Gene modification in clostridium for increased alcohol production
Abstract
The present disclosure is directed to genetically engineered bacteria strains with enhanced biofuel-producing capabilities from cellulosic substrates. The bacteria strains of the present disclosure comprise an inactivated Type I glutamine synthetase gene. The present disclosure is also directed to methods of producing biofuels from cellulosic biomass using the genetically engineered bacteria strains.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1496801
- Patent Number(s):
- 10179907
- Application Number:
- 15/643,580
- Assignee:
- UT-BATTELLE, LLC (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC05-000R22725
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2017 Jul 07
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Rydzak, Thomas, and Guss, Adam M. Gene modification in clostridium for increased alcohol production. United States: N. p., 2019.
Web.
Rydzak, Thomas, & Guss, Adam M. Gene modification in clostridium for increased alcohol production. United States.
Rydzak, Thomas, and Guss, Adam M. Tue .
"Gene modification in clostridium for increased alcohol production". United States. https://www.osti.gov/servlets/purl/1496801.
@article{osti_1496801,
title = {Gene modification in clostridium for increased alcohol production},
author = {Rydzak, Thomas and Guss, Adam M.},
abstractNote = {The present disclosure is directed to genetically engineered bacteria strains with enhanced biofuel-producing capabilities from cellulosic substrates. The bacteria strains of the present disclosure comprise an inactivated Type I glutamine synthetase gene. The present disclosure is also directed to methods of producing biofuels from cellulosic biomass using the genetically engineered bacteria strains.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {1}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Elimination of formate production in Clostridium thermocellum
journal, July 2015
- Rydzak, Thomas; Lynd, Lee R.; Guss, Adam M.
- Journal of Industrial Microbiology & Biotechnology, Vol. 42, Issue 9
Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress
journal, January 2013
- Wilson, Charlotte M.; Yang, Shihui; Rodriguez, Miguel
- Biotechnology for Biofuels, Vol. 6, Issue 1
Carbon Flux Distribution and Kinetics of Cellulose Fermentation in Steady-State Continuous Cultures of Clostridium cellulolyticum on a Chemically Defined Medium
journal, January 2001
- Desvaux, M.; Guedon, E.; Petitdemange, H.
- Journal of Bacteriology, Vol. 183, Issue 1
NADP+ Reduction with Reduced Ferredoxin and NADP+ Reduction with NADH Are Coupled via an Electron-Bifurcating Enzyme Complex in Clostridium kluyveri
journal, July 2010
- Wang, S.; Huang, H.; Moll, J.
- Journal of Bacteriology, Vol. 192, Issue 19
Development of pyrF-Based Genetic System for Targeted Gene Deletion in Clostridium thermocellum and Creation of a pta Mutant
journal, August 2010
- Tripathi, S. A.; Olson, D. G.; Argyros, D. A.
- Applied and Environmental Microbiology, Vol. 76, Issue 19, p. 6591-6599
Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways
journal, May 2013
- van der Veen, Douwe; Lo, Jonathan; Brown, Steven D.
- Journal of Industrial Microbiology & Biotechnology, Vol. 40, Issue 7
Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum
journal, January 2015
- Biswas, Ranjita; Zheng, Tianyong; Olson, Daniel G.
- Biotechnology for Biofuels, Vol. 8, Issue 1
Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum
journal, January 2012
- Guss, Adam M.; Olson, Daniel G.; Caiazza, Nicky C.
- Biotechnology for Biofuels, Vol. 5, Issue 1
High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes
journal, September 2011
- Argyros, D. Aaron; Tripathi, Shital A.; Barrett, Trisha F.
- Applied and Environmental Microbiology, Vol. 77, Issue 23, p. 8288-8294
Proteomic analysis of Clostridium thermocellum core metabolism: relative protein expression profiles and growth phase-dependent changes in protein expression
journal, January 2012
- Rydzak, Thomas; McQueen, Peter D.; Krokhin, Oleg V.
- BMC Microbiology, Vol. 12, Issue 1
Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405)
journal, January 2012
- Ellis, Lucas D.; Holwerda, Evert K.; Hogsett, David
- Bioresource Technology, Vol. 103, Issue 1
The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading
journal, October 2014
- Holwerda, Evert K.; Thorne, Philip G.; Olson, Daniel G.
- Biotechnology for Biofuels, Vol. 7, Issue 1
Kinetics and Metabolism of Cellulose Degradation at High Substrate Concentrations in Steady-State Continuous Cultures of Clostridium cellulolyticum on a Chemically Defined Medium
journal, September 2001
- Desvaux, M.; Guedon, E.; Petitdemange, H.
- Applied and Environmental Microbiology, Vol. 67, Issue 9
Insights into electron flux through manipulation of fermentation conditions and assessment of protein expression profiles in Clostridium thermocellum
journal, May 2014
- Rydzak, Thomas; Grigoryan, Marina; Cunningham, Zack J.
- Applied Microbiology and Biotechnology, Vol. 98, Issue 14
Metabolic flux in cellulose batch and cellulose-fed continuous cultures of Clostridium cellulolyticum in response to acidic environment
journal, June 2001
- Petitdemange, Henri; Guedon, Emmanuel; Desvaux, Mickaël
- Microbiology, Vol. 147, Issue 6
Elimination of metabolic pathways to all traditional fermentation products increases ethanol yields in Clostridium thermocellum
journal, November 2015
- Papanek, Beth; Biswas, Ranjita; Rydzak, Thomas
- Metabolic Engineering, Vol. 32
Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum
journal, January 2013
- Deng, Yu; Olson, Daniel G.; Zhou, Jilai
- Metabolic Engineering, Vol. 15, p. 151-158