Title: Microbial consortia for programmable output via photoautotroph-heterotroph interactions

Abstract

Self-sustained, safe, stable and scalable microbial consortia (S⁵MicroCon) are described. The microbial consortia are regulated by photoautotroph-heterotroph interactions and RNA aptamer-based gene circuits. A rapid, high-throughput method for engineering RNA aptamer-based gene circuits (e.g. riboswitches) is also described.

Inventors:

Beliaev, Alex S.; McClure, Ryan S.; Bernstein, Hans C.; Lindemann, Stephen R.; Jansson, G. Chris

Issue Date:

2018-11-13

Research Org.:

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1494121

Patent Number(s):

10125348

Application Number:

14/991,263

Assignee:

Battelle Memorial Institute (Richland, WA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/195 - from bacteria
C07K14/245 - Escherichia

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N1/00 - Microorganisms, e.g. protozoa
C12N1/20 - Bacteria {
C12N1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors
C12N15/1093 - {General methods of preparing gene libraries, not provided for in other subgroups}
C12N15/115 - Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith {
C12N15/635 - {Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline}
C12N15/70 - Vectors or expression systems specially adapted for E. coli
C12N15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
C12N2310/16 - Aptamers
C12N2320/13 - in a process of directed evolution, e.g. SELEX, acquiring a new function

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6874 - involving nucleic acid arrays, e.g. sequencing by hybridisation

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DOE Contract Number:  

AC05-76RL01830

Resource Type:

Patent

Resource Relation:

Patent File Date: 2016 Jan 08

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES