skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Electrochemical flow-cell for hydrogen production and nicotinamide dependent target reduction, and related methods and systems

Abstract

Methods and systems for hydrogen production or production of a reduced target molecule are described, wherein a nicotinamide co-factor dependent membrane hydrogenase or a nicotinamide co-factor dependent membrane enzyme presented on a nanolipoprotein adsorbed onto an electrically conductive supporting structure, which can preferably be chemically inert, is contacted with protons or a target molecule to be reduced and nicotinamide cofactors in presence of an electric current and one or more electrically driven redox mediators. Methods and systems for production of hydrogen or a reduced target molecule are also described wherein a membrane-bound hydrogenase enzyme or enzyme capable or reducing a target molecule is contacted with protons or the target molecule, a nicotinamide co-factor and a nicotinamide co-factor dependent membrane hydrogenase presented on a nanolipoprotein particle for a time and under condition to allow hydrogen production or production of a reduced target molecule in presence of an electrical current and of an electrically driven redox mediator.

Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1497681
Patent Number(s):
10,151,037
Application Number:
14/861,750
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Sep 22
Country of Publication:
United States
Language:
English

Citation Formats

. Electrochemical flow-cell for hydrogen production and nicotinamide dependent target reduction, and related methods and systems. United States: N. p., 2018. Web.
. Electrochemical flow-cell for hydrogen production and nicotinamide dependent target reduction, and related methods and systems. United States.
. Tue . "Electrochemical flow-cell for hydrogen production and nicotinamide dependent target reduction, and related methods and systems". United States. https://www.osti.gov/servlets/purl/1497681.
@article{osti_1497681,
title = {Electrochemical flow-cell for hydrogen production and nicotinamide dependent target reduction, and related methods and systems},
author = {},
abstractNote = {Methods and systems for hydrogen production or production of a reduced target molecule are described, wherein a nicotinamide co-factor dependent membrane hydrogenase or a nicotinamide co-factor dependent membrane enzyme presented on a nanolipoprotein adsorbed onto an electrically conductive supporting structure, which can preferably be chemically inert, is contacted with protons or a target molecule to be reduced and nicotinamide cofactors in presence of an electric current and one or more electrically driven redox mediators. Methods and systems for production of hydrogen or a reduced target molecule are also described wherein a membrane-bound hydrogenase enzyme or enzyme capable or reducing a target molecule is contacted with protons or the target molecule, a nicotinamide co-factor and a nicotinamide co-factor dependent membrane hydrogenase presented on a nanolipoprotein particle for a time and under condition to allow hydrogen production or production of a reduced target molecule in presence of an electrical current and of an electrically driven redox mediator.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {12}
}

Patent:

Save / Share:

Works referenced in this record:

Indirect electrochemical reduction of nicotinamide coenzymes
journal, December 2004


Atomic Force Microscope Studies of the Fusion of Floating Lipid Bilayers
journal, June 2007


The Isotope Array, a New Tool That Employs Substrate-Mediated Labeling of rRNA for Determination of Microbial Community Structure and Function
journal, November 2003


Stable isotope probing: Technical considerations when resolving 15N-labeled RNA in gradients
journal, January 2010

  • Addison, S. L.; McDonald, I. R.; Lloyd-Jones, G.
  • Journal of Microbiological Methods, Vol. 80, Issue 1, p. 70-75
  • DOI: 10.1016/j.mimet.2009.11.002

Predictable difficulty or difficulty to predict: Letter to the Editor
journal, December 2010

  • Arányi, Tamás; Fülöp, Krisztina; Symmons, Orsolya
  • Protein Science, Vol. 20, Issue 1
  • DOI: 10.1002/pro.552

Homotropic cooperativity of monomeric cytochrome P450 3A4 in a nanoscale native bilayer environment
journal, October 2004

  • Baas, Bradley J.; Denisov, Ilia G.; Sligar, Stephen G.
  • Archives of Biochemistry and Biophysics, Vol. 430, Issue 2, p. 218-228
  • DOI: 10.1016/j.abb.2004.07.003

Charge‐reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses
journal, September 2001

  • Bacher, Gerold; Szymanski, Wladyslaw W.; Kaufman, Stanley L.
  • Journal of Mass Spectrometry, Vol. 36, Issue 9, p. 1038-1052
  • DOI: 10.1002/jms.208

Hydrogen Production by a Hyperthermophilic Membrane-Bound Hydrogenase in Water-Soluble Nanolipoprotein Particles
journal, June 2009

  • Baker, Sarah E.; Hopkins, Robert C.; Blanchette, Craig D.
  • Journal of the American Chemical Society, Vol. 131, Issue 22
  • DOI: 10.1021/ja809251f

High-Sensitivity Detection of DNA Hybridization on Microarrays Using Resonance Light Scattering
journal, April 2002

  • Bao, Paul; Frutos, Anthony G.; Greef, Charles
  • Analytical Chemistry, Vol. 74, Issue 8
  • DOI: 10.1021/ac0111964

Modulation of human erg K+ channel gating by activation of a G protein-coupled receptor and protein kinase C
journal, September 1998


Reconstitution and Imaging of a Membrane Protein in a Nanometer-Size Phospholipid Bilayer
journal, September 1998

  • Bayburt, Timothy H.; Carlson, Joseph W.; Sligar, Stephen G.
  • Journal of Structural Biology, Vol. 123, Issue 1
  • DOI: 10.1006/jsbi.1998.4007

Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers
journal, November 2003

  • Bayburt, Timothy H.; Sligar, Stephen G.
  • Protein Science, Vol. 12, Issue 11, p. 2476-2481
  • DOI: 10.1110/ps.03267503

Assembly of single bacteriorhodopsin trimers in bilayer nanodiscs
journal, June 2006

  • Bayburt, Timothy H.; Grinkova, Yelena V.; Sligar, Stephen G.
  • Archives of Biochemistry and Biophysics, Vol. 450, Issue 2, p. 215-222
  • DOI: 10.1016/j.abb.2006.03.013

Single-molecule height measurements on microsomal cytochrome P450 in nanometer-scale phospholipid bilayer disks
journal, May 2002

  • Bayburt, T. H.; Sligar, S. G.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 10, p. 6725-6730
  • DOI: 10.1073/pnas.062565599

Self-Assembly of Discoidal Phospholipid Bilayer Nanoparticles with Membrane Scaffold Proteins
journal, August 2002

  • Bayburt, Timothy H.; Grinkova, Yelena V.; Sligar, Stephen G.
  • Nano Letters, Vol. 2, Issue 8, p. 853-856
  • DOI: 10.1021/nl025623k

Transducin Activation by Nanoscale Lipid Bilayers Containing One and Two Rhodopsins
journal, March 2007

  • Bayburt, Timothy H.; Leitz, Andrew J.; Xie, Guifu
  • Journal of Biological Chemistry, Vol. 282, Issue 20
  • DOI: 10.1074/jbc.M701433200

Bacterial Rhodopsin: Evidence for a New Type of Phototrophy in the Sea
journal, September 2000


Specific targeting of a lipophilic prodrug of iododeoxyuridine to parenchymal liver cells using lactosylated reconstituted high density lipoprotein particles
journal, July 1996

  • Bijsterbosch, Martin K.; van de Bilt, Hendrika; van Berkel, Theo J. C.
  • Biochemical Pharmacology, Vol. 52, Issue 1, p. 113-121
  • DOI: 10.1016/0006-2952(96)00170-0

Specific Interaction and Two-Dimensional Crystallization of Histidine Tagged Yeast RNA Polymerase I on Nickel-Chelating Lipids
journal, March 1998

  • Bischler, Nicolas; Balavoine, Fabrice; Milkereit, Philipp
  • Biophysical Journal, Vol. 74, Issue 3, p. 1522-1532
  • DOI: 10.1016/S0006-3495(98)77864-6

Quantifying size distributions of nanolipoprotein particles with single-particle analysis and molecular dynamic simulations
journal, April 2008

  • Blanchette, Craig D.; Law, Richard; Benner, W. Henry
  • Journal of Lipid Research, Vol. 49, Issue 7
  • DOI: 10.1194/jlr.M700586-JLR200

Atomic force microscopy differentiates discrete size distributions between membrane protein containing and empty nanolipoprotein particles
journal, March 2009

  • Blanchette, Craig D.; Cappuccio, Jenny A.; Kuhn, Edward A.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1788, Issue 3, p. 724-731
  • DOI: 10.1016/j.bbamem.2008.11.019

Characterization and Purification of Polydisperse Reconstituted Lipoproteins and Nanolipoprotein Particles
journal, July 2009

  • Blanchette, Craig; Segelke, Brent; Fischer, Nicholas
  • International Journal of Molecular Sciences, Vol. 10, Issue 7
  • DOI: 10.3390/ijms10072958

Nanodiscs separate chemoreceptor oligomeric states and reveal their signaling properties
journal, July 2006

  • Boldog, T.; Grimme, S.; Li, M.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 31, p. 11509-11514
  • DOI: 10.1073/pnas.0604988103

Nanodiscs for Immobilization of Lipid Bilayers and Membrane Receptors: Kinetic Analysis of Cholera Toxin Binding to a Glycolipid Receptor
journal, August 2008

  • Borch, Jonas; Torta, Federico; Sligar, Stephen G.
  • Analytical Chemistry, Vol. 80, Issue 16, p. 6245-6252
  • DOI: 10.1021/ac8000644

Osmotic shrinkage of giant egg-lecithin vesicles
journal, April 1981


Direct linking of microbial populations to specific biogeochemical processes by 13C-labelling of biomarkers
journal, April 1998

  • Boschker, H. T. S.; Nold, S. C.; Wellsbury, P.
  • Nature, Vol. 392, Issue 6678
  • DOI: 10.1038/33900

The contribution of macrophyte-derived organic matter to microbial biomass in salt-marsh sediments: Stable carbon isotope analysis of microbial biomarkers
journal, March 1999

  • Boschker, H. T. S.; de Brouwer, J. F. C.; Cappenberg, T. E.
  • Limnology and Oceanography, Vol. 44, Issue 2
  • DOI: 10.4319/lo.1999.44.2.0309

Formation of Thiolate and Phosphonate Adlayers on Indium−Tin Oxide:  Optical and Electronic Characterization
journal, September 2002

  • Brewer, Scott H.; Brown, Derek A.; Franzen, Stefan
  • Langmuir, Vol. 18, Issue 18
  • DOI: 10.1021/la015720d

