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Title: Column device for isolation and labeling of nucleic acids

Abstract

A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.

Inventors:
; ; ;
Issue Date:
Research Org.:
Univ. of Chicago, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531485
Patent Number(s):
6818398
Application Number:
09/751,654
Assignee:
The University of Chicago (Chicago, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-31-109-ENG-38
Resource Type:
Patent
Resource Relation:
Patent File Date: 2000-12-29
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., and Mirzabekov, Andrei. Column device for isolation and labeling of nucleic acids. United States: N. p., 2004. Web.
Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., & Mirzabekov, Andrei. Column device for isolation and labeling of nucleic acids. United States.
Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., and Mirzabekov, Andrei. Tue . "Column device for isolation and labeling of nucleic acids". United States. https://www.osti.gov/servlets/purl/1531485.
@article{osti_1531485,
title = {Column device for isolation and labeling of nucleic acids},
author = {Bavykin, Sergei G. and Akowski, James P. and Zakhariev, Vladimir M. and Mirzabekov, Andrei},
abstractNote = {A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {11}
}

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