .  
        "Column device for isolation and labeling of nucleic acids".  United States.  https://www.osti.gov/servlets/purl/1531485.

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@article{osti_1531485,

  title        = {Column device for isolation and labeling of nucleic acids},

  author       = {Bavykin, Sergei G. and Akowski, James P. and Zakhariev, Vladimir M. and Mirzabekov, Andrei},

  abstractNote = {A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2004},

  month        = {11}

}

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Works referenced in this record:

Methods for immobilizing nucleic acids on a gel substrate
patent, November 1999

Mirzabekov, Andrei; Proudnikov, Dimitri Y.; Timofeev, Edward N.
US Patent Document 5,981,734
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Method of isolating RNA
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Fluorescent labelling of closely-spaced aldehydes induced in DNA by bleomycin-Fe(III)
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https://doi.org/10.1093/nar/24.20.4092

Chemical Methods of DNA and RNA Fluorescent Labeling
journal, November 1996

Proudnikov, D.; Mirzabekov, A.
Nucleic Acids Research, Vol. 24, Issue 22
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Device and process for isolating nucleic acids from cell suspension
patent, July 1997

Henco, Karsten; Colpan, Metin; Feuser, Petra
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Works referencing / citing this record:

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Bavykin, Sergei G.; Mirzabekov, Andrei
US Patent Document 7,208,269
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7208269

Labelling reagents having a pyridine nucleus bearing a diazomethyl function, process for synthesis of such reagents and processes for detection of biological molecules
patent, February 2016

Laurent, Alain; Laayoun, Ali; Kotera, Mitsuharu
US Patent Document 9,266,902
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=9266902

Labeling reagents, methods for the synthesis of such reagents and methods for the detection of biological molecules
patent, April 2010

Laayoun, Ali; Bernal-Mendez, Eloy
US Patent Document 7,691,635
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7691635

Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling
patent, June 2006

Bourget, Cecile; Kotera, Mitsuharu; Lhomme, Jean
US Patent Document 7,060,441
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7060441

Marking reagents bearing diazo and nitro functions, methods for the synthesis of such reagents and methods for detecting biological molecules
patent, November 2012

Laurent, Alain; Laayoun, Ali; Kotera, Mitsuharu
US Patent Document 8,309,695
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=8309695

Universal nucleic acids sample preparation method for cells, spores and their mixture
patent, January 2011

Bavykin, Sergei G.
US Patent Document 7,871,764
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7871764

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Similar Records

Title: Column device for isolation and labeling of nucleic acids

Abstract

Citation Formats

Methods for immobilizing nucleic acids on a gel substrate patent, November 1999

Method of isolating RNA patent, April 2001

Fluorescent labelling of closely-spaced aldehydes induced in DNA by bleomycin-Fe(III) journal, January 1999

A new approach for the electrophoretic detection of apoptosis journal, October 1996

Chemical Methods of DNA and RNA Fluorescent Labeling journal, November 1996

Device and process for isolating nucleic acids from cell suspension patent, July 1997

Method for labeling DNA and RNA patent, April 2007

Labelling reagents having a pyridine nucleus bearing a diazomethyl function, process for synthesis of such reagents and processes for detection of biological molecules patent, February 2016

Labeling reagents, methods for the synthesis of such reagents and methods for the detection of biological molecules patent, April 2010

Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling patent, June 2006

Marking reagents bearing diazo and nitro functions, methods for the synthesis of such reagents and methods for detecting biological molecules patent, November 2012

Universal nucleic acids sample preparation method for cells, spores and their mixture patent, January 2011

Methods for immobilizing nucleic acids on a gel substrate
patent, November 1999

Method of isolating RNA
patent, April 2001

Fluorescent labelling of closely-spaced aldehydes induced in DNA by bleomycin-Fe(III)
journal, January 1999

A new approach for the electrophoretic detection of apoptosis
journal, October 1996

Chemical Methods of DNA and RNA Fluorescent Labeling
journal, November 1996

Device and process for isolating nucleic acids from cell suspension
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Method for labeling DNA and RNA
patent, April 2007

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Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling
patent, June 2006

Marking reagents bearing diazo and nitro functions, methods for the synthesis of such reagents and methods for detecting biological molecules
patent, November 2012

Universal nucleic acids sample preparation method for cells, spores and their mixture
patent, January 2011