Application of a High-Density Oligonucleotide Microarray Approach To Study Bacterial Population Dynamics during Uranium Reduction and Reoxidation
journal, September 2006

  • Brodie, E. L.; DeSantis, T. Z.; Joyner, D. C.
  • Applied and Environmental Microbiology, Vol. 72, Issue 9
  • DOI: 10.1128/AEM.00246-06

Urban aerosols harbor diverse and dynamic bacterial populations
journal, December 2006

  • Brodie, E. L.; DeSantis, T. Z.; Parker, J. P. M.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 1, p. 299-304
  • DOI: 10.1073/pnas.0608255104

Exploring the new world of the genome with DNA microarrays
journal, January 1999

  • Brown, Patrick O.; Botstein, David
  • Nature Genetics, Vol. 21, Issue S1
  • DOI: 10.1038/4462

The Soluble NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha H16 Consists of Six Subunits and Can Be Specifically Activated by NADPH
journal, April 2005


Chemoselective Attachment of Biologically Active Proteins to Surfaces by Expressed Protein Ligation and Its Application for “Protein Chip” Fabrication
journal, November 2004

  • Camarero, Julio A.; Kwon, Youngeun; Coleman, Matthew A.
  • Journal of the American Chemical Society, Vol. 126, Issue 45, p. 14730-14731
  • DOI: 10.1021/ja0456611

Cell-free Co-expression of Functional Membrane Proteins and Apolipoprotein, Forming Soluble Nanolipoprotein Particles
journal, July 2008

  • Cappuccio, Jenny A.; Blanchette, Craig D.; Sulchek, Todd A.
  • Molecular & Cellular Proteomics, Vol. 7, Issue 11
  • DOI: 10.1074/mcp.M800191-MCP200

Protein Prenyltransferases
journal, March 1996

  • Casey, Patrick J.; Seabra, Miguel C.
  • Journal of Biological Chemistry, Vol. 271, Issue 10
  • DOI: 10.1074/jbc.271.10.5289

CpG oligodeoxynucleotides as DNA adjuvants in vertebrates and their applications in immunotherapy
journal, October 2006


Amino acids in SRS1 and SRS6 are critical for furanocoumarin metabolism by CYP6B1v1, a cytochrome P450 monooxygenase
journal, April 2002


Attaching histidine-tagged peptides and proteins to lipid-based carriers through use of metal-ion-chelating lipids
journal, December 2002

  • Chikh, Ghania G.; Li, Wai Ming; Schutze-Redelmeier, Marie-Paule
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1567, p. 204-212
  • DOI: 10.1016/S0005-2736(02)00618-1

Stability of pressure-extruded liposomes made from archaeobacterial ether lipids
journal, November 1994

  • Choquet, C. G.; Patel, G. B.; Sprott, G. D.
  • Applied Microbiology and Biotechnology, Vol. 42, Issue 2-3, p. 375-384
  • DOI: 10.1007/BF00902745

Different Apolipoproteins Impact Nanolipoprotein Particle Formation
journal, November 2007

  • Chromy, Brett A.; Arroyo, Erin; Blanchette, Craig D.
  • Journal of the American Chemical Society, Vol. 129, Issue 46, p. 14348-14354
  • DOI: 10.1021/ja074753y

Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bilayers
journal, September 2003

  • Civjan, Natanya R.; Bayburt, Timothy H.; Schuler, Mary A.
  • BioTechniques, Vol. 35, Issue 3
  • DOI: 10.2144/03353rr02

Integration of biological networks and gene expression data using Cytoscape
journal, September 2007

  • Cline, Melissa S.; Smoot, Michael; Cerami, Ethan
  • Nature Protocols, Vol. 2, Issue 10
  • DOI: 10.1038/nprot.2007.324

Asp 46 can substitute for Asp 96 as the Schiff base proton donor in bacteriorhodopsin
journal, November 1995

  • Coleman, Matthew; Nilsson, Anders; Russell, Terence
  • Biochemistry, Vol. 34, Issue 47
  • DOI: 10.1021/bi00047a027

Kinetic properties of recombinant MAO-A on incorporation into phospholipid nanodisks
journal, March 2007


Hydrogen production by biological processes: a survey of literature
journal, January 2001


Synthesis of Native Proteins by Chemical Ligation
journal, June 2000


Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
journal, July 2006

  • DeSantis, T. Z.; Hugenholtz, P.; Larsen, N.
  • Applied and Environmental Microbiology, Vol. 72, Issue 7, p. 5069-5072
  • DOI: 10.1128/AEM.03006-05

High-Density Universal 16S rRNA Microarray Analysis Reveals Broader Diversity than Typical Clone Library When Sampling the Environment
journal, March 2007


Surface changes induced by osmotic shrinkage on large unilamellar vesicles
journal, November 1996


Rubber Elongation Factor (REF), a Major Allergen Component in Hevea brasiliensis Latex Has Amyloid Properties
journal, October 2012


An improved synthesis of isopentenyl pyrophosphate
journal, November 1967

  • Donninger, C.; Popják, G.
  • Biochemical Journal, Vol. 105, Issue 2
  • DOI: 10.1042/bj1050545

Co-incorporation of heterologously expressed Arabidopsis cytochrome P450 and P450 reductase into soluble nanoscale lipid bilayers
journal, April 2004

