Biological conjugates of fluorescent rhodol dyes
Abstract
Novel fluorescent dyes based on the rhodol structure are provided. The new reagents contain functional groups capable of forming a stable fluorescent product with functional groups typically found in biomolecules or polymers including amines, phenols, thiols, acids, aldehydes and ketones. Reactive groups in the rhodol dyes include activated esters, isothiocyanates, amines, hydrazines, halides, acids, azides, maleimides, aldehydes, alcohols, acrylamides and haloacetamides. The products are detected by their absorbance or fluorescence properties. The spectral properties of the fluorescent dyes are sufficiently similar in wavelengths and intensity to fluorescein or rhodamine derivatives as to permit use of the same equipment. The dyes, however, show less spectral sensitivity to pH in the physiological range than does fluorescein, have higher solubility in non-polar solvents and have improved photostability and quantum yields.
- Inventors:
- Issue Date:
- Research Org.:
- Molecular Probes, Inc., Eugene, OR (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1531413
- Patent Number(s):
- 5442045
- Application Number:
- 08/028,319
- Assignee:
- Molecular Probes, Inc. (Eugene, OR)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07D - HETEROCYCLIC COMPOUNDS
C - CHEMISTRY C09 - DYES C09B - ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES {, e.g. PIGMENTS},
- DOE Contract Number:
- FG06-88ER60684
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 1993-03-08
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Haugland, Richard P., and Whitaker, James E. Biological conjugates of fluorescent rhodol dyes. United States: N. p., 1995.
Web.
Haugland, Richard P., & Whitaker, James E. Biological conjugates of fluorescent rhodol dyes. United States.
Haugland, Richard P., and Whitaker, James E. Tue .
"Biological conjugates of fluorescent rhodol dyes". United States. https://www.osti.gov/servlets/purl/1531413.
@article{osti_1531413,
title = {Biological conjugates of fluorescent rhodol dyes},
author = {Haugland, Richard P. and Whitaker, James E.},
abstractNote = {Novel fluorescent dyes based on the rhodol structure are provided. The new reagents contain functional groups capable of forming a stable fluorescent product with functional groups typically found in biomolecules or polymers including amines, phenols, thiols, acids, aldehydes and ketones. Reactive groups in the rhodol dyes include activated esters, isothiocyanates, amines, hydrazines, halides, acids, azides, maleimides, aldehydes, alcohols, acrylamides and haloacetamides. The products are detected by their absorbance or fluorescence properties. The spectral properties of the fluorescent dyes are sufficiently similar in wavelengths and intensity to fluorescein or rhodamine derivatives as to permit use of the same equipment. The dyes, however, show less spectral sensitivity to pH in the physiological range than does fluorescein, have higher solubility in non-polar solvents and have improved photostability and quantum yields.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {8}
}
Works referenced in this record:
Analysis of glycoforms present in two mouse IgG2a monoclonal antibody preparations
journal, November 1992
- Coco-Martin, JoséM.; Brunink, Freek; van der Velden-de Groot, Tiny A. M.
- Journal of Immunological Methods, Vol. 155, Issue 2
Fluorescent rhodol derivatives: Versatile, photostable labels and tracers
journal, December 1992
- Whitaker, James E.; Haugland, Rosaria P.; Ryan, Diane
- Analytical Biochemistry, Vol. 207, Issue 2