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Title: Methods for high-throughput labelling and detection of biological features in situ using microscopy

Abstract

Methods of labelling one or more subcellular components (e.g., an organelle and/or subcellular region) in vivo are provided. Methods of labelling a protein in vivo are provided. Methods of determining a nucleic acid sequence in situ are also provided.

Inventors:
; ;
Issue Date:
Research Org.:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531401
Patent Number(s):
10179932
Application Number:
15/325,577
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015-07-10
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Church, George M., Lee, Je-Hyuk, and Daugharthy, Evan R.. Methods for high-throughput labelling and detection of biological features in situ using microscopy. United States: N. p., 2019. Web.
Church, George M., Lee, Je-Hyuk, & Daugharthy, Evan R.. Methods for high-throughput labelling and detection of biological features in situ using microscopy. United States.
Church, George M., Lee, Je-Hyuk, and Daugharthy, Evan R.. Tue . "Methods for high-throughput labelling and detection of biological features in situ using microscopy". United States. https://www.osti.gov/servlets/purl/1531401.
@article{osti_1531401,
title = {Methods for high-throughput labelling and detection of biological features in situ using microscopy},
author = {Church, George M. and Lee, Je-Hyuk and Daugharthy, Evan R.},
abstractNote = {Methods of labelling one or more subcellular components (e.g., an organelle and/or subcellular region) in vivo are provided. Methods of labelling a protein in vivo are provided. Methods of determining a nucleic acid sequence in situ are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {1}
}

Works referenced in this record:

RNA sequencing in situ
journal, June 2014


Highly Multiplexed Subcellular RNA Sequencing in Situ
journal, February 2014


Automated Method for Detecting Cancers and High Grade Hyperplasias
patent-application, August 2009


Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays
journal, June 2000


Isolation of three homologous AP1-like MADS-box genes in crocus (Crocus sativus L.) and characterization of their expression
journal, May 2004


Single-cell RNA-seq: advances and future challenges
journal, July 2014


The rapid detection of unknown mutations in nucleic acids
journal, October 1993


High-resolution in situ hybridization to whole-mount zebrafish embryos
journal, December 2007


Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
journal, April 2005


Methods of amplifying and sequencing nucleic acids
patent, January 2008


Protein targeting to subcellular organelles via mRNA localization
journal, February 2013


Rolling circle amplification-RACE: a method for simultaneous isolation of 5′ and 3′ cDNA ends from amplified cDNA templates
journal, July 2006


Here, there, everywhere: mRNA localization in budding yeast
journal, August 2014


mRNA on the Move: The Road to Its Biological Destiny
journal, July 2013


Spatial expression of the genome: the signal hypothesis at forty
journal, April 2011


Cis-acting determinants of asymmetric, cytoplasmic RNA transport
journal, May 2007


Identification of RNA-protein interaction networks using PAR-CLIP: Identification of RNA-protein interaction networks
journal, December 2011


Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome
journal, September 2005


Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules
journal, July 2001


Characterization of Genes Encoding Poly(A) Polymerases in Plants: Evidence for Duplication and Functional Specialization
journal, November 2009