  • Duan, Hui; Civjan, Natanya R; Sligar, Stephen G
  • Archives of Biochemistry and Biophysics, Vol. 424, Issue 2, p. 141-153
  • DOI: 10.1016/j.abb.2004.02.010

Dopamine tone regulates D1 receptor trafficking and delivery in striatal neurons in dopamine transporter-deficient mice
journal, February 2000

  • Dumartin, B.; Jaber, M.; Gonon, F.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 4, p. 1879-1884
  • DOI: 10.1073/pnas.97.4.1879

Immobilization of Active Hydrogenases by Encapsulation in Polymeric Porous Gels
journal, October 2005

  • Elgren, Timothy E.; Zadvorny, Oleg A.; Brecht, Eric
  • Nano Letters, Vol. 5, Issue 10, p. 2085-2087
  • DOI: 10.1021/nl0512224

Immobilization of His-Tagged Proteins on Nickel-Chelating Nanolipoprotein Particles
journal, March 2009

  • Fischer, Nicholas O.; Blanchette, Craig D.; Chromy, Brett A.
  • Bioconjugate Chemistry, Vol. 20, Issue 3
  • DOI: 10.1021/bc8003155

IMMUNOLOGY: The Shape of Things to Come
journal, June 2007


Carboxyl-Terminal Domain of Human Apolipoprotein E: Expression, Purification, and Crystallization
journal, November 1999

  • Forstner, Michael; Peters-Libeu, Clare; Contreras-Forrest, Emeline
  • Protein Expression and Purification, Vol. 17, Issue 2
  • DOI: 10.1006/prep.1999.1144

Electron microscopic study on reassembly of plasma high density apoprotein with various lipids
journal, November 1971

  • Forte, T. M.; Nichols, A. V.; Gong, E. L.
  • Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, Vol. 248, Issue 2, p. 381-386
  • DOI: 10.1016/0005-2760(71)90026-9

Role of NADPH-Cytochrome P450 Reductase and Cytochrome- b 5 /NADH- b 5 Reductase in Variability of CYP3A Activity in Human Liver Microsomes
journal, October 2008

  • Gan, Lu; von Moltke, Lisa L.; Trepanier, Lauren A.
  • Drug Metabolism and Disposition, Vol. 37, Issue 1
  • DOI: 10.1124/dmd.108.023424

Molecular cloning of a gene encoding the histamine H2 receptor.
journal, January 1991

  • Gantz, I.; Schaffer, M.; DelValle, J.
  • Proceedings of the National Academy of Sciences, Vol. 88, Issue 2
  • DOI: 10.1073/pnas.88.2.429

Systems for orthogonal self-assembly of electroactive monolayers on Au and ITO: an approach to molecular electronics
journal, July 1995

  • Gardner, Timothy J.; Frisbie, C. Daniel; Wrighton, Mark S.
  • Journal of the American Chemical Society, Vol. 117, Issue 26, p. 6927-6933
  • DOI: 10.1021/ja00131a015

Hydrogen Production under Aerobic Conditions by Membrane-Bound Hydrogenases from Ralstonia Species
journal, August 2008

  • Goldet, Gabrielle; Wait, Annemarie F.; Cracknell, James A.
  • Journal of the American Chemical Society, Vol. 130, Issue 33
  • DOI: 10.1021/ja8027668

Some Observations on the Type I and Type II Microsomal Binding Spectra
journal, January 1971


Protein tolerance to random amino acid change
journal, June 2004

  • Guo, H. H.; Choe, J.; Loeb, L. A.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210
  • DOI: 10.1073/pnas.0403255101

Adjuvants for human vaccines—current status, problems and future prospects
journal, January 1995


Complex of Human Apolipoprotein C-1 with Phospholipid:  Thermodynamic or Kinetic Stability?
journal, June 2002

  • Gursky, Olga; Gantz, Donald L.
  • Biochemistry, Vol. 41, Issue 23
  • DOI: 10.1021/bi025588w

Biological hydrogen production; fundamentals and limiting processes
journal, November 2002


Pharmaceutical analysis of synthetic lipid A-based vaccine adjuvants in poly (d,l-lactic-co-glycolic acid) nanoparticle formulations
journal, August 2007

  • Hamdy, Samar; Haddadi, Azita; Somayaji, Vishwa
  • Journal of Pharmaceutical and Biomedical Analysis, Vol. 44, Issue 4, p. 914-923
  • DOI: 10.1016/j.jpba.2007.03.010

Structure and function of cytochromes P450:a comparative analysis of three crystal structures
journal, January 1995


Energy-Converting [NiFe] Hydrogenases from Archaea and Extremophiles: Ancestors of Complex I
journal, February 2004


Influence of liposome charge and composition on their interaction with human blood serum proteins
journal, January 1993

  • Hernández-Caselles, Trinidad; Villalaín, José; Gómez-Fernández, Juan C.
  • Molecular and Cellular Biochemistry, Vol. 120, Issue 2, p. 119-126
  • DOI: 10.1007/BF00926084

Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia coli
journal, March 1998

  • Hill, Margaret A.; Preiss, Jack
  • Biochemical and Biophysical Research Communications, Vol. 244, Issue 2, p. 573-577
  • DOI: 10.1006/bbrc.1998.8301

Enzyme-catalyzed Synthesis and Degradation of Biopolymers
journal, February 2009


Fabrication of biomolecule–copolymer hybrid nanovesicles as energy conversion systems
journal, November 2005


