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Title: Microbial consortia for programmable output via photoautotroph-heterotroph interactions

Abstract

Self-sustained, safe, stable and scalable microbial consortia (S5MicroCon) are described. The microbial consortia are regulated by photoautotroph-heterotroph interactions and RNA aptamer-based gene circuits. A rapid, high-throughput method for engineering RNA aptamer-based gene circuits (e.g. riboswitches) is also described.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1494121
Patent Number(s):
10,125,348
Application Number:
14/991,263
Assignee:
Battelle Memorial Institute (Richland, WA)
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 Jan 08
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Beliaev, Alex S., McClure, Ryan S., Bernstein, Hans C., Lindemann, Stephen R., and Jansson, G. Chris. Microbial consortia for programmable output via photoautotroph-heterotroph interactions. United States: N. p., 2018. Web.
Beliaev, Alex S., McClure, Ryan S., Bernstein, Hans C., Lindemann, Stephen R., & Jansson, G. Chris. Microbial consortia for programmable output via photoautotroph-heterotroph interactions. United States.
Beliaev, Alex S., McClure, Ryan S., Bernstein, Hans C., Lindemann, Stephen R., and Jansson, G. Chris. Tue . "Microbial consortia for programmable output via photoautotroph-heterotroph interactions". United States. https://www.osti.gov/servlets/purl/1494121.
@article{osti_1494121,
title = {Microbial consortia for programmable output via photoautotroph-heterotroph interactions},
author = {Beliaev, Alex S. and McClure, Ryan S. and Bernstein, Hans C. and Lindemann, Stephen R. and Jansson, G. Chris},
abstractNote = {Self-sustained, safe, stable and scalable microbial consortia (S5MicroCon) are described. The microbial consortia are regulated by photoautotroph-heterotroph interactions and RNA aptamer-based gene circuits. A rapid, high-throughput method for engineering RNA aptamer-based gene circuits (e.g. riboswitches) is also described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {11}
}

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Works referenced in this record:

Inference of interactions in cyanobacterial–heterotrophic co-cultures via transcriptome sequencing
journal, April 2014

  • Beliaev, Alexander S.; Romine, Margie F.; Serres, Margrethe
  • The ISME Journal, Vol. 8, Issue 11
  • DOI: 10.1038/ismej.2014.69

Engineering microbial consortia: a new frontier in synthetic biology
journal, September 2008


Engineering microbial consortia for controllable outputs
journal, March 2016

  • Lindemann, Stephen R.; Bernstein, Hans C.; Song, Hyun-Seob
  • The ISME Journal, Vol. 10, Issue 9
  • DOI: 10.1038/ismej.2016.26

A High-Throughput Screen for Synthetic Riboswitches Reveals Mechanistic Insights into Their Function
journal, February 2007


Engineered bidirectional communication mediates a consensus in a microbial biofilm consortium
journal, October 2007

  • Brenner, K.; Karig, D. K.; Weiss, R.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 44
  • DOI: 10.1073/pnas.0704256104

Programmed population control by cell–cell communication and regulated killing
journal, April 2004

  • You, Lingchong; Cox, Robert Sidney; Weiss, Ron
  • Nature, Vol. 428, Issue 6985, p. 868-871
  • DOI: 10.1038/nature02491

Synthetic Escherichia coli consortia engineered for syntrophy demonstrate enhanced biomass productivity
journal, January 2012


Emergent Biosynthetic Capacity in Simple Microbial Communities
journal, July 2014


Riboswitches: Structures and Mechanisms
journal, October 2010

  • Garst, A. D.; Edwards, A. L.; Batey, R. T.
  • Cold Spring Harbor Perspectives in Biology, Vol. 3, Issue 6
  • DOI: 10.1101/cshperspect.a003533

Co-culture systems and technologies: taking synthetic biology to the next level
journal, July 2014

  • Goers, Lisa; Freemont, Paul; Polizzi, Karen M.
  • Journal of The Royal Society Interface, Vol. 11, Issue 96
  • DOI: 10.1098/rsif.2014.0065

Design of synthetic microbial communities for biotechnological production processes
journal, August 2014


Engineering a portable riboswitch-LacP hybrid device for two-way gene regulation
journal, July 2011

  • Jin, Y.; Huang, J.-D.
  • Nucleic Acids Research, Vol. 39, Issue 19, p. e131-e131
  • DOI: 10.1093/nar/gkr609

Abundance and functional diversity of riboswitches in microbial communities
journal, January 2007

  • Kazanov, Marat D.; Vitreschak, Alexey G.; Gelfand, Mikhail S.
  • BMC Genomics, Vol. 8, Issue 1
  • DOI: 10.1186/1471-2164-8-347

A flow cytometry-based screen for synthetic riboswitches
journal, November 2008

  • Lynch, Sean A.; Gallivan, Justin P.
  • Nucleic Acids Research, Vol. 37, Issue 1, p. 184-192
  • DOI: 10.1093/nar/gkn924

How to Make a Synthetic Multicellular Computer
journal, February 2014


An efficient platform for genetic selection and screening of gene switches in Escherichia coli
journal, February 2009

  • Muranaka, Norihito; Sharma, Vandana; Nomura, Yoko
  • Nucleic Acids Research, Vol. 37, Issue 5
  • DOI: 10.1093/nar/gkp039

Engineering microbial systems to explore ecological and evolutionary dynamics
journal, October 2012

  • Tanouchi, Yu; Smith, Robert P.; You, Lingchong
  • Current Opinion in Biotechnology, Vol. 23, Issue 5, p. 791-797
  • DOI: 10.1016/j.copbio.2012.01.006

Engineered riboswitches: Expanding researchers' toolbox with synthetic RNA regulators
journal, February 2012


Succinate production from xylose-glucose mixtures using a consortium of engineered Escherichia coli
journal, October 2014

  • Xia, Tian; Altman, Elliot; Eiteman, Mark A.
  • Engineering in Life Sciences, Vol. 15, Issue 1, p. 65-72
  • DOI: 10.1002/elsc.201400113

A synthetic Escherichia coli predator–prey ecosystem
journal, January 2008

  • Balagaddé, Frederick K.; Song, Hao; Ozaki, Jun
  • Molecular Systems Biology, Vol. 4, Issue 1
  • DOI: 10.1038/msb.2008.24