Title: Trajectory-based triggering system for hyperspectral imaging flow cytometer

Abstract

A hyperspectral imaging flow cytometer can acquire high-resolution hyperspectral images of particles, such as biological cells, flowing through a microfluidic system. A trajectory-based triggering system can be used that will only trigger the acquisition of a hyperspectral image when an appropriate particle or cell is crossing an imaging line, thereby saving valuable resources and time. The hyperspectral imaging flow cytometer can provide detailed spatial maps of multiple emitting species, cell morphology information, and state of health. An optimized system can image about 20 cells per second. The hyperspectral imaging flow cytometer enables many thousands of cells to be characterized in a single session.

Inventors:

Sinclair, Michael B.

Issue Date:

2019-01-15

Research Org.:

Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1496794

Patent Number(s):

10180398

Application Number:

15/681,824

Assignee:

National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2200/0652 - Sorting or classification of particles or molecules
B01L2200/0673 - Handling of plugs of fluid surrounded by immiscible fluid
B01L2300/0627 - Sensor or part of a sensor is integrated
B01L2400/0424 - Dielectrophoretic forces
B01L3/502761 - {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N15/1427 - {with the synchronisation of components, a time gate for operation of components, or suppression of particle coincidences}
G01N15/1436 - {the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell}
G01N15/1459 - {the analysis being performed on a sample stream}
G01N15/147 - {the analysis being performed on a sample stream}
G01N15/1484 - {microstructural devices}
G01N2015/1006 - {for cytology}
G01N2015/144 - {Imaging characterised by its optical setup}
G01N2015/149 - {Sorting the particles}
G01N2021/6439 - {with indicators, stains, dyes, tags, labels, marks}
G01N21/6428 - {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"

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DOE Contract Number:  

AC04-94AL85000

Resource Type:

Patent

Resource Relation:

Patent File Date: 2017 Aug 21

Country of Publication:

United States

Language:

English

Subject:

47 OTHER INSTRUMENTATION