G-Protein-Coupled Receptor Microarrays for Multiplexed Compound Screening
journal, June 2006

  • Hong, Yulong; Webb, Brian L.; Pai, Sadashiva
  • Journal of Biomolecular Screening, Vol. 11, Issue 4
  • DOI: 10.1177/1087057106287139

Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors
journal, May 2005

  • Ishihara, Goshi; Goto, Mie; Saeki, Mihoro
  • Protein Expression and Purification, Vol. 41, Issue 1, p. 27-37
  • DOI: 10.1016/j.pep.2005.01.013

Indium-111 Labeling of Low Density Lipoproteins with the DTPA−Bis(stearylamide):  Evaluation as a Potential Radiopharmaceutical for Tumor Localization
journal, January 1996

  • Jasanada, François; Urizzi, Pascale; Souchard, Jean-Pierre
  • Bioconjugate Chemistry, Vol. 7, Issue 1
  • DOI: 10.1021/bc950073l

Structural Basis for Thermal Stability of Human Low-Density Lipoprotein
journal, March 2005

  • Jayaraman, Shobini; Gantz, Donald; Gursky, Olga
  • Biochemistry, Vol. 44, Issue 10, p. 3965-3971
  • DOI: 10.1021/bi047493v

Functional Cell-free Synthesis of a Seven Helix Membrane Protein: In situ Insertion of Bacteriorhodopsin into Liposomes
journal, August 2007

  • Kalmbach, Rolf; Chizhov, Igor; Schumacher, Miria C.
  • Journal of Molecular Biology, Vol. 371, Issue 3, p. 639-648
  • DOI: 10.1016/j.jmb.2007.05.087

Bio-hydrogen production from waste materials
journal, March 2006


Insertion of Membrane Proteins into Discoidal Membranes Using a Cell-Free Protein Expression Approach
journal, August 2008

  • Katzen, Federico; Fletcher, Julia E.; Yang, Jian-Ping
  • Journal of Proteome Research, Vol. 7, Issue 8
  • DOI: 10.1021/pr800265f

Gold Nanoparticle-Enhanced Secondary Ion Mass Spectrometry Imaging of Peptides on Self-Assembled Monolayers
journal, March 2006

  • Kim, Young-Pil; Oh, Eunkeu; Hong, Mi-Young
  • Analytical Chemistry, Vol. 78, Issue 6
  • DOI: 10.1021/ac051500j

High level cell-free expression and specific labeling of integral membrane proteins
journal, February 2004


Cell-free expression as an emerging technique for the large scale production of integral membrane protein
journal, September 2006


The growing impact of click chemistry on drug discovery
journal, December 2003


Chemical modification of hemoglobins: a study of conformation restraint by internal bridging.
journal, August 1966

  • Simon, S. R.; Konigsberg, W. H.
  • Proceedings of the National Academy of Sciences, Vol. 56, Issue 2
  • DOI: 10.1073/pnas.56.2.749

A novel approach for biohydrogen production
journal, September 2006


Two-Dimensional Crystallization of Histidine-Tagged, HIV-1 Reverse Transcriptase Promoted by a Novel Nickel-Chelating Lipid
journal, September 1994

  • Kubalek, Elizabeth W.; Le Grice, Stuart F. J.; Brown, Patrick O.
  • Journal of Structural Biology, Vol. 113, Issue 2
  • DOI: 10.1006/jsbi.1994.1039

Novel applications of liposomes
journal, July 1998


Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities.
journal, March 1988

  • Lazar, E.; Watanabe, S.; Dalton, S.
  • Molecular and Cellular Biology, Vol. 8, Issue 3, p. 1247-1252
  • DOI: 10.1128/MCB.8.3.1247

Functional reconstitution of β 2 -adrenergic receptors utilizing self-assembling Nanodisc technology
journal, May 2006

  • Leitz, Andrew J.; Bayburt, Timothy H.; Barnakov, Alexander N.
  • BioTechniques, Vol. 40, Issue 5
  • DOI: 10.2144/000112169

Mechanical properties and stability measurement of cholesterol-containing liposome on mica by atomic force microscopy
journal, October 2004

  • Liang, Xuemei; Mao, Guangzhao; Ng, K. Y. Simon
  • Journal of Colloid and Interface Science, Vol. 278, Issue 1, p. 53-62
  • DOI: 10.1016/j.jcis.2004.05.042

Characterization of a HoxEFUYH type of [NiFe] hydrogenase from Allochromatium vinosum and some EPR and IR properties of the hydrogenase module
journal, September 2006

  • Long, Minnan; Liu, Jingjing; Chen, Zhifeng
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 12, Issue 1
  • DOI: 10.1007/s00775-006-0162-1

Conformational Reorganization of the Four-helix Bundle of Human Apolipoprotein E in Binding to Phospholipid
journal, May 2000

  • Lu, Bin; Morrow, Julie A.; Weisgraber, Karl H.
  • Journal of Biological Chemistry, Vol. 275, Issue 27, p. 20775-20781
  • DOI: 10.1074/jbc.M003508200

ARB: a software environment for sequence data
journal, February 2004


RNA Stable Isotope Probing, a Novel Means of Linking Microbial Community Function to Phylogeny
journal, November 2002


Large-scale profiling of protein palmitoylation in mammalian cells
journal, January 2009

  • Martin, Brent R.; Cravatt, Benjamin F.
  • Nature Methods, Vol. 6, Issue 2, p. 135-138
  • DOI: 10.1038/nmeth.1293

The Vaccine Adjuvant Monophosphoryl Lipid A as a TRIF-Biased Agonist of TLR4
journal, June 2007


The Efficiency of Light-Directed Synthesis of DNA Arrays on Glass Substrates
journal, June 1997

  • McGall, Glenn H.; Barone, Anthony D.; Diggelmann, Martin
  • Journal of the American Chemical Society, Vol. 119, Issue 22, p. 5081-5090
  • DOI: 10.1021/ja964427a

The [NiFe]-Hydrogenase of the Cyanobacterium Synechocystis sp. PCC 6803 Works Bidirectionally with a Bias to H 2 Production
journal, July 2011

  • McIntosh, Chelsea L.; Germer, Frauke; Schulz, Rüdiger
  • Journal of the American Chemical Society, Vol. 133, Issue 29
  • DOI: 10.1021/ja203376y

Intact Functional Fourteen-subunit Respiratory Membrane-bound [NiFe]-Hydrogenase Complex of the Hyperthermophilic Archaeon Pyrococcus furiosus
journal, May 2014

  • McTernan, Patrick M.; Chandrayan, Sanjeev K.; Wu, Chang-Hao
  • Journal of Biological Chemistry, Vol. 289, Issue 28
  • DOI: 10.1074/jbc.M114.567255

Evaluation of Nanolipoprotein Particles (NLPs) as an In Vivo Delivery Platform
journal, March 2014


Protein engineering methods applied to membrane protein targets
journal, October 2012

  • Lluis, M. W.; Godfroy, J. I.; Yin, H.
  • Protein Engineering Design and Selection, Vol. 26, Issue 2
  • DOI: 10.1093/protein/gzs079

ACTH, α-MSH, and control of cortisol release: cloning, sequencing, and functional expression of the melanocortin-2 and melanocortin-5 receptor in C yprinus carpio
journal, September 2005

  • Metz, Juriaan R.; Geven, Edwin J. W.; van den Burg, Erwin H.
  • American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Vol. 289, Issue 3
  • DOI: 10.1152/ajpregu.00826.2004

Purification and catalytic properties of Ech hydrogenase from Methanosarcina barkeri
journal, October 1999


[FeFe] hydrogenases and their evolution: a genomic perspective
journal, March 2007


Functional Characterization of Apolipoprotein E Isoforms Overexpressed in Escherichia coli
journal, July 1999

  • Morrow, Julie A.; Arnold, Kay S.; Weisgraber, Karl H.
  • Protein Expression and Purification, Vol. 16, Issue 2
  • DOI: 10.1006/prep.1999.1069

Detection of DNA hybridization on indium tin oxide surfaces
journal, August 2007

  • Moses, Selina; Brewer, Scott H.; Kraemer, Stephan
  • Sensors and Actuators B: Chemical, Vol. 125, Issue 2, p. 574-580
  • DOI: 10.1016/j.snb.2007.03.009

Applications of Phospholipid Bilayer Nanodiscs in the Study of Membranes and Membrane Proteins
journal, February 2007

  • Nath, Abhinav; Atkins, William M.; Sligar, Stephen G.
  • Biochemistry, Vol. 46, Issue 8
  • DOI: 10.1021/bi602371n

A Potent Adjuvant Monophosphoryl Lipid A Triggers Various Immune Responses, but Not Secretion of IL-1β or Activation of Caspase-1
journal, January 2006


The Difference a Se Makes? Oxygen-Tolerant Hydrogen Production by the [NiFeSe]-Hydrogenase from Desulfomicrobium baculatum
journal, October 2008

  • Parkin, Alison; Goldet, Gabrielle; Cavazza, Christine
  • Journal of the American Chemical Society, Vol. 130, Issue 40
  • DOI: 10.1021/ja803657d

In-depth analysis of the membrane and cytosolic proteome of red blood cells
journal, August 2006


Preparation and Characterization of Nickel Nanoparticles for Binding to His-tag Proteins and Antigens
journal, December 2006


The Biosynthesis of Natural Rubber
journal, June 1990

  • Paterson-Jones, J. C.; Gilliland, M. G.; Van Staden, J.
  • Journal of Plant Physiology, Vol. 136, Issue 3, p. 257-263
  • DOI: 10.1016/S0176-1617(11)80047-7

Taking toll: lipid A mimetics as adjuvants and immunomodulators
journal, October 2002


Model of Biologically Active Apolipoprotein E Bound to Dipalmitoylphosphatidylcholine
journal, November 2005

  • Peters-Libeu, Clare A.; Newhouse, Yvonne; Hatters, Danny M.
  • Journal of Biological Chemistry, Vol. 281, Issue 2
  • DOI: 10.1074/jbc.M510851200

The Effects of Deleting the Mouse Neurotensin Receptor NTR1 on Central and Peripheral Responses to Neurotensin
journal, January 2002

  • Pettibone, Douglas J.; Hess, J. Fred; Hey, Patricia J.
  • Journal of Pharmacology and Experimental Therapeutics, Vol. 300, Issue 1
  • DOI: 10.1124/jpet.300.1.305

Transcriptome and gene expression analysis in cold-acclimated guayule (Parthenium argentatum) rubber-producing tissue
journal, July 2012


Identification of active methylotroph populations in an acidic forest soil by stable-isotope probing c
journal, August 2002


Stable-isotope probing as a tool in microbial ecology
journal, February 2000

  • Radajewski, Stefan; Ineson, Philip; Parekh, Nisha R.
  • Nature, Vol. 403, Issue 6770, p. 646-649
  • DOI: 10.1038/35001054

Cyanobacterial-Type, Heteropentameric, NAD+-Reducing NiFe Hydrogenase in the Purple Sulfur Photosynthetic Bacterium Thiocapsa roseopersicina
journal, February 2004


Comparison of Multiplexed Techniques for Detection of Bacterial and Viral Proteins
journal, August 2004

  • Rao, Rupa S.; Visuri, Steven R.; McBride, Mary T.
  • Journal of Proteome Research, Vol. 3, Issue 4, p. 736-742
  • DOI: 10.1021/pr034130t

Two-Dimensional Streptavidin Crystals on Giant Lipid Bilayer Vesicles
journal, May 2002

  • Ratanabanangkoon, Pasut; Gropper, Michael; Merkel, Rudolf
  • Langmuir, Vol. 18, Issue 11, p. 4270-4276
  • DOI: 10.1021/la025568v

Different G protein-coupled receptor kinases govern G protein and β-arrestin-mediated signaling of V2 vasopressin receptor
journal, January 2005

  • Ren, X.-R.; Reiter, E.; Ahn, S.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 5, p. 1448-1453
  • DOI: 10.1073/pnas.0409534102

Generation of immunoliposomes using recombinant single-chain Fv fragments bound to Ni-NTA-liposomes
journal, August 2005


In vitro characterization of binding and stability of single-chain Fv Ni-NTA-liposomes
journal, January 2006


In Vitro Reconstitution of Assembly of Apolipoprotein B48-containing Lipoproteins
journal, March 1997

  • Rusiñol, Antonio E.; Jamil, Haris; Vance, Jean E.
  • Journal of Biological Chemistry, Vol. 272, Issue 12
  • DOI: 10.1074/jbc.272.12.8019

Chemistry: The photon trap
journal, March 2008


A simple energy-conserving system: Proton reduction coupled to proton translocation
journal, June 2003

  • Sapra, R.; Bagramyan, K.; Adams, M. W. W.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 13, p. 7545-7550
  • DOI: 10.1073/pnas.1331436100

Purification and Characterization of a Membrane-Bound Hydrogenase from the Hyperthermophilic Archaeon Pyrococcus furiosus
journal, June 2000


A bilayer cell-free protein synthesis system for high-throughput screening of gene products
journal, February 2002


Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
journal, October 1995


Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz
journal, January 2010

  • Schmidt, Thomas; Lenders, Malte; Hillebrand, Andrea
  • BMC Biochemistry, Vol. 11, Issue 1
  • DOI: 10.1186/1471-2091-11-11

Synthesis and Characterization of Chelator-Lipids for Reversible Immobilization of Engineered Proteins at Self-Assembled Lipid Interfaces
journal, September 1994

  • Schmitt, Lutz; Dietrich, Christian; Tampe, Robert
  • Journal of the American Chemical Society, Vol. 116, Issue 19, p. 8485-8491
  • DOI: 10.1021/ja00098a008

HoxE—a subunit specific for the pentameric bidirectional hydrogenase complex (HoxEFUYH) of cyanobacteria
journal, April 2002

  • Schmitz, Oliver; Boison, Gudrun; Salzmann, Heike
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1554, Issue 1-2, p. 66-74
  • DOI: 10.1016/S0005-2728(02)00214-1

Protein Translocons
journal, February 2003


Laboratory scale structural genomics
journal, March 2004

  • Segelke, Brent W.; Schafer, Johana; Coleman, Matthew A.
  • Journal of Structural and Functional Genomics, Vol. 5, Issue 1-2, p. 147-157
  • DOI: 10.1023/B:JSFG.0000029193.82120.d1

Phospholipid phase transitions in homogeneous nanometer scale bilayer discs
journal, December 2003


Molecular Dynamics Simulations of Discoidal Bilayers Assembled from Truncated Human Lipoproteins
journal, January 2005


The micromorphology and protein characterization of rubber particles in Ficus carica, Ficus benghalensis and Hevea brasiliensis
journal, March 2003

  • Singh, A. P.; Wi, S. G.; Chung, G. C.
  • Journal of Experimental Botany, Vol. 54, Issue 384
  • DOI: 10.1093/jxb/erg107

Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array
journal, October 1999

  • Singh-Gasson, Sangeet; Green, Roland D.; Yue, Yongjian
  • Nature Biotechnology, Vol. 17, Issue 10, p. 974-978
  • DOI: 10.1038/13664

Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans: CO-oxidizing:H2-evolving enzyme complex
journal, October 2002


Cell-free synthesis, functional refolding, and spectroscopic characterization of bacteriorhodopsin, an integral membrane protein
journal, December 1993

  • Sonar, Sanjay; Patel, Nilam; Fischer, Wolfgang
  • Biochemistry, Vol. 32, Issue 50
  • DOI: 10.1021/bi00213a004

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles
journal, February 2010

  • Sperling, R. A.; Parak, W. J.
  • Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 368, Issue 1915, p. 1333-1383
  • DOI: 10.1098/rsta.2009.0273

