Title: Gene modification in clostridium for increased alcohol production

Abstract

The present disclosure is directed to genetically engineered bacteria strains with enhanced biofuel-producing capabilities from cellulosic substrates. The bacteria strains of the present disclosure comprise an inactivated Type I glutamine synthetase gene. The present disclosure is also directed to methods of producing biofuels from cellulosic biomass using the genetically engineered bacteria strains.

Inventors:

Rydzak, Thomas; Guss, Adam M.

Issue Date:

2019-01-15

Research Org.:

Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1496801

Patent Number(s):

10179907

Application Number:

15/643,580

Assignee:

UT-BATTELLE, LLC (Oak Ridge, TN)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/93 - {Ligases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/065 - {with microorganisms other than yeasts}
C12P7/10 - substrate containing cellulosic material
C12P7/16 - Butanols

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y603/01002 - Glutamate-ammonia ligase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

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DOE Contract Number:  

AC05-000R22725

Resource Type:

Patent

Resource Relation:

Patent File Date: 2017 Jul 07

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES