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Title: Microfluidic platform for synthetic biology applications

Abstract

This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to form a sequenced order of droplets carrying different components of the biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of the biological construct to permit the components to react with each other in one or more reactions contributing to the assembly of the biological construct.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1494577
Patent Number(s):
10131903
Application Number:
13/437,727
Assignee:
The Regents of the University of California (Oakland, CA); Sandia Corporation (Livermore, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Apr 02
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chang, Chieh, Bharadwa, Rajiv, Singh, Anup K., Chandrasekaran, Aarthi, and Hillson, Nathan J.. Microfluidic platform for synthetic biology applications. United States: N. p., 2018. Web.
Chang, Chieh, Bharadwa, Rajiv, Singh, Anup K., Chandrasekaran, Aarthi, & Hillson, Nathan J.. Microfluidic platform for synthetic biology applications. United States.
Chang, Chieh, Bharadwa, Rajiv, Singh, Anup K., Chandrasekaran, Aarthi, and Hillson, Nathan J.. Tue . "Microfluidic platform for synthetic biology applications". United States. https://www.osti.gov/servlets/purl/1494577.
@article{osti_1494577,
title = {Microfluidic platform for synthetic biology applications},
author = {Chang, Chieh and Bharadwa, Rajiv and Singh, Anup K. and Chandrasekaran, Aarthi and Hillson, Nathan J.},
abstractNote = {This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to form a sequenced order of droplets carrying different components of the biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of the biological construct to permit the components to react with each other in one or more reactions contributing to the assembly of the biological construct.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {11}
}

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Works referenced in this record:

A Functional Analysis of the Spacer of V(D)J Recombination Signal Sequences
journal, October 2003


DNA assembly for synthetic biology: from parts to pathways and beyond
journal, January 2011


Molecular biology on a microfluidic chip
journal, April 2006


Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes
journal, May 2009


Microfluidic self-assembly of live Drosophila embryos for versatile high-throughput analysis of embryonic morphogenesis
journal, May 2007


An Integrated Microfluidic System for Reaction, High-Sensitivity Detection, and Sorting of Fluorescent Cells and Particles
journal, November 2003