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Title: Method of nucleic acid cassette assembly

Abstract

Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Rational methods may be used to design the synthetic genome (e.g., to establish a minimal genome and/or to optimize the function of genes within a genome, such as by mutating or rearranging the order of the genes). Synthetic genomes of the invention may be introduced into vesicles (e.g., bacterial cells from which part or all of the resident genome has been removed, or synthetic vesicles) to generate synthetic cells. Synthetic genomes or synthetic cells may be used for a variety of purposes, including the generation of synthetic fuels, such as hydrogen or ethanol.

Inventors:
; ; ;
Issue Date:
Research Org.:
Synthetic Genomics, Inc., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531354
Patent Number(s):
10,041,060
Application Number:
11/635,355
Assignee:
Synthetic Genomics, Inc. (La Jolla, CA)
DOE Contract Number:  
FG02-02ER63453
Resource Type:
Patent
Resource Relation:
Patent File Date: 2006-12-06
Country of Publication:
United States
Language:
English

Citation Formats

Venter, J. Craig, Smith, Hamilton O., Hutchison, III, Clyde A., and Gibson, Daniel G. Method of nucleic acid cassette assembly. United States: N. p., 2018. Web.
Venter, J. Craig, Smith, Hamilton O., Hutchison, III, Clyde A., & Gibson, Daniel G. Method of nucleic acid cassette assembly. United States.
Venter, J. Craig, Smith, Hamilton O., Hutchison, III, Clyde A., and Gibson, Daniel G. Tue . "Method of nucleic acid cassette assembly". United States. https://www.osti.gov/servlets/purl/1531354.
@article{osti_1531354,
title = {Method of nucleic acid cassette assembly},
author = {Venter, J. Craig and Smith, Hamilton O. and Hutchison, III, Clyde A. and Gibson, Daniel G.},
abstractNote = {Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Rational methods may be used to design the synthetic genome (e.g., to establish a minimal genome and/or to optimize the function of genes within a genome, such as by mutating or rearranging the order of the genes). Synthetic genomes of the invention may be introduced into vesicles (e.g., bacterial cells from which part or all of the resident genome has been removed, or synthetic vesicles) to generate synthetic cells. Synthetic genomes or synthetic cells may be used for a variety of purposes, including the generation of synthetic fuels, such as hydrogen or ethanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {8}
}

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Works referenced in this record:

End-complementary polymerase reaction
patent, July 1999


Method for the mapping of the local AT/GC ratio along DNA
patent, March 2017


DNA polymerase mediated synthesis of double stranded nucleic acids
patent, May 1998


Recombination assembly of large DNA fragments
patent, September 2007


Method for multifragment in vivo cloning and mutation mapping
patent, November 1999


In vitro recombination method
patent, May 2010