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Title: Column device for isolation and labeling of nucleic acids

Abstract

A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.

Inventors:
; ; ;
Issue Date:
Research Org.:
Univ. of Chicago, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531485
Patent Number(s):
6818398
Application Number:
09/751,654
Assignee:
The University of Chicago (Chicago, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-31-109-ENG-38
Resource Type:
Patent
Resource Relation:
Patent File Date: 2000-12-29
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., and Mirzabekov, Andrei. Column device for isolation and labeling of nucleic acids. United States: N. p., 2004. Web.
Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., & Mirzabekov, Andrei. Column device for isolation and labeling of nucleic acids. United States.
Bavykin, Sergei G., Akowski, James P., Zakhariev, Vladimir M., and Mirzabekov, Andrei. Tue . "Column device for isolation and labeling of nucleic acids". United States. https://www.osti.gov/servlets/purl/1531485.
@article{osti_1531485,
title = {Column device for isolation and labeling of nucleic acids},
author = {Bavykin, Sergei G. and Akowski, James P. and Zakhariev, Vladimir M. and Mirzabekov, Andrei},
abstractNote = {A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {11}
}

Works referenced in this record:

Methods for immobilizing nucleic acids on a gel substrate
patent, November 1999


Method of isolating RNA
patent, April 2001


Fluorescent labelling of closely-spaced aldehydes induced in DNA by bleomycin-Fe(III)
journal, January 1999


A new approach for the electrophoretic detection of apoptosis
journal, October 1996


Chemical Methods of DNA and RNA Fluorescent Labeling
journal, November 1996


Device and process for isolating nucleic acids from cell suspension
patent, July 1997


    Works referencing / citing this record:

    Method for labeling DNA and RNA
    patent, April 2007


    Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling
    patent, June 2006