Membrane-Mimetic Films of Asymmetric Phosphatidylcholine Lipid Bolaamphiphiles
journal, January 2006

  • Sun, Xue-Long; Biswas, Nilanjana; Kai, Toshitsugu
  • Langmuir, Vol. 22, Issue 3
  • DOI: 10.1021/la052125t

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems
journal, January 2003


Peptide stabilized amphotericin B nanodisks
journal, April 2007


Topology prediction of membrane proteins
journal, February 1996


Recombinant lipoproteins: lipoprotein-like lipid particles for drug targeting
journal, April 2001

  • Rensen, Patrick C. N.; de Vrueh, Remco L. A.; Kuiper, Johan
  • Advanced Drug Delivery Reviews, Vol. 47, Issue 2-3, p. 251-276
  • DOI: 10.1016/S0169-409X(01)00109-0

Overview of Protein Structural and Functional Folds
book, February 2004

  • Sun, Peter D.; Foster, Christine E.; Boyington, Jeffrey C.
  • Current Protocols in Protein Science, Vol. 35, p. 17.1.1.1–17.1.189
  • DOI: 10.1002/0471140864.ps1701s35

Induction of tumor necrosis factor-α in solid tumor region by the orally administered synthetic muramyl dipeptide analogue, romurtide
journal, January 2001


DNA-based stable isotope probing: a link between community structure and function
journal, June 2009

  • Uhlík, Ondrej; Jecná, Katerina; Leigh, Mary Beth
  • Science of The Total Environment, Vol. 407, Issue 12, p. 3611-3619
  • DOI: 10.1016/j.scitotenv.2008.05.012

Vaccine manufacturing: challenges and solutions
journal, November 2006

  • Ulmer, Jeffrey B.; Valley, Ulrich; Rappuoli, Rino
  • Nature Biotechnology, Vol. 24, Issue 11
  • DOI: 10.1038/nbt1261

Occurrence, Classification, and Biological Function of Hydrogenases:  An Overview
journal, October 2007

  • Vignais, Paulette M.; Billoud, Bernard
  • Chemical Reviews, Vol. 107, Issue 10, p. 4206-4272
  • DOI: 10.1021/cr050196r

Investigating and Exploiting the Electrocatalytic Properties of Hydrogenases
journal, October 2007

  • Vincent, Kylie A.; Parkin, Alison; Armstrong, Fraser A.
  • Chemical Reviews, Vol. 107, Issue 10, p. 4366-4413
  • DOI: 10.1021/cr050191u

Disentangling the perturbational effects of amino acid substitutions in the DNA-binding domain of p53
journal, February 1999

  • Wacey, A. I.; Cooper, D. N.; Liney, D.
  • Human Genetics, Vol. 104, Issue 1, p. 15-22
  • DOI: 10.1007/s004390050904

Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms
journal, April 1998


Comparison of the Dynamics of the Primary Events of Bacteriorhodopsin in Its Trimeric and Monomeric States
journal, September 2002


CpG DNA induces stronger immune responses with less toxicity than other adjuvants
journal, March 2000


How Translocons Select Transmembrane Helices
journal, June 2008


A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein
journal, April 2007

  • Whorton, M. R.; Bokoch, M. P.; Rasmussen, S. G. F.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 18, p. 7682-7687
  • DOI: 10.1073/pnas.0611448104

Construction and characterization of a second-generation pseudoinfectious West Nile virus vaccine propagated using a new cultivation system
journal, May 2008


Enzymatic production of biohydrogen: Biotechnology
journal, June 2000

  • Woodward, Jonathan; Orr, Mark; Cordray, Kimberley
  • Nature, Vol. 405, Issue 6790, p. 1014-1015
  • DOI: 10.1038/35016633

In vitro hydrogen production by glucose dehydrogenase and hydrogenase
journal, July 1996

  • Woodward, Jonathan; Mattingly, Susan M.; Danson, Michael
  • Nature Biotechnology, Vol. 14, Issue 7, p. 872-874
  • DOI: 10.1038/nbt0796-872

High yield cell-free production of integral membrane proteins without refolding or detergents
journal, May 2008

  • Wuu, Jessica J.; Swartz, James R.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1778, Issue 5, p. 1237-1250
  • DOI: 10.1016/j.bbamem.2008.01.023

Initiation of rubber biosynthesis: In vitro comparisons of benzophenone-modified diphosphate analogues in three rubber-producing species
journal, October 2008


High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway
journal, May 2007

  • Zhang, Y.-H. Percival; Evans, Barbara R.; Mielenz, Jonathan R.
  • PLoS ONE, Vol. 2, Issue 5, Article No. e456
  • DOI: 10.1371/journal.pone.0000456

Protein subunit interfaces: heterodimers versus homodimers
journal, August 2005


Immunostimulatory DNA as adjuvant: efficacy of phosphodiester CpG oligonucleotides is enhanced by 3′ sequence modifications
journal, February 2003


From The Cover: Electrocatalytic hydrogen oxidation by an enzyme at high carbon monoxide or oxygen levels
journal, October 2005

  • Vincent, K. A.; Cracknell, J. A.; Lenz, O.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 47, p. 16951-16954
  • DOI: 10.1073/pnas.0504499102

Thermotolerant Hydrogenases: Biological Diversity, Properties, and Biotechnological Applications
journal, January 2008