Microfluidic platform for synthetic biology applications
Abstract
This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to form a sequenced order of droplets carrying different components of the biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of the biological construct to permit the components to react with each other in one or more reactions contributing to the assembly of the biological construct.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1494577
- Patent Number(s):
- 10131903
- Application Number:
- 13/437,727
- Assignee:
- The Regents of the University of California (Oakland, CA); Sandia Corporation (Livermore, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Apr 02
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Chang, Chieh, Bharadwa, Rajiv, Singh, Anup K., Chandrasekaran, Aarthi, and Hillson, Nathan J. Microfluidic platform for synthetic biology applications. United States: N. p., 2018.
Web.
Chang, Chieh, Bharadwa, Rajiv, Singh, Anup K., Chandrasekaran, Aarthi, & Hillson, Nathan J. Microfluidic platform for synthetic biology applications. United States.
Chang, Chieh, Bharadwa, Rajiv, Singh, Anup K., Chandrasekaran, Aarthi, and Hillson, Nathan J. Tue .
"Microfluidic platform for synthetic biology applications". United States. https://www.osti.gov/servlets/purl/1494577.
@article{osti_1494577,
title = {Microfluidic platform for synthetic biology applications},
author = {Chang, Chieh and Bharadwa, Rajiv and Singh, Anup K. and Chandrasekaran, Aarthi and Hillson, Nathan J.},
abstractNote = {This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to form a sequenced order of droplets carrying different components of the biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of the biological construct to permit the components to react with each other in one or more reactions contributing to the assembly of the biological construct.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {11}
}
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