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Title: Electrochemical flow-cell for hydrogen production and nicotinamide dependent target reduction, and related methods and systems


Methods and systems for hydrogen production or production of a reduced target molecule are described, wherein a nicotinamide co-factor dependent membrane hydrogenase or a nicotinamide co-factor dependent membrane enzyme presented on a nanolipoprotein adsorbed onto an electrically conductive supporting structure, which can preferably be chemically inert, is contacted with protons or a target molecule to be reduced and nicotinamide cofactors in presence of an electric current and one or more electrically driven redox mediators. Methods and systems for production of hydrogen or a reduced target molecule are also described wherein a membrane-bound hydrogenase enzyme or enzyme capable or reducing a target molecule is contacted with protons or the target molecule, a nicotinamide co-factor and a nicotinamide co-factor dependent membrane hydrogenase presented on a nanolipoprotein particle for a time and under condition to allow hydrogen production or production of a reduced target molecule in presence of an electrical current and of an electrically driven redox mediator.

Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
OSTI Identifier:
Patent Number(s):
Application Number:
Lawrence Livermore National Security, LLC (Livermore, CA)
DOE Contract Number:  
Resource Type:
Resource Relation:
Patent File Date: 2015 Sep 22
Country of Publication:
United States

Citation Formats

. Electrochemical flow-cell for hydrogen production and nicotinamide dependent target reduction, and related methods and systems. United States: N. p., 2018. Web.
. Electrochemical flow-cell for hydrogen production and nicotinamide dependent target reduction, and related methods and systems. United States.
. Tue . "Electrochemical flow-cell for hydrogen production and nicotinamide dependent target reduction, and related methods and systems". United States.
title = {Electrochemical flow-cell for hydrogen production and nicotinamide dependent target reduction, and related methods and systems},
author = {},
abstractNote = {Methods and systems for hydrogen production or production of a reduced target molecule are described, wherein a nicotinamide co-factor dependent membrane hydrogenase or a nicotinamide co-factor dependent membrane enzyme presented on a nanolipoprotein adsorbed onto an electrically conductive supporting structure, which can preferably be chemically inert, is contacted with protons or a target molecule to be reduced and nicotinamide cofactors in presence of an electric current and one or more electrically driven redox mediators. Methods and systems for production of hydrogen or a reduced target molecule are also described wherein a membrane-bound hydrogenase enzyme or enzyme capable or reducing a target molecule is contacted with protons or the target molecule, a nicotinamide co-factor and a nicotinamide co-factor dependent membrane hydrogenase presented on a nanolipoprotein particle for a time and under condition to allow hydrogen production or production of a reduced target molecule in presence of an electrical current and of an electrically driven redox mediator.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {12}


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