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Title: Microfluidic platform for synthetic biology applications

Abstract

This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to form a sequenced order of droplets carrying different components of the biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of the biological construct to permit the components to react with each other in one or more reactions contributing to the assembly of the biological construct.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1494577
Patent Number(s):
10,131,903
Application Number:
13/437,727
Assignee:
The Regents of the University of California (Oakland, CA); Sandia Corporation (Livermore, CA)
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Apr 02
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chang, Chieh, Bharadwa, Rajiv, Singh, Anup K., Chandrasekaran, Aarthi, and Hillson, Nathan J. Microfluidic platform for synthetic biology applications. United States: N. p., 2018. Web.
Chang, Chieh, Bharadwa, Rajiv, Singh, Anup K., Chandrasekaran, Aarthi, & Hillson, Nathan J. Microfluidic platform for synthetic biology applications. United States.
Chang, Chieh, Bharadwa, Rajiv, Singh, Anup K., Chandrasekaran, Aarthi, and Hillson, Nathan J. Tue . "Microfluidic platform for synthetic biology applications". United States. https://www.osti.gov/servlets/purl/1494577.
@article{osti_1494577,
title = {Microfluidic platform for synthetic biology applications},
author = {Chang, Chieh and Bharadwa, Rajiv and Singh, Anup K. and Chandrasekaran, Aarthi and Hillson, Nathan J.},
abstractNote = {This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to form a sequenced order of droplets carrying different components of the biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of the biological construct to permit the components to react with each other in one or more reactions contributing to the assembly of the biological construct.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {11}
}

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Works referenced in this